Nurses' pregnancy may lead to burnout. These factors of perceived organizational support, coping style, depression anxiety and stress and work and family conflict have been widely researched, although not among pregnant nurses. The findings will expand the literature in this area. To explore the correlation among burnout syndromes of pregnant nurses from 12 public tertiary hospitals in Hainan Province and their perceived organizational support, simple style, depression anxiety stress and work-family conflict, a convenient sampling method was used to select Pregnant nurses, and a questionnaire survey was conducted using the General Questionnaire, Maslach Burnout Inventory-Human Services Survey (MBI-HSS), Perceived Organizational Support Scale (POSS), Simple Coping Style Scale (SCSS), Depression Anxiety Stress and Work and Family Conflict Scale (WAFCS) for pregnant nurses. We found that the pregnant nurses had high burnout level. POSS, SCSS, DASS-21 and WFCS were the influencing factors of burnout. The overall burnout syndromes of pregnant nurses from 12 public tertiary hospitals in Hainan Province are relatively worse. When conducting intervention programs, factors of perceived organizational support, coping style, depression anxiety and stress and work and family conflict should be taken into account.