Streptozotocin-Diabetes Impairs Prolactin Binding to Leydig Cells in Prepubertal and Pubertal Rats

Murthy, Sudha; Br, Sankar; Valli, G; Govindarajulu, P.; Balasubramanian, K.

doi:10.1055/s-2007-978800

Cited by 15 publications

(19 citation statements)

References 18 publications

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“…Their finding showed that insulin applied to maternal diabetic rats restored prostate histology and secretion (Santos et al, 2014). Partial maintenance of serum androgens such as testosterone, estradiol, and prolactin in insulin treated rats might be responsible for the maintenance of the histoarchitecture of the ventral prostate as reported by Sudha et al (1999).…”

Section: Kamani M; Mhabadi J A; Atlasi M A; Seyedi F; Kamanmentioning

confidence: 88%

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

et al. 2017

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KAMANI, M.; MHABADI, J. A.; ATLASI, M. A.; SEYEDI, F.; KAMANI, E. & NIKZAD, H.Protective effect of alcoholic extract of garden cress seeds on the histopathological changes of the ventral prostate in streptozotocin diabetic rats. Int. J. Morphol., 35(3):1178Morphol., 35(3): -1184Morphol., 35(3): , 2017. SUMMARY:Diabetes mellitus is a common serious metabolic illness occurring worldwide that may lead to male infertility. Various plants have been used in the treatment of diabetes. In this study, the effect of garden cress (Lepidium sativum) seed extract on fasting blood sugar is assessed for its protective effect on histopathological changes in the ventral prostate gland of streptozotocine-induced diabetic rats. Fifty adult male Wistar rats were randomly selected into five groups. Group 1 was the control placebo group where rats received only 0.1 mL normal saline via gastric gavages. Rats in Group 2 received an intraperitoneal injection of STZ 60 mg/kg body weight and those with FBS >250 mg/dL were considered diabetic. In Group 3, diabetic rats received insulin (3 U/100 g body weight) while in Groups 4 and 5 diabetic rats received 0.1 ml of 200 and 400 mg/kg respectively of an ethanol extract of Lepidium sativum seeds by gavage daily. The prostate was removed and weighed before transfer to Bouin's solution for histological studies. Administration of the 200 and 400 mg/kg doses of Lepidium sativum seed extract increased epithelium height and decreased interstitial volume density and fibromuscular thickness of the prostate significantly. Also, the volume density of the epithelium, fibro muscular, lumen, and interstitial tissues were changed significantly. The results suggest that Lepidium sativum seed extract has beneficial effects as a protective agent against the detrimental effects of diabetes on the reproductive system of diabetic male rats.

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Section: Kamani M; Mhabadi J A; Atlasi M A; Seyedi F; Kamanmentioning

confidence: 88%

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

et al. 2017

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“…This decrease in PRL could result in the reduced or delayed milk production observed in some diabetic women (Hartmann and Cregan, 2001;Neubauer et al 1990Neubauer et al , 1993Neville et al, 1988) and streptozotocin-induced diabetic rats (Ikawa et al, 1992;Lau et al, 1993). In diabetic males, decreased prolactin secretion has also been associated with sexual dysfunction (Sudha et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Ghrelin has both endocrine and non-endocrine activities including regulation of food intake and energy homeostasis (Wren et al, 2001), induction of adiposity (Tschop et al, 2000), actions on insulin secretion and glucose metabolism (Egido et al, 2002;Dezaki et al, 2006;Reimer et al, 2003) and stimulation of pituitary hormones including GH, prolactin and ACTH (Kojima et al, 1999;Arvat et al, 2001;Cheng et al, 1993;Broglio et al, 2003;Tassone et al, 2003). In addition, anti-inflammatory and anti-apoptotic actions of ghrelin have been reported in different organs and tissues Dixit et al, 2004;Granado et al, 2005;Granata et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Ghrelin treatment protects lactotrophs from apoptosis in the pituitary of diabetic rats

Granado¹,

Chowen²,

García‐Cáceres³

et al. 2009

Molecular and Cellular Endocrinology

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Poorly controlled diabetes is associated with hormonal imbalances, including decreased prolactin production partially due to increased lactotroph apoptosis. In addition to its metabolic actions, ghrelin inhibits apoptosis in several cell types. Thus, we analyzed ghrelin's effects on diabetes-induced pituitary cell death and hormonal changes. Six weeks after onset of diabetes in male Wistar rats (streptozotocin 70 mg/kg), mini-pumps infusing saline or 24 nmol ghrelin/day were implanted (jugular). Rats were killed two weeks later. Ghrelin did not modify body weight or serum glucose, leptin or adiponectin, but increased total ghrelin (p<0.05), IGF-I (p<0.01) and prolactin (p<0.01) levels. Ghrelin decreased cell death, iNOS and active caspase-8 (p<0.05) and increased prolactin (p<0.05), Bcl-2 (p<0.01) and Hsp70 (p<0.05) content in the pituitary. In conclusion, ghrelin prevents diabetes-induced death of lactotrophs, decreasing caspase-8 activation and iNOS content and increasing anti-apoptotic pathways such as pituitary Bcl-2 and Hsp70 and serum IGF-I concentrations.

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“…Circulating prolactin levels have been shown to be reduced in poorly controlled diabetes mellitus (Ikawa et al 1992, Montelongo et al 1992, Ostrom & Ferris 1993, Valimaki et al 1991 and diabetes can result in reduced lactating capabilities in both women and experimental animals (Ikawa et al 1992, Lau et al 1993, Ostrom & Ferris 1993, Neville & Morton 2001. In addition, decreased prolactin levels in diabetic animals have been associated with steroidogenic defects (Sudha et al 1999) and a decreased stress response (Ratner et al 1991), including the response to hypoglycemia (Kinsley et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Increased apoptosis of lactotrophs in streptozotocin-induced diabetic rats is followed by increased proliferation

Arroba

Frago

Argente

et al. 2006

Journal of Endocrinology

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Poorly controlled diabetes mellitus can result in decreased prolactin production and thus problems with lactation, reproduction, and other physiological processes. This may be due to a loss of lactotrophs, as we have previously shown that long-term (8 weeks) poorly controlled streptozotocininduced diabetes results in increased death of lactotrophs and that this most likely occurs through the activation of caspase-8 and the extrinsic cell death cascade. However, cell proliferation is also increased in the anterior pituitary at this time, although the cell type undergoing this proliferation and whether it is a response to the increased cell death remains unknown. In order to determine the time-course of increased cell death and proliferation in the anterior pituitary and if this is related to changes in tumor necrosis factor (TNF)-a, a cytokine involved in the activation of the extrinsic cell death pathway, rats were killed at 1, 4, 6, and 8 weeks after the induction of diabetes. Cell death was significantly increased after 4 weeks, as was caspase-8 activation, although circulating levels of TNF-a were increased as early as 1 week. Pituitary levels of TNF-a did not change significantly until 8 weeks after diabetes onset. Similarly, Western-blot analysis of proliferating cell nuclear antigen showed that anterior pituitary cell proliferation increased significantly 8 weeks after diabetes onset, with the majority of proliferating cells, as detected by BrdU incorporation, corresponding to lactotrophs. These results suggest that the increased death of lactotrophs in poorly controlled diabetic rats is followed by increased proliferation of this cell type, even when no treatment is given.

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Streptozotocin-Diabetes Impairs Prolactin Binding to Leydig Cells in Prepubertal and Pubertal Rats

Cited by 15 publications

References 18 publications

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

Ghrelin treatment protects lactotrophs from apoptosis in the pituitary of diabetic rats

Increased apoptosis of lactotrophs in streptozotocin-induced diabetic rats is followed by increased proliferation

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