Stress biomarkers in rats submitted to swimming and treadmill running exercises

Contarteze, Ricardo Vinícius Ledesma; Manchado, Fúlvia de Barros; Gobatto, Cláudio Alexandre; Mello, María Alice Rostom de

doi:10.1016/j.cbpa.2007.03.005

Cited by 154 publications

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“…Chronic stress also affects bone turnover, leading to "lowturnover" osteoporosis (12,13,56,61). Involuntary forms of exercise such as swimming and forced treadmill running increase serum biomarkers of stress in rats (15), and even voluntary wheel running has been shown to increase corticosterone secretion (7,21). Conversely, exercise that causes increased corticosteroid levels may also reduce adrenal activity in response to external stressors (1,20).…”

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confidence: 99%

Short-term voluntary exercise in the rat causes bone modeling without initiating a physiological stress response

Rosa¹,

Firth²,

Blair³

et al. 2010

American Journal of Physiology-Regulatory, Integrative and Comp

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research has revealed a neuroendocrine connection between the skeleton and metabolism. Exercise alters both bone modeling and energy balance and may be useful in further developing our understanding of this complex interplay. However, research in this field requires an animal model of exercise that does not cause a physiological stress response in the exercised subjects. In this study, we develop a model of short-term voluntary exercise in the female rat that causes bone modeling without causing stress. Rats were randomly assigned to one of three age-matched groups: control, tower climbing, and squat exercise (rising to an erect bipedal stance). Exercise for 21 days resulted in bone modeling as assessed by peripheral quantitative computed tomography. Fecal corticosterone output was used to assess physiological stress at three time points during the study (preexercise, early exercise, and late in the exercise period). There were no differences in fecal corticosterone levels between groups or time points. This model of voluntary exercise in the rat will be useful for future studies of the influence of exercise on the relationship between skeletal and metabolic health and may be appropriate for investigation of the developmental origins of those effects. computed tomography; corticosterone; exercise; metabolism; stress RECENT ADVANCES IN OUR UNDERSTANDING of the dynamic relationship between body structure and metabolism have revealed a neuroendocrine connection between bone remodeling and energy balance, with both bone and fat acting as endocrine organs (14). The osteoblast-specific protein osteocalcin regulates glucose handling through effects on insulin secretion and sensitivity (33). This action is in turn mediated by the adipokine leptin, which inhibits insulin secretion through direct effects on pancreatic ␤-cells (18) and through inhibition of osteocalcin activity (25). The interplay between bone and metabolism has recently been further elucidated by the discovery that the Forkhead family transcription factor FoxO1, a key regulator of insulin handling in multiple tissues, also acts within the osteoblast to control osteocalcin expression and bioactivity (46).Exercise stimulates modeling of the musculoskeletal system (19, 55, 65), alters metabolism (40), and enhances brain function (17), and thus exercise provides an excellent means of exploring the neuroendocrine connection between bone, fat, and energy balance. The rat model of exercise has been used extensively to study the physiological effects of physical activity using such exercise modalities as swimming (24, 32), running (20,29,41,49,52), jumping (57), tower climbing (43), rising to an erect bipedal stance (11, 64), and weight lifting (62). The effects of exercise on bone have been widely characterized in the rat. Ovariectomized rats undergo changes in bone structure that are nearly identical to those seen in human osteoporosis (28,42), and these animals have proven useful for the study of exercise interventions in osteoporosis treatment (36).In examini...

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mentioning

confidence: 99%

Short-term voluntary exercise in the rat causes bone modeling without initiating a physiological stress response

Rosa¹,

Firth²,

Blair³

et al. 2010

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Loading animals in a treadmill enable to adjust the intensity of work, and this regime is considered most appropriate for small laboratory animals. [13] In addition, such loads correspond to the high level of intensity of physical work, and it is performed by activating the aerobic-anaerobic energy path, which in this case was the most appropriate for assessment of metabolic changes in the test animals. [1,7,14] On the 15 th day of the experiment, the rats from 2 to 4 groups were loaded with running at a speed of 42 m/min and slope angle 10°.…”

Section: Methodsmentioning

confidence: 99%

“…In the first phase of the experiment (half rats from 2 to 4 groups, 7 subjects from each group), we investigated the physical capacity against duration of the race until complete exhaustion, confirmed by no reaction to stimulation with electrical charges (40 V) at the starting line of a treadmill. [13] The remaining 7 animals from groups 2 to 4 (2 nd phase of the experiment) were loaded with treadmill running under the same conditions for 10 min 3-5 min after exercise all the rats from the 2 nd phase of experiment and the intact rats (Group 1) were taken samples of biological material for the study of energy metabolism indicators.…”

Section: Methodsmentioning

confidence: 99%

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Toziuk¹

2017

IJGP

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Introduction: We investigated changes in the energy state of the skeletal muscle cells in rats under physical load (15 days). Materials and Methods: The experiments were performed on nonlinear white rats. On the 15 th day of the experiment, the rats were loaded with running at a speed of 42 m/min and slope angle 10°. The test substance sodium 2-(tetrazol[1,5-c]quinazolin-5-ylthio)acetate (compound КВ-28) at a dose of 1.7 mg/kg and reference compound 2-ethylthiobenzimidazole hydrobromide (32.0 mg/kg) were administered to animals intraperitoneally 60 min before treadmill training. Concentration of adenine nucleotides (ATP, ADP, and AMP) was determined in skeletal muscles by thin layer chromatography test. Results and Discussion: It was first found that a course of intraperitoneal injection of sodium 2-(tetrazol[1,5-c]quinazolin-5-ylthio)acetate (compound KB-28) contributed to 88.5% (P < 0.05) growth of physical endurance. We proved, that unlike the trained control, administration of compound KB-28 prevented imbalance in the system of adenine nucleotides. It was indicated by a significantly important increase of the level of ATP by 54.8% while parallel reducing concentration of ADP and AMP by 16.8 and 43.4%, respectively. Conclusions: Restoration of balance in the adenine nucleotides system may be one of the components of sodium 2-(tetrazol[1,5-c]quinazolin-5-ylthio)acetate (compound KB-28) actoprotective effect.

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“…Andrii Saienko 1 The objective of the study was to assess the effect of a sodium salt 3-(2-oxo-3-phenyl-2H- [1,2,4]triazine [2,3-c]quinazolin-6-yl) of propanoic acid (MT-279 compound) on physical endurance of rats under conditions of chronic hypokinesia (15 days) in comparison with 2-ethylthiobenzimidazole hydrobromide (2-ETBI).…”

Section: Assessment Of Mt-279 Compound Effect On Physical Endurance Omentioning

confidence: 99%

“…In the previous studies of 1,2,4-triazine-quinazoline derivatives, we established the presence of their expressive ability to increase physical endurance of rats under normal and complicated conditions. We experimentally identified the most active compound -a sodium salt 3-(2-oxo-3-phenyl-2H- [1,2,4] triazine [2,3-c]quinazolin-6-yl) of propanoic acid (MT-279 compound) [13]. Structural formula of MT-279 compound is given on figure 2.…”

Section: Assessment Of Mt-279 Compound Effect On Physical Endurance Omentioning

confidence: 99%

Assessment of MT-279 compound effect on physical endurance of rats under conditions of chronic hypokinesia

Saienko

Волощук

Toziuk

et al. 2017

Current Issues in Pharmacy and Medical Sciences

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The objective of the study was to assess the effect of a sodium salt 3-(2-oxo-3-phenyl- 2H-[1,2,4]triazine[2,3-c]quinazolin-6-yl) of propanoic acid (MT-279 compound) on physical endurance of rats under conditions of chronic hypokinesia (15 days) in comparison with 2-ethylthiobenzimidazole hydrobromide (2-ETBI). The results of the conducted experiment showed that chronic hypokinesia (15 days) caused a progressive decrease in the physical endurance of animals. The signs of this phenomenon occurred already on the Day 7 of the experiment. It manifested by a significant decrease in the duration of rats swimming, treadmill runtime and holding on rotating rods (rotarod test). The course of MT-279 compound (2.5 mg/kg, ip) administered to animals in the specified experimental conditions contributed to restoration of physical performance of the study subjects. Upon introduction of the compound, the duration of rats` swimming on Day 7 of the experiment increased by 108.2%, and on Day 15 - by 64.2%. Administration of 2-ETBI caused the growth of this indicator by 70.5% and 24.2%, respectively. Along with this, we revealed an increase of rats` treadmill running time. On Day 7 and Day 15 of the experiment, we recorded 55.2% and 68.4% growth of the indicator, respectively. Daily administration of 2-ETBI contributed to 41.7% and 59.7% increase of running time, respectively. On Day 7 of MT-279 compound administration under the conditions of chronic hypokinesia, the duration of rats` rod retention time increased by 58.7% compared to 6.2% for 2-ETBI. On Day 15 of the experiment, the physical endurance of animals assessed under this test compared to the control group was 89.0% and 72.3%, respectively. On Day 7, MT-279 compound was significantly superior to the comparator drug in all tests performed for assessment of the ability to improve physical endurance under conditions of chronic hypokinesia. On Day 15 of study, the statistically significant superiority of MT-279 compound over 2-ETBI was identified in all forced swimming and rotarod tests.

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Stress biomarkers in rats submitted to swimming and treadmill running exercises

Cited by 154 publications

References 30 publications

Short-term voluntary exercise in the rat causes bone modeling without initiating a physiological stress response

Short-term voluntary exercise in the rat causes bone modeling without initiating a physiological stress response

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Assessment of MT-279 compound effect on physical endurance of rats under conditions of chronic hypokinesia

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