DNA relatedness among strains of Streptomyces pathogenic to potato in France: description of three new species, S. europaeiscabiei sp. nov. and S. stelliscabiei sp. nov. associated with common scab, and S. reticuliscabiei sp. nov. associated with netted scab.

Bouchek-Mechiche, Karima; Gardan, Louis; Normand, Philippe; Jouan, B.

doi:10.1099/00207713-50-1-91

Cited by 128 publications

(103 citation statements)

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“…The best-known species, S. scabies, seems to be cosmopolitan in distribution, though several recent reports indicate that it is infrequent in Europe (Bouchek-Mechiche et al 2000;Flores-González et al 2008;Kreuze et al 1999). The most frequent species in northern Europe is S. europaeiscabiei (Flores-González et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The most frequent species in northern Europe is S. europaeiscabiei (Flores-González et al 2008). S. stelliscabiei has been reported from France and the USA (Bouchek-Mechiche et al 2000;Wanner 2006). Phylogenetically more distant CS-causing species are known from multiple regions, including S. turgidiscabies in Japan and Finland (Kreuze et al 1999;Miyajima et al 1998), and S. acidiscabies from Maine, Korea and Japan (Lambert and Loria 1989a;Song et al 2004;Tóth et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

Wanner

2009

Am. J. Pot Res

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To determine distribution and prevalence of Streptomyces species associated with common scab in North America, more than 1,400 isolates were made from scabby potato tubers. Samples were obtained from locations across the continent, and from multiple sites in close geographic proximity. Most potentially pathogenic isolates belonged to four species previously associated with common scab, S. scabies, S. europaeiscabiei, S. stelliscabiei, and S. sp. IdahoX. Additionally, small numbers of potentially pathogenic isolates were identified as S. acidiscabies, S. turgidiscabies and six additional ribotypes. S. europaeiscabiei was most common in the west and S. scabies predominated in the middle and eastern Midwest. Beyond this geographic trend, species distribution was patchy. A single species often predominated in a field while neighboring fields had different species. Local and regional variation was seen when isolates were genotyped for repetitive sequence element patterns, reflecting the conserved or core genome, and genes characteristic of the Streptomyces pathogenicity island, reflecting flexible components of the Streptomyces genome. This is the largest survey to date of the distribution of common scab-causing species, and will be a resource for population biology studies and for tracing the evolutionary history and spread of common scabassociated Streptomyces.Resumen Para determinar la distribución y prevalencia de especies de Streptomyces asociadas con la sarna común en Norteamérica, se hicieron más de 1,400 aislamientos de tubérculos de papa con sarna. Las muestras fueron obtenidas de localidades de todo el continente y de múltiples lugares geográficamente próximos. Los aislamientos potencialmente más patogénicos fueron de cuatro especies previamente asociadas con sarna común, S. scabies, S. europaeiscabiei, S. stellicabiei y S. sp. IdahoX. Adicionalmente, un número pequeño de aislamientos potencialmente patogénicos fueron identificados como S. acidiscabies, S. turgidiscabies y seis ribotipos adicionales. S. europaeiscabiei fue el más común en el oeste y S. scabies predominó en el centro y este del Medio Oeste. Más allá de esta tendencia geográfica, la distribución de especies fue en parches. Una sola especie a menudo predominó en un campo mientras en los campos vecinos se encontraron diferentes especies. Se vieron variaciones locales y regionales cuando a los aislamientos se les determinó el genotipo para patrones de elementos con secuencias repetitivas que reflejan el genoma conservado o central, y genes característicos de la isla de patogenicidad de Streptomyces, reflejando componentes flexibles del genoma de Streptomyces. Este es la pesquisa más extensa a la fecha sobre la distribución de las especies que causan sarna común, y será un recurso para estudios de biología poblacional y para determinar la historia evolutiva y diseminación de Streptomyces asociado a la sarna común.Keywords Streptomyces scabies . Population genetics . Pathogenicity island Abbreviations CSCommon scab PAI Pathogenicity islan...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

Wanner

2009

Am. J. Pot Res

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“…DNA relatedness studies (9), fatty acid profiles (23,24), and inhibitory reactions (23) between paired strains all support a model for genetic diversity among strains currently classified in this species. A comprehensive study of biochemical characteristics, DNA-DNA relatedness, and 16S ribosomal DNA (rDNA) sequence analysis placed S. scabiei strains into three genomic species (1,2). The species names S. europaeiscabiei and S. stelliscabiei were assigned to two of the genomic species, separating the S. scabiei phenetic cluster into three genomic species.…”

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confidence: 99%

Horizontal Transfer of the Plant Virulence Gene, nec1 , and Flanking Sequences among Genetically Distinct Streptomyces Strains in the Diastatochromogenes Cluster

Bukhalid

Takeuchi²,

Labeda

et al. 2002

Appl Environ Microbiol

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Evidence for the horizontal transfer of a pathogenicity island (PAI) carrying the virulence gene nec1 and flanking sequences among Streptomyces strains in the Diastatochromogenes cluster is presented. Plant-pathogenic, thaxtomin-producing Streptomyces strains, previously classified as S. scabiei based on the conventionally used phenotypic characteristics, were found to be genetically distinct from the type strain of S. scabiei based on DNA relatedness and 16S rDNA sequence analysis. Pairwise DNA-DNA hybridizations between some of these strains and the S. scabiei type strain were as low as 36%, a value much below what is conventionally accepted for species identity (70%). The sequence of the nec1 gene, however, was identical in all the S. scabiei and S. scabiei-like strains tested, irrespective of their DNA relatedness to the type strain of S. scabiei, their geographic origin, or the isolation host. Furthermore, a 26-kb DNA fragment including and flanking nec1 was also conserved among these strains based on restriction and Southern analyses. These data indicate that the etiology of potato scab is more complex than previously recognized; this result has important implications for potato scab management strategies. Previous research has suggested that horizontal transfer of a PAI was the mechanism for evolution of pathogenicity in S. acidiscabies and S. turgidiscabies, species that lie outside of the Diastatochromogenes cluster. Data presented here support this model and indicate that PAI transfer also has occurred frequently in species closely related to S. scabiei.Hundreds of soil-dwelling, saprophytic streptomycetes have been described, while relatively few species are known pathogens of plants (see reference 20 for a review); hence, pathogenicity is considered a rare phenotype in the genus Streptomyces. Streptomycetes are filamentous, gram-positive prokaryotes that produce an array of agriculturally and clinically important secondary metabolites. Members of this genus have a complex morphology: spores germinate to produce substrate mycelium from which aerial hyphae arise, which, in turn, fragment into spore chains.Three well-characterized, genetically diverse species, S. scabiei, S. acidiscabies, and S. turgidiscabies, cause scab symptoms on potato and tap root crops (20). S. scabiei (18) was the first species to be described and occurs worldwide. S. acidiscabies (17) and S. turgidiscabies (22) were first reported as a cause of potato scab in 1977 and 1996, respectively. In contrast to S. scabiei, S. acidiscabies and S. turgidiscabies have very limited geographic distributions, the northeastern United States and the island of Hokkaido in Japan, respectively. The similarity in symptoms and host range of the three pathogenic species suggests a common mechanism of pathogenicity. Members of S. scabiei, S. acidiscabies, and S. turgidiscabies are known to produce a family of dipeptide phytotoxins, thaxtomins (3,14,19), which have recently been shown to be required for disease development (10). Although these three sp...

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“…Bintje in France (Bouchek-Mechiche et al 2000a), and (3) the type strain of S. scabies (ATCC49173). In S. turgidiscabies strain 32, all probes generated strong signals, e.g., for the genes txtA, txtB, txtC, txtD and txtR involved in thaxtomin biosynthesis and gene nec1 involved in virulence (Fig.…”

Section: Microarray Analysis Of Thaxtomin Biosynthetic Genes Of S Tumentioning

confidence: 99%

“…isolated in France showed that strains isolated from netted scab tissue were different from the pathogens of common scab or agents isolated from russet scab in Canada (Bouchek-Mechiche et al 1998). The Streptomyces strains causing netted scab in France were described as a new species, S. reticuliscabiei (type strain DSM41804; CFBP 4531) (Bouchek-Mechiche et al 2000a, b), but were later found to belong to S. turgidiscabies (Bouchek-Mechiche et al 2006). This strain is characterized by the production of light grey spores that make flexuous chains, and the lack of melanin production (BouchekMechiche et al 2000a).…”

Section: Introductionmentioning

confidence: 99%

Phytotoxin produced by the netted scab pathogen, Streptomyces turgidiscabies strain 65, isolated in Sweden

et al. 2018

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Streptomyces spp. are a highly diverse group of bacteria most of which are soil-inhabiting saprophytes. A few are plant pathogens that produce a family of phytotoxins called thaxtomins and cause significant economic losses, e.g., by reducing the marketability of potato tubers (Solanum tuberosum). In northern Europe, S. scabies, S. turgidiscabies and S. europaeiscabiei are the most common plant pathogenic species. In this study, a Streptomyces strain isolated from a netted scab lesion on a tuber of potato cv. Bintje in northern Sweden was identified as S. turgidiscabies but was found to differ in the genomic region carrying genes required for thaxtomin biosynthesis. Our results showed that the strain did not produce thaxtomin but rather phytotoxin fridamycin E, which is an anthraquinone novel to plant pathogenic Streptomyces spp. Fridamycin E was shown to reduce or inhibit sprouting of potato microtubers in vitro. While fridamycin E is known to have antibiotic activity against Gram-positive bacteria, the inhibitory activity of fridamycin E on plant growth is a novel finding.

show abstract

DNA relatedness among strains of Streptomyces pathogenic to potato in France: description of three new species, S. europaeiscabiei sp. nov. and S. stelliscabiei sp. nov. associated with common scab, and S. reticuliscabiei sp. nov. associated with netted scab.

Cited by 128 publications

References 38 publications

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

A Patchwork of Streptomyces Species Isolated from Potato Common Scab Lesions in North America

Horizontal Transfer of the Plant Virulence Gene, nec1 , and Flanking Sequences among Genetically Distinct Streptomyces Strains in the Diastatochromogenes Cluster

Phytotoxin produced by the netted scab pathogen, Streptomyces turgidiscabies strain 65, isolated in Sweden

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