Streptomycetes associated with marine sponges collected from Andaman Sea and Its antibacterial activity

Baskaran, R.; Thenmozhi, S.; Kumar, Kamlendra; Mohan, P. M.; Vijayakumar, R.

doi:10.21161/mjm.150806

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…Strain Streptomyces avermitilis E03 15 is linked to the remaining strains. These results agree with Rintala et al (2001) who identified Streptomycetes by 16 S rRNA (Baskaran et al, 2017).…”

Section: Phylogenetic Analysis and Similarity Of 16s Rrna Sequence Genesupporting

confidence: 91%

“…Five isolates were very strong fungus antagonists and ten others were soft antagonistic. These results agree with Baskaran et al (2017) Streptomyces extracts from ten isolates were found to be bioactive against two bacteria. Actinobacteria found in sponges may also protect the sponge from bacteria, fungi, viruses, and other microflora by secreting secondary metabolites on the sponge's surface and inside.…”

Section: Effect On Linear Growth Of the Pathogenic Fungisupporting

confidence: 90%

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16S rRNA of Streptomyces isolates, mutagenic by EMS and antagonistic tested on Fusarium, Pythium and Rhizoctonia of cucumber plants

EL-shaer

EL-Zaway

Mohamed

2019

Archives of Agriculture Sciences Journal

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Cucumber is one of Egypt's most popular vegetables, although it is sensitive to a number of plant diseases. The current study aimed to develop superior Streptomyces bacteria for improved antagonistic to fungi using mutagenic agents. Two Streptomyces isolates were isolated from Egyptian soil and identified by biochemical methods according to Bergey's Manual and partial 16S rRNA sequencing. These isolates were phenotypically identified as Streptomycetes genera. Mutation experiment by using chemical ethyl methanesulfonate (EMS) mutation methods. Through mutagenesis by (EMS) out of 10 obtained mutants. The mutants of Streptomyces isolates gave height antagonism of cucumber plant fungi Fusarium, Pythium and Rhizoctonia on the plates selected and applied in pots experiments growing cucumber. Streptomyces isolates and their mutants gave high inhibition percentage for the three (the studied) pathogenic fungi compared with the control. In soil infested with Pythium deberianum and Rhizoctonia solani treatments with mutants 6, 7 and Topsin-M70 fungicide increased the percentage of survival plants by up to 85% compared with the control (40%). While the mutants 5. With Fusarium oxysporium 75 %. survival plants. While mutant 4 with Rhizoctonia solani and 2 and 9 Fusarium oxysporium had the lowest effect on increasing cucumber survival (55%) and mutant 2,8 on Pythium deberianum gave 60% compared with other treatments and control. This finding suggests that Streptomyces could be useful in protecting plants such as cucumbers from fungi and reducing environmental contamination with herbicides.

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“…Strain Streptomyces avermitilis E03 15 is linked to the remaining strains. These results agree with Rintala et al (2001) who identified Streptomycetes by 16 S rRNA (Baskaran et al, 2017).…”

Section: Phylogenetic Analysis and Similarity Of 16s Rrna Sequence Genesupporting

confidence: 91%

Section: Effect On Linear Growth Of the Pathogenic Fungisupporting

confidence: 90%

16S rRNA of Streptomyces isolates, mutagenic by EMS and antagonistic tested on Fusarium, Pythium and Rhizoctonia of cucumber plants

EL-shaer

EL-Zaway

Mohamed

2019

Archives of Agriculture Sciences Journal

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“…Efforts to treat or control bacterial infections have been carried out using antibiotics [3]. However, antibiotics can cause resistance if used in the long term.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of Nephrolepis biserrata extract against Aeromonas hydrophila and Vibrio parahaemolyticus

Maulianawati

Suharni

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Fish farming has become a significant sector in producing animal protein. However, a disease caused by bacteria such as Aeromonas hydrophila and Vibrio parahaemolyticus has become obstacle factors in these farming activities. Among the various procedures for preventing bacterial attacks, the use of natural ingredients deserves to be considered. This study aimed to test the ability of gray nail extract (Nephrolepis biserrata) in inhibiting the growth of A. hydrophila and V. parahaemolyticus in vitro. N. biserrata were extracted using methanol (EPM) and chloroform (EPC). EPM and EPC were then challenged with A. hydrophila and V. parahaemolyticus bacteria to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The test results showed that an inhibition zone of EPM against A. hydrophila and V. parahaemolyticus were 8.2 and 8.3 mm, respectively. EPC has been unable to inhibit the activity of both bacteria, whereas EPM could. The MIC value for both bacteria was 50 mg/mL, and MIB concentration was more than 50 mg/mL. The results of this study indicate that the N. biserrata extract could inhibit bacteria.

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“…These include rare Actinomycetes (Tioman island) [6]; polysaccharides producing Theonella sp. (Bidong island) [7]; pigmented bacterium (Tinggi Island) [8]; several cultivable bacterium (Bidong Island) [9]; and Streptomyces (Andaman sea) [10]; haloalkanoic acid degrading Bacillus aryabhattai [11]; and moderately halophilic lipase producing bacterium (Pahang coast) [12]. The potential of sponge associated microbes in bioprospecting of bioactive compound have been described [13], [14].…”

Section: Introductionmentioning

confidence: 99%

Pigmented Pseudoalteromonas Sp. Isolated from Marine Sponge with Anti-Microbial Activities against Selected Human Pathogens

Hamid

Rosmadi

2020

EJBIO

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Marine sponges have been the potential source of bioactive compounds with potent antimicrobial properties. Sponge associated microbes significantly provide the route of biosynthesis of some of these compounds. In this work, a total of 100 bacterial colonies were screened from a marine sponge from Class Demospongiae, which has been collected from Merambong Island, the state of Johor, Malaysia. In disk diffusion assay, only 2 out of 100 isolates; namely C40 and C52, were able to demonstrate active inhibitions against selected human pathogens (Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, except for Staphylococcus aureus). Isolates C40 and C52 were characterized to be Gram negative short rods, non-spore formers and catalase positive. Unlike the majority of other isolates from sponge which were Gram positive rods, Isolate C40 and C52 are Gram negative rods which grew in yellow pigmented colonies. Genotypic characterization using 16S ribosomal RNA sequencing were carried out on each isolate (accession number for C40 and C52 is MT645493 and MT645494, respectively). The 16S ribosomal RNA sequences revealed that these strains belonged to genus Pseudoalteromonas sp. with 97-98% similarities. Inhibitions studies showed that this sponge associated microorganisms potentially produce anti-microbial compounds useful for biotechnologies.

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Streptomycetes associated with marine sponges collected from Andaman Sea and Its antibacterial activity

Cited by 3 publications

References 40 publications

16S rRNA of Streptomyces isolates, mutagenic by EMS and antagonistic tested on Fusarium, Pythium and Rhizoctonia of cucumber plants

16S rRNA of Streptomyces isolates, mutagenic by EMS and antagonistic tested on Fusarium, Pythium and Rhizoctonia of cucumber plants

Antibacterial activity of Nephrolepis biserrata extract against Aeromonas hydrophila and Vibrio parahaemolyticus

Pigmented Pseudoalteromonas Sp. Isolated from Marine Sponge with Anti-Microbial Activities against Selected Human Pathogens

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