Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil

Mo, Ping; Yu, Yizun; Zhao, Jiarong; Gao, Jian

doi:10.1007/s10482-016-0797-z

“…Isolates from our soils were restricted to the genera Streptomyces, Arthrobacter , and Rhodococcus , as previously described ( Álvarez-López et al, 2015 ). Streptomyces was also isolated from Mn-contaminated soils ( Mo et al, 2017 ) and from the birch rhizosphere collected on the coal mining dump ( Ostash et al, 2013 ). The rhizosphere from the Ni-hyperaccumulating plant Alyssum serpyllifolium hosts Ni-resistant bacteria that were predominantly belonging to the Arthrobacter and Streptomyces genera ( Becerra-Castro et al, 2011b ).…”

Section: Resultsmentioning

confidence: 99%

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

Zappelini

¹

,

Álvarez-López

²

,

Capelli

³

et al. 2018

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The successful restoration of well-engineered tailings storage facilities is needed to avoid mine tailings problems. This study characterized the bacterial communities from vegetated and non-vegetated soils from a red gypsum landfill resulting from the industrial extraction of titanium. A set of 275 bacteria was isolated from vegetated soil and non-vegetated soil areas and taxonomically characterized using BOX-PCR. The study also evaluated the ability of a subset of 88 isolated bacteria on their ability to produce plant growth promoting (PGP) traits [indoleacetic acid (IAA) production, phosphate solubilization, and siderophore production] and their tolerance to potentially toxic elements (PTEs). Twenty strains were chosen for further analysis to produce inoculum for birch-challenging experiments. Principal component analysis (PCA) showed that the set of pedological parameters (pH, granulometry, carbon, organic matter, and Mg content) alone explained approximately 40% of the differences between the two soils. The highest density of total culturable bacteria was found in the vegetated soil, and it was much higher than that in the non-vegetated soil. The Actinobacteria phyla dominated the culturable soil community (70% in vegetated soil and 95% in non-vegetated soil), while the phyla Firmicutes (including the genus Bacillus) and Bacteroides (including the genera Pedobacter and Olivibacter) were found only in the vegetated soil fraction. Additional genera (Rhizobium, Variovorax, and Ensifer) were found solely in the vegetated soil. The vegetated soil bacteria harbored the most beneficial PGP bacteria with 12% of the isolates showing three or more PGP traits. The strains with higher metal tolerances in our study were Phyllobacterium sp. WR140 (RO1.15), Phyllobacterium sp. WR140 (R01.34), and Streptomyces sp. (R04.15), all isolated from the vegetated soil. Among the isolates tested in challenging experiments, Phyllobacterium (R01.34) and Streptomyces sp. (R05.33) have the greatest potential to act as PGP rhizobacteria and therefore to be used in the biological restoration of tailings dumps.

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“…In Table 3 , we depict novel heavy metal-resistant species which were isolated from metal-contaminated areas. The literature review ( Table S1 : List of strains from heavy metal-contaminated areas) suggests that many Streptomyces species with unique characteristics were isolated from soils of metal-contaminated areas [ 32 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. For example, Schmidt et al isolated streptomycetes highly resistant to nickel from a polluted site at the former uranium mine [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Cimermanova

¹

,

Pristaš

²

,

Piknová

³

2021

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Technosols are artificial soils generated by diverse human activities and frequently contain toxic substances resulting from industrial processes. Due to lack of nutrients and extreme physico-chemical properties, they represent environments with limited bacterial colonization. Bacterial populations of technosols are dominated usually by Actinobacteria, including streptomycetes, known as a tremendous source of biotechnologically important molecules. In this study, the biodiversity of streptomycete-like isolates from several technosols, mainly mine soils and wastes (landfills and sludge) in Slovakia, was investigated. The combination of basic morphological and biochemical characterisations, including heavy metal resistance determination, and molecular approaches based on 16S rRNA gene analysis were used for the identification of the bacterial strains. From nine isolates of Actinobacteria collected from different habitats, one was found to represent a new species within the Crossiella genus. Eight other isolates were assigned to the genus Streptomyces, of which at least one could represent a new bacterial species. Some isolates showed high resistance to Pb, Zn, Cu or Ni. The most tolerated metal was Pb. The results obtained in this study indicate that technosols are a prospective source of new actinomycete species resistant to heavy metals what underlines their bioremediation potential.

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“…Following surface sterilization, the leaves were ground with 9.0 ml sterilized water, and the homogenate (100 µl) was spread on modified Gause’s synthetic agar medium [7] and incubated for 2 weeks at 28 °C. Strain T44 T was isolated from rhizosphere soil, as described by Mo et al [7]. The purified strains GY16 T and T44 T were prepared for short-term preservation on Gause’s synthetic agar medium [24] slopes at 4 °C and suspended in sterile 30 % (w/v) glycerol solution for long-term conservation at −80 °C.…”

Section: Full-textmentioning

confidence: 99%

“…Therefore, more attention is being focused on Streptomyces strains from unusual and unexplored environments, such as soil polluted by heavy metals [7][8][9][10][11][12], salt lakes [13], seawater [14][15][16], animals [17,18], plants [19,20] and rhizosphere soil [21,22]. During an investigation into the diversity of endophytic and rhizosphere soil actinobacteria from Broussonetia papyrifera, hundreds of strains were isolated by using different media.…”

mentioning

confidence: 99%

“…Strain GY16 T was isolated from surface-sterilized leaves using the five-step procedure described by Qin et al [23]. Following surface sterilization, the leaves were ground with 9.0 ml sterilized water, and the homogenate (100 µl) was spread on modified Gause's synthetic agar medium [7] and incubated for 2 weeks at 28 °C. Strain T44 T was isolated from rhizosphere soil, as described by Mo et al [7].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Streptomyces phaeolivaceus sp. nov. and Streptomyces broussonetiae sp. nov., isolated from the leaves and rhizosphere soil of Broussonetia papyrifera

Mo

¹

,

Liu

²

,

Zhao

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Two novel actinobacteria, designated strains GY16T and T44T, were isolated from the leaves and rhizosphere soil of Broussonetia papyrifera, respectively. A polyphasic approach was used for determining their taxonomic position. Results of 16S rRNA gene sequence analysis indicated that strain GY16T exhibited highest similarities to Streptomyces cinereoruber subsp. fructofermentans CGMCC 4.1593T (98.82 %), Streptomyces deccanensis KCTC 19241T (98.76 %), Streptomyces scabiei NRRL B-16523T (98.69 %), Streptomyces europaeiscabiei KACC 20186T (98.69 %) and Streptomyces rishiriensis NBRC 13407T (98.69 %), and strain T44T showed 99.2, 99.1, 99.1 and <98.7 % sequence similarities to Streptomyces filipinensis CGMCC 4.1452T, Streptomyces achromogenes subsp. achromogenes DSM 40028T, Streptomyces durhamensis DSM 40539T and other Streptomyces species, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GY16T formed an independent subclade, which indicated that strain GY16T should belong to a potential novel species; and strain T44T was closely related to S. filipinensis CGMCC 4.1452T, S. achromogenes subsp. achromogenes DSM 40028T, S. durhamensis DSM 40539T and S. yokosukanensis DSM 40224T. However, the multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA–DNA hybridization values between closely related relatives were far from the species-level thresholds. In addition, phenotypic and chemotaxonomic characteristics further confirmed that strains GY16T and T44T belonged to two distinct species. Based on these results, it is concluded that the isolated strains represent novel species within the genus Streptomyces , for which the names Streptomyces phaeolivaceus sp. nov. (type strain GY16T=CICC 24807T=KCTC 49326T) and Streptomyces broussonetiae sp. nov. (type strain T44T=CICC 24819T=JCM 33918T) are proposed.

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Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil

Cited by 26 publications

References 32 publications

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

Streptomyces Dominate the Soil Under Betula Trees That Have Naturally Colonized a Red Gypsum Landfill

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

Streptomyces phaeolivaceus sp. nov. and Streptomyces broussonetiae sp. nov., isolated from the leaves and rhizosphere soil of Broussonetia papyrifera

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