Streptomyces species isolated from potato scabs in France: numerical analysis of “Biotype-100” carbon source assimilation data

DNA relatedness among strains of Streptomyces pathogenic to potato in France: description of three new species, S. europaeiscabiei sp. nov. and S. stelliscabiei sp. nov. associated with common scab, and S. reticuliscabiei sp. nov. associated with netted scab.

“…In a previous study (Bouchek-Mechiche et al, 1998), we showed that all pathogenic strains isolated in France from lesions of common scab on potatoes and other root vegetables were identified as S. scabies based on the following primary characteristics used to describe this species : grey spores borne in spiral chains, utilization of the nine ISP sugars (Shirling & Gottlieb, 1966), melanine production and sensitivity to streptomycin. We also demonstrated that S. scabies strains are phenotypically heterogeneous, and can be divided into three groups (subphena 1a, 1b and 1c) and unclustered strains, which were differentiated by the following biochemical test : assimilation of 1-o-methyl-α-galactopyranoside (OMPG), trans-aconitate, 5-keto--gluconate, betain, (j)trehalose and gentisate.…”

“…On the basis of phenotypic features (Bouchek-Mechiche et al, 1998), strains of netted scab formed a homogeneous group readily differentiated by various characteristics (pigmentation, morphology and carbon sources utilization) from other described pathogenic species of Streptomyces. These strains were pathogenic only on some susceptible cultivars of potato (data not shown), on which they produced typical symptoms of netted scab on the surface of tubers and on the roots.…”

“…All strains were originally isolated from scab symptoms on potato or other root crops. These strains have been phenotypically described and cluster into six phena with some unclustered strains, as indicated in Table 1 ( Bouchek-Mechiche et al, 1998). All strains were routinely cultured on yeast extract-malt extract agar (Pridham et al, 1956(Pridham et al, -1957, and were stored at k20 mC in tryptone-yeast extract broth (Pridham & Gottlieb, 1948) containing 20 % (v\v) glycerol.…”

Streptomyces species associated with common scab lesions of potatoes in South Africa

“…The structure of the spore chain and sometimes the spores are examined under a microscope. Physiological testing involves growth at different pH levels, production of melanin in the presence of tyrosine, utilization of different sugars and resistance to various antibiotics (Loria et al 1995;Lindholm et al 1997;Bouchek-Mechiche et al 1998). Genetically the isolates are screened for the presence of the pathogenicity island (PAI) genes and identified using different PCR based techniques, such as 16S rDNA sequencing, DNA-DNA hybridization and the use of species-specific primers (Leiminger et al 2012;Pánková et al 2012;Dees et al 2013).…”

Streptomyces luridiscabiei sp. nov., Streptomyces puniciscabiei sp. nov. and Streptomyces niveiscabiei sp. nov., which cause potato common scab disease in Korea

“…In addition, obscure values depend to some extent on the choice of which DNA is labelled and commonly used methods (Grimont et al, 1980). Nevertheless, DNA-DNA hybridization is the preferred method for defining highly related species (Wayne et al, 1987;Stackebrandt & Goebel, 1994;Bouchek-Mechiche et al, 2000).…”