The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid The GenBank accession number for the sequence determined in this work is AF194023.

Bey, Shian-Jy; Tsou, Meng-Fu Bryan; Huang, Chih‐Hung; Yang, Chien-Chin; Chen, Carton W.

doi:10.1099/00221287-146-4-911

Cited by 24 publications

(23 citation statements)

References 54 publications

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“…3). The seven typical palindromes predicted to form the secondary structures found in other known Streptomyces telomeres are present in both strains (7,17) (Fig. 3).…”

Section: Resultsmentioning

confidence: 93%

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Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens

et al. 2006

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The sequences of the terminal inverted repeats (TIRs) ending the linear chromosomal DNA of two Streptomyces ambofaciens strains, ATCC23877 and DSM40697 (198 kb and 213 kb, respectively), were determined from two sets of recombinant cosmids. Among the 215 coding DNA sequences (CDSs) predicted in the TIRs of strain DSM40697, 65 are absent in the TIRs of strain ATCC23877. Reciprocally, 45 of the 194 predicted CDSs are specific to the ATCC23877 strain. The strain-specific CDSs are located mainly at the terminal end of the TIRs. Indeed, although TIRs appear almost identical over 150 kb (99% nucleotide identity), large regions of DNA of 60 kb (DSM40697) and 48 kb (ATCC23877), mostly spanning the ends of the chromosome, are strain specific. These regions are rich in plasmid-associated genes, including genes encoding putative conjugal transfer functions. The strain-specific regions also share a G؉C content (68%) lower than that of the rest of the genome (from 71% to 73%), a percentage that is more typical of Streptomyces plasmids and mobile elements. These data suggest that exchanges of replicon extremities have occurred, thereby contributing to the terminal variability observed at the intraspecific level. In addition, the terminal regions include many mobile genetic elementrelated genes, pseudogenes, and genes related to adaptation. The results give insight into the mechanisms of evolution of the TIRs: integration of new information and/or loss of DNA fragments and subsequent homogenization of the two chromosomal extremities.

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“…3). The seven typical palindromes predicted to form the secondary structures found in other known Streptomyces telomeres are present in both strains (7,17) (Fig. 3).…”

Section: Resultsmentioning

confidence: 93%

“…Chen in his "end-first" model (8). This model predicts that this probable helicase would be involved in conjugal transfer by acting on the DNA terminus which would correspond to an origin of transfer (7,8).…”

Section: Discussionmentioning

confidence: 99%

Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens

et al. 2006

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“…It is also possible that pREA100 integrated into the genome. This has been shown to be the case for the large plasmids of Streptomyces, which exhibit remarkable Xuidity and recombination potential (Bey et al, 2000). It would be very interesting to determine whether this plasticity is the case for rhodococci as well.…”

Section: Discussionmentioning

confidence: 93%

TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12

Yang

Lessard

Sengupta

et al. 2007

Plasmid

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Pulsed-Weld gel electrophoresis (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus erythropolis AN12. These megaplasmids, pREA400, pREA250, and pREA100, are approximately 400, 250, and 100 kb, respectively, based on their migration in pulsed-Weld gels. Genetic screening of an AN12 transposon insertion library showed that two megaplasmids, pREA400, and pREA250, are conjugative. Mobilization frequencies of these AN12 megaplasmids to recipient R. erythropolis SQ1 were determined to be approximately 7 £ 10 ¡4 and 5 £ 10 ¡4 events per recipient cell, respectively. It is known for other bacterial systems that a relaxase encoded by the traA gene is required to initiate DNA transfer during plasmid conjugation. Sequences adjacent to the transposon insertion in megaplasmid pREA400 revealed a putative traA-like open reading frame. A targeted gene disruption method was developed to generate a traA mutation in AN12, which allowed us to address the role of the traA gene product for Rhodococcus megaplasmid conjugation. We found that the AN12 traA mutant is no longer capable of transferring the pREA400 megaplasmid to SQ1. Furthermore, we conWrmed that the conjugation defect was speciWcally due to the disruption of the traA gene, as pREA400 megaplasmid conjugation defect is restored with a complementing copy of the traA gene.

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“…The linear plasmids replicate from a centrally located origin and carry proteins bound to the repetitive ends (10,222). These linear plasmids seem to recombine frequently with the linear chromosome (18), resulting in the exchange of a plasmid end and a chromosomal end. Since the chromosomal ends of Streptomyces do not contain essential genes, the loss of a chromosomal end usually does not interfere with the viability of the Streptomyces strain.…”

Section: Different Types Of Conjugative Streptomyces Plasmidsmentioning

confidence: 99%

Conjugative Plasmid Transfer in Gram-Positive Bacteria

Grohmann

Muth

Espinosa

2003

Microbiol Mol Biol Rev

503

447

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Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene spread, and it is therefore considered one of the major reasons for the increase in the number of bacteria exhibiting multiple-antibiotic resistance. Thus, conjugation and spread of antibiotic resistance represents a severe problem in antibiotic treatment, especially of immunosuppressed patients and in intensive care units. While conjugation in gram-negative bacteria has been studied in great detail over the last decades, the transfer mechanisms of antibiotic resistance plasmids in gram-positive bacteria remained obscure. In the last few years, the entire nucleotide sequences of several large conjugative plasmids from gram-positive bacteria have been determined. Sequence analyses and data bank comparisons of their putative transfer (tra) regions have revealed significant similarities to tra regions of plasmids from gram-negative bacteria with regard to the respective DNA relaxases and their targets, the origins of transfer (oriT), and putative nucleoside triphosphatases NTP-ases with homologies to type IV secretion systems. In contrast, a single gene encoding a septal DNA translocator protein is involved in plasmid transfer between micelle-forming streptomycetes. Based on these clues, we propose the existence of two fundamentally different plasmid-mediated conjugative mechanisms in gram-positive microorganisms, namely, the mechanism taking place in unicellular gram-positive bacteria, which is functionally similar to that in gram-negative bacteria, and a second type that occurs in multicellular gram-positive bacteria, which seems to be characterized by double-stranded DNA transfer

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The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid The GenBank accession number for the sequence determined in this work is AF194023.

Cited by 24 publications

References 54 publications

Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens

Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens

TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12

Conjugative Plasmid Transfer in Gram-Positive Bacteria

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