Nocardia endophytica sp. nov., an endophytic actinomycete isolated from the oil-seed plant Jatropha curcas L.

Abstract

A novel actinomycete, designated strain KLBMP 1256T, was isolated from a surface-sterilized stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China, and was characterized to determine its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus Nocardia in the family Nocardiaceae, being most closely related to Nocardia callitridis CAP 290T (98.4 % similarity) and Nocardia nova JCM 6044T (97.5 %). Levels of 16S rRNA gene sequence similarity between strain KLBMP 1256T and the type strains of other recognized species of the genus Nocardia were less than 97 %. Chemotaxonomic data supported the affiliation of the new isolate to the genus Nocardia. However, the novel strain could be distinguished from its closest phylogenetic neighbour, N. callitridis CAP 290T, by a range of phenotypic properties. The combination of low DNA–DNA relatedness values and phenotypic differences from N. callitridis CAP 290T indicated that strain KLBMP 1256T represents a novel species of the genus Nocardia, for which the name Nocardia endophytica sp. nov. is proposed. The type strain is KLBMP 1256T ( = KCTC 19777T = CCTCC AA 2010004T).

Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species

“…Besides nitrogen-fixation, PGP bacteria may modulate plant shoot and root development by secretion of plant hormones or enzymes; such as auxin and 1-aminocyclopropane-1-carboxylate deaminase that reduces ethylene level in plant tissues; enhancement of resistance to pathogens through releasing volatile compounds; solubilisation of soil phosphorus and trace elements [19,21,43]. To date, a small number of endogenous bacteria species have been isolated from J. curcas , including Pseudonocardia sichuanensis [44], Nocardia endophytica [45], Pleomorphomonas diazotrophica [46], Aureimonas jatrophae and Aureimonas phyllosphaerae [47] , Jatrophihabitans endophyticus [48] and Enterobacter cancerogenus [49]. However, none has been studied on the mechanistic aspects of plant growth promotion in this promising biofuel plant.…”

The Bacterial Microbiome of Meloidogyne-Based Disease Complex in Coffee and Tomato

“…At the genera level, only Nocardia was shared by the corkyroot samples. Species of this genus are found worldwide in soils rich in organic matter, some of which are plant pathogenic (Xing et al, 2011;Bai et al, 2016;Conville et al, 2017). Nocardia sp.…”

Permanent Improved High-Quality Draft Genome Sequence of Nocardia casuarinae Strain BMG51109, an Endophyte of Actinorhizal Root Nodules of Casuarina glauca

“…However, several Nocardia spp. have been consistently reported as plant endophytes and isolated from disinfected roots ( N. callitridis [ 2 ]), stems ( N. artemisiae [ 3 ] and N. endophytica [ 4 ]), and actinorhizal root nodules ( N. autotrophica [ 5 ], N. casuarinae [ 6 ], and Nocardia sp. [ 7 – 9 ]).…”