Streptomyces Antibiotics. IV. Hydrolytic Cleavage of Streptomycin to Streptidine

Abstract

Hydrolytic Cleavage of Streptomycin to Streptidine 29 2. The proportions of ester anion and amide ture of esters on these courses of reaction has been formed from certain esters and sodium amide discussed. have been determined. The influence of struc-Durham, N. C.

Inhibition of Root Growth by Streptomycin and Reversal of the Inhibition by Manganese

“…Another experiment was run using freshly hydrolyzed streptomycin in order to test streptidine and streptobiosamine together on root growth. Streptomycin was hydrolyzed at room temperature in methyl alcohol solution containing hydrochloric acid by the method of Peck et al (1946). The completion of the hydrolysis was determined when an assay of an aliquot of the solvent, using the streptomycin-dependent strain of Escherichia coli, Treatment Avg.…”

Rescue of the streptomycin antibiotic activity by using streptidine as a “decoy acceptor” for the aminoglycoside-inactivating enzyme adenyl transferase

“…The streptidine was subjected to enzymatic adenylation with ATP and recombinant ANT (6). Formation of the adenylated product (AMP-streptidine) was confirmed by 1 H-NMR, 13 C-NMR and (ES+) m/z. 14 The most relevant kinetic parameters for the recombinant ANT(6) are summarized in Table 1.…”

Therapeutic Agents Acting on RNA Targets

“…Nevertheless, streptomycin binds to 30S in the ram state and consequently stabilizes that state, which results in error-prone translation and thus the accumulation of miscoded proteins and the reduction of proteins with the correct sequence. [59]. Figure 2 highlights permissible and nonpermissible alterations to streptomycin and analogs.…”

“…Ring III (glucosamine) makes two direct contacts with 30S, neither of which are essential to activity [59,75]. Indeed, all of ring III can be dispensed with and cause only modest reductions in activity.…”