Streptomyces jietaisiensis sp. nov., isolated from soil in northern China

Abstract

An actinomycete, strain FXJ46 T , was isolated from cypress forest soil in northern China and shown to have chemotaxonomic and morphological properties consistent with streptomycetes. It developed greyish aerial mycelium and pinkish-brown substrate mycelium on oatmeal agar. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nucleotide variable c-region of this molecule showed that it formed a distinct (but closely associated) line with Streptomyces griseoaurantiacus DSM 40430 T in Streptomyces trees. However, the DNA-DNA relatedness between the two strains was only 48?8 %. A number of phenotypic properties also readily distinguished the isolate from S. griseoaurantiacus and related Streptomyces species with validly published names. It is proposed, therefore, that this organism be classified as a novel species of the genus Streptomyces, for which the name Streptomyces jietaisiensis sp. nov. is proposed. The type strain is FXJ46

Micrococcus yunnanensis sp. nov., a novel actinobacterium isolated from surface-sterilized Polyspora axillaris roots

“…The topology of the phylogenetic tree was evaluated by using the bootstrap resampling method of Felsenstein (1985) with 1000 replicates. DNA-DNA relatedness was studied using the fluorometric micro-well method (Ezaki et al, 1989;Christensen et al, 2000;He et al, 2005). Fluorescence intensities were measured using a fluorescence microplate reader (SpectraMax Gemini XPS; Molecular Devices).…”

“…Hybridizations were performed with four replications for each sample. The DNA-DNA hybridization rate was calculated according to the method of He et al (2005). The DNA-DNA hybridization values for strain YIM 65004 T with respect to M. luteus DSM 20030 T , M. endophyticus YIM 56238 T , M. antarcticus T2 T , M. lylae DSM 20315 T and M. flavus LW4 T were 65.4 % (SD, 4.6 %), 58.9 % (SD, 1.0 %), 32.0 % (SD, 3.2 %), 43.3 % (SD, 3.6 %) and 27.1 % (SD, 2.7 %), respectively.…”

Nocardia endophytica sp. nov., an endophytic actinomycete isolated from the oil-seed plant Jatropha curcas L.

“…The stability of the trees was assessed by performing bootstrap analyses (Felsenstein, 1985) of the neighbour-joining data based on 1000 resamplings. The level of DNA-DNA relatedness between strain KLBMP 1256 T and N. callitridis CAP 290 T was determined according to the fluorometric micro-well method (Ezaki et al, 1989;He et al, 2005).…”

Jiangella alba sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis

“…A bootstrap analysis was performed to assess the confidence limits of the branching (Felsenstein, 1985). DNA-DNA hybridization was performed using the fluorometric microwell method (Ezaki et al, 1989) with the modifications described by He et al (2005). The G+C content of the genomic DNA was determined using the HPLC method as described by Mesbah et al (1989).…”