Streptonigrin induces delayed chromosomal instability involving interstitial telomeric sequences in Chinese hamster ovary cells

Interstitial telomeric sequences are not preferentially involved in the chromosome damage induced by the methylating compound streptozotocin in chinese hamster cells

“…In good agreement with previous reports [Bolzán et al, 2001;Sánchez et al, 2009Sánchez et al, , 2010Mencucci et al, 2012;Vidal Bravo et al, 2012], cytogenetic analysis of untreated cultures of CHO cells after telomere PNA-FISH showed telomeric repeats, predominantly localized in the centromeric regions of the chromosomes (Fig. 1A).…”

“…Future studies on this area of research should aim to investigate the long-term effects of STZ on ITSs in order to determine whether this compound is able to induce delayed chromosomal instability involving ITSs, as previously observed for other antitumor antibiotics in CHO cells [Mencucci et al, 2012;Vidal Bravo et al, 2012], and telomere dysfunction (i.e., telomere FISH signal loss or duplication), as we recently observed in rat cells exposed to bleomycin [Paviolo et al, 2012]. Future studies on this area of research should aim to investigate the long-term effects of STZ on ITSs in order to determine whether this compound is able to induce delayed chromosomal instability involving ITSs, as previously observed for other antitumor antibiotics in CHO cells [Mencucci et al, 2012;Vidal Bravo et al, 2012], and telomere dysfunction (i.e., telomere FISH signal loss or duplication), as we recently observed in rat cells exposed to bleomycin [Paviolo et al, 2012].…”

“…We found that radiomimetic compounds are capable of inducing translocation and amplification of ITSs [Bolzán et al, 2001;Sánchez et al, 2009Sánchez et al, , 2010 and also breakage at centromeric regions rich in ITSs; however, these chromosome regions are not the preferential target for the clastogenic action of these compounds [Sánchez et al, 2009[Sánchez et al, , 2010. More recently, we showed that bleomycin and streptonigrin induce delayed instability of ITSs in CHO cells in the form of additional telomere FISH signals [Mencucci et al, 2012;Vidal Bravo et al, 2012]. In this study, we analyzed the relationship between ITSs and chromosomal aberrations induced by streptozotocin (STZ) in CHO cells 18 h after treatment to obtain further insight into the short-term effects of chemical mutagens on ITSs.…”

“…Previously, we investigated the effects of two radiomimetic compounds, bleomycin and streptonigrin, on ITSs from Chinese hamster ovary (CHO) cell lines [Bolzán et al, 2001;Sánchez et al, 2009Sánchez et al, , 2010. More recently, we showed that bleomycin and streptonigrin induce delayed instability of ITSs in CHO cells in the form of additional telomere FISH signals [Mencucci et al, 2012;Vidal Bravo et al, 2012]. More recently, we showed that bleomycin and streptonigrin induce delayed instability of ITSs in CHO cells in the form of additional telomere FISH signals [Mencucci et al, 2012;Vidal Bravo et al, 2012].…”

Unveiling the principle of microRNA-mediated redundancy in cellular pathway regulation

“…for studying cytogenetics and DNA repair mechanisms but also as host cells for the production of therapeutic proteins. 15,16 In order to investigate which miRNAs significantly affect fundamental cellular functions such as proliferation, apoptosis, necrosis or protein expression/secretion, we have separately introduced an entire murine miRNA mimics library into a recombinant CHO cell line constitutively expressing SEAP as model protein. Three days following transfection, cells were subjected to a high-content analysis to determine miRNA-induced changes in crucial cellular processes.…”