Streptomyces genomes: circular genetic maps from the linear chromosomes

Wang, Shih-Jie; Chang, Hern-Jia; Lin, Yi-Shing; Huang, Chih‐Hung; Chen, Carton W.

doi:10.1099/00221287-145-9-2209

Cited by 29 publications

(33 citation statements)

References 31 publications

(36 reference statements)

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“…The hypothesis that map circularity deduced from haploid recombinant frequencies might be due to recovering only evennumbered crossover progeny was strikingly confirmed by a recent physical analysis of crosses between S. coelicolor and S. lividans, which have recognizably different chromosomal end sequences: Nearly all recombinants inherited both ends from the same parent (114 (124). Different regions of the S. coelicolor chromosome were tagged with fluorescently labeled probes, whereupon the two ends were nearly always seen adjacent in the vegetative mycelium.…”

Section: Merozygotementioning

confidence: 76%

“…Transient recombination of the plasmid with the chromosome would lead to chromosome transfer, probably as a loop, but whether transfer is normally complete or partial is unresolved. Wang et al (114) found both the unselected genetic markers and the telomeres of the recipient to be preferentially inherited in such crosses, but this does not prove incomplete transfer since backcrossing of recombinant genomes with recipient chromosomes during intramycelial spreading would lead to the same result.…”

Section: Figurementioning

confidence: 88%

“…For the two examples studied (114), recombinants in interspecific (S. coelicolor × S. lividans) crosses, unexpectedly, tended to inherit telomeres and nonselected markers from the donor. This is compatible with complete chromosome transfer, but the authors needed a special explanation for a failure to inherit recipient DNA, attributing it to active destruction of resident genomes, perhaps through invasion by transposons from the incoming genome released from repression on entering a naïve cytoplasm.…”

Section: Figurementioning

confidence: 99%

“…Unsurprisingly, these genomes were highly unstable, perhaps because they contained two competing oriC regions. Crosses between S. coelicolor and S. lividans generated rare recombinants stably inheriting one TIR from each parent and so differing except for a short terminal sequence (114), while perhaps S. avermitilis, with TIRs of only 168 bp (59), is such a naturally occurring hybrid (24). Likewise, S. lividans plasmid SLP2 is presumed to have originated by recombination between a progenitor plasmid and the S. lividans chromosome (58).…”

Section: Genome Instabilitymentioning

confidence: 99%

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Soil To Genomics: The Streptomyces Chromosome

2006

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The 8-9-Mb Streptomyces chromosome is linear, with a "core" containing essential genes and "arms" carrying conditionally adaptive genes that can sustain large deletions in the laboratory. Bidirectional chromosome replication from a central oriC is completed by "endpatching," primed from terminal proteins covalently bound to the free 5 -ends. Plasmid-mediated conjugation involves movement of double-stranded DNA by proteins resembling other bacterial motor proteins, probably via hyphal tip fusion, mediated by these transfer proteins. Circular plasmids probably transfer chromosomes by transient integration, but linear plasmids may lead the donor chromosome end-first into the recipient by noncovalent association of ends. Transfer of complete chromosomes may be the rule. The recipient mycelium is colonized by intramycelial spreading of plasmid copies, under the control of plasmid-borne "spread" genes. Chromosome partition into prespore compartments of the aerial mycelium is controlled in part by actin-and tubulin-like proteins, resembling MreB and FtsZ of other bacteria.

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Section: Merozygotementioning

confidence: 76%

Section: Figurementioning

confidence: 88%

Section: Figurementioning

confidence: 99%

Section: Genome Instabilitymentioning

confidence: 99%

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Soil To Genomics: The Streptomyces Chromosome

2006

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“…It is not clear whether the recombination occurred at all the ORF1 sequences together, or at a single site first and then spread to other ends by gene conversion. In any case, the high frequency of concurrent recombination events suggests that all these telomeres are in relatively close vicinity to one another in the cells, a notion that was proposed by Wang et al (1999) based on conjugation analysis.…”

Section: Orf1 Is Not Essential For Replication Of Linear Repliconsmentioning

confidence: 94%

The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid The GenBank accession number for the sequence determined in this work is AF194023.

Bey¹,

Tsou²,

Huang³

et al. 2000

Microbiology

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The chromosome of Streptomyces lividans shares 154 kb homology with one end of the linear plasmid SLP2, consisting of a 101 kb terminal sequence followed by the 53 kb transposable element Tn4811. The 101 kb terminal sequence was determined. The mean GMC content of this sequence is 679 mol % with a striking G vs C bias in the last kb. The terminal 232 nt contained 10 palindromic sequences with potential to form complex secondary structures. One typical Streptomyces coding sequence (designated ORF1) of 2643 bp was predicted in the determined sequence. The amino acid sequence of the ORF1 product contained a DEAH helicase motif, and exhibited similarity to type I restriction enzyme HsdR subunits in the database, suggesting a possible role in replication of the telomeres. However, all the ORF1 sequences on the chromosome and SLP2 could be simultaneously knocked out by targeted recombination without affecting the viability of the cells and the linearity of the chromosome and SLP2. This ruled out ORF1 as an essential component in the maintenance of the linear chromosome and plasmids.

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PlasmidDNAReplication

Tolmasky

Actis

Crosa

2010

Encyclopedia of Industrial Biotechnology

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Plasmids are extrachromosomal replicons found in gram‐negative and gram‐positive bacteria as well as in some lower eukaryotic organisms. They are present in bacterial cells replicating at a specific number of copies per cell. Their size varies from a few to several hundred kilobase pairs and bacterial cells can harbor more than one plasmid species. The medical importance of plasmids that code for antibiotic resistance and those that contribute directly to microbial pathogenicity is well‐documented, is the role played by plasmids in bacteria of importance in agriculture and industry. These extrachromosomal elements are of equal importance, however, for the study of the structure and function of DNA. Plasmids utilize a wide variety of strategies to initiate and regulate their replication. In this chapter we examine the replication of plasmids ColE1, whose replication system is part of most plasmids vectors; R6K, an iteron‐containing plasmid that possesses three replica origins; plasmids belonging to the RepABC family found in a‐proteobacteria; and of pT181, a group of plasmids found in gram‐positive bacteria. In addition,we also discuss linear plasmids.

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Streptomyces genomes: circular genetic maps from the linear chromosomes

Cited by 29 publications

References 31 publications

Soil To Genomics: The Streptomyces Chromosome

Soil To Genomics: The Streptomyces Chromosome

The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid The GenBank accession number for the sequence determined in this work is AF194023.

PlasmidDNAReplication

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