Streptococcus uberis ST439 and ST475 induce differential inflammatory responses in a mouse intramammary infection model

Mitra, Susweta Das; Shome, Bibek Ranjan; Mani, Bhuvana; Velu, Dhanikachalam; Banerjee, Apala; Bankar, Kiran; Ghosh, Sankar Kumar; Santra, Sandip; Suresh, Kuralayanapalya Puttahonnappa; Rahman, Hazir

doi:10.1016/j.gene.2016.03.054

Cited by 5 publications

(4 citation statements)

References 32 publications

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“…5,20 Previous studies have shown that the heterogeneity of S. uberis populations results in varied immune responses, and different virulence factors may promote these process. 8,14 In this study, we used three strains of S. uberis to explore their specific pathogenicity. The SU41 and supan03 strains belong to ST1276 and ST1281, respectively, which are reported here for the first time, whereas the HFJ5 strain belongs to ST 986.…”

Section: Discussionmentioning

confidence: 99%

“…Among these complexes, CC5 is associated with acute mastitis, CC143 is linked to recessive mastitis, and CC186 is linked to latent infections. 9 Different sequence type (ST) strains induce different inflammatory responses, 14 hence, it is of great significance to explore the pathogenicity of additional ST strains. S. uberis possesses different virulence factors, including plasminogen activator, 15 S. uberis adhesion molecule, 12 hyaluronic acid capsule, 16 and lactoferrin-binding protein (LBP).…”

Section: Introductionmentioning

confidence: 99%

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Immune response variations and intestinal flora changes in mastitis induced by three Streptococcus uberis strains

Zhou

Qiu

et al. 2022

Microbiology and Immunology

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Streptococcus uberis is a common cause of mastitis. The pathogenicity among different strains of S. uberis and the resultant host immune responses remain to be elucidated. Herein, we document immune responses among three strains of S. uberis, and preliminary explore whether and how intestinal immunity plays a role in host antiinfection processes. Mice have been proved to be effective experimental animals for bovine mastitis, so utilizing a mouse intramammary infection model, we assay immune responses and gut flora changes of three S. uberis strains by histopathologic examination, RT-PCR, Western blot, and 16s ribosomal DNA sequencing. We find that the immune responses among the three sequence-type (ST) S. uberis strains may be linked to the hasA/B and lbp virulence genes, and the beta diversity of the intestine may be independent of the ST of S. uberis. Twenty phyla and 30 genera of intestinal flora were identified, with Verrucomicrobia and Akkermansia being the most prominent phylum and genus, respectively. These bacteria have strong anti-inflammatory and protective effects against S. uberis challenge. These data provide a foundation for further studies to elucidate gut flora function and exploration of therapeutic targets for mastitis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immune response variations and intestinal flora changes in mastitis induced by three Streptococcus uberis strains

Zhou

Qiu

et al. 2022

Microbiology and Immunology

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“…Consequently, within-strain variation in the immune response to and pathogenicity of S . uberis IMI has been reported [ 9 , 10 ]. As reported in our previous study [ 11 ] and in the study of Fu, Zhou, Qiu, Chen, Zhang and Miao [ 12 ], IMI caused by S .…”

Section: Introductionmentioning

confidence: 99%

“…Environmental S. uberis strains commonly cause transient IMI, but strains showing contagious behavior can cause either transient IMI, persistent IMI, or both [5]. Consequently, within-strain variation in the immune response to and pathogenicity of S. uberis IMI has been reported [9,10]. As reported in our previous study [11] and in the study of Fu, Zhou, Qiu, Chen, Zhang and Miao [12], IMI caused by S. uberis was linked to the expression of different virulence genes, especially hasA/B and lbp.…”

Section: Introductionmentioning

confidence: 99%

Different cellular and molecular responses of Bovine milk phagocytes to persistent and transient strains of Streptococcus uberis causing mastitis

Srithanasuwan,

Schukken,

Pangprasit

et al. 2024

PLoS ONE

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Streptococcus uberis is frequently isolated from milk collected from dairy cows with mastitis. According to the host’s immunity, bacterial virulence, and their interaction, infection with some strains can induce persistent subclinical inflammation, while infection with others induces severe inflammation and transient mastitis. This study compared the inflammatory response of milk-isolated white blood cells (mWBCs) to persistent and transient S. uberis strains. Quarter milk samples were collected aseptically for bacterial culture from all lactating cows once a week over a 10-week period. A transient and noncapsular strain with a 1-week intramammary infection duration was selected from this herd, while a persistent and capsular S. uberis strain with an intramammary infection longer than 2 months from our previous study was selected based on an identical pulse field gel electrophoresis pattern during the IMI episode. Cellular and molecular responses of mWBCs were tested, and the data were analyzed using repeated analysis of variance. The results showed a higher response in migration, reactive oxygen species generation, and bacterial killing when cells were stimulated with transient S. uberis. In contrast, the persistent strain led to increased neutrophil extracellular trap release. This study also highlighted several important molecular aspects of mWBCs. Gene expression analyses by real-time RT-PCR revealed a significant elevation in the expression of Toll-like receptors (TLR-1, TLR-2, TLR-6) and proinflammatory cytokines (tumor necrosis factor-alpha or TNF-α) with the transient strain. Additionally, Streptococcus uberis capsule formation might contribute to the capability of these strains to induce different immune responses. Altogether, these results focus on the immune function of activated mWBCs which demonstrate that a transient strain can elicit a stronger local immune response and, subsequently, lead to rapid recovery from mastitis.

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Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice

Mitra

Ganaie

Bankar³

et al. 2020

Gene

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Streptococcus uberis ST439 and ST475 induce differential inflammatory responses in a mouse intramammary infection model

Cited by 5 publications

References 32 publications

Immune response variations and intestinal flora changes in mastitis induced by three Streptococcus uberis strains

Immune response variations and intestinal flora changes in mastitis induced by three Streptococcus uberis strains

Different cellular and molecular responses of Bovine milk phagocytes to persistent and transient strains of Streptococcus uberis causing mastitis

Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice

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