Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice

Mitra, Susweta Das; Ganaie, Feroze; Bankar, Kiran; Velu, Dhanikachalam; Mani, Bhuvana; Vasudevan, Madavan; Shome, Rajeswari; Rahman, Hazir; Ghosh, Sankar Kumar; Shome, Bibek Ranjan

doi:10.1016/j.gene.2019.144278

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…To evaluate the reliability of the miRNA–mRNA interaction network, the DE miRNAs and DEGs ( p ≤ 0.05 and |log 2 FC| ≥ 2) obtained in this study were compared and taken the intersections with those from previous relevant studies [ 20 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus

Wang

Fan

et al. 2021

Pathogens

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Staphylococcus aureus- induced mastitis is one of the most intractable problems for the dairy industry, which causes loss of milk yield and early slaughter of cows worldwide. Few studies have used a comprehensive approach based on the integrative analysis of miRNA and mRNA expression profiles to explore molecular mechanism in bovine mastitis caused by S. aureus. In this study, S. aureus (A1, B1 and C1) and sterile phosphate buffered saline (PBS) (A2, B2 and C2) were introduced to different udder quarters of three individual cows, and transcriptome sequencing and microarrays were utilized to detected miRNA and gene expression in mammary glands from the challenged and control groups. A total of 77 differentially expressed microRNAs (DE miRNAs) and 1625 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that multiple DEGs were enriched in significant terms and pathways associated with immunity and inflammation. Integrative analysis between DE miRNAs and DEGs proved that miR-664b, miR-23b-3p, miR-331-5p, miR-19b and miR-2431-3p were potential factors regulating the expression levels of CD14 Molecule (CD14), G protein subunit gamma 2 (GNG2), interleukin 17A (IL17A), collagen type IV alpha 1 chain (COL4A1), microtubule associated protein RP/EB family member 2 (MAPRE2), member of RAS oncogene family (RAP1B), LDOC1 regulator of NFKB signaling (LDOC1), low-density lipoprotein receptor (LDLR) and S100 calcium binding protein A9 (S100A9) in bovine mastitis caused by S. aureus. These findings could enhance the understanding of the underlying immune response in bovine mammary glands against S. aureus infection and provide a useful foundation for future application of the miRNA–mRNA-based genetic regulatory network in the breeding cows resistant to S. aureus.

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Section: Methodsmentioning

confidence: 99%

Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus

Wang

Fan

et al. 2021

Pathogens

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“…Furthermore, mastitis is one of the most prevalent diseases in dairy farming. An experiment in mammary glands of mice inoculated with Staphylococcus aureus found that 25.95% of polyploidy genes were alternatively spliced, while there were more than 100 AS events for immune-related genes . In mastitis-infected and healthy cows, a novel splice variant of the bovine lactoferrin, a member of the transferrin family (bLF) gene.…”

Section: Alternative Splicing In Plant–bacteria Interactions: Lessons...mentioning

confidence: 99%

“…An experiment in mammary glands of mice inoculated with Staphylococcus aureus found that 25.95% of polyploidy genes were alternatively spliced, while there were more than 100 AS events for immune-related genes. 46 In mastitis-infected and healthy cows, a novel splice variant of the bovine lactoferrin, a member of the transferrin family (bLF) gene. LF-AS2 has an exon-hopping AS pattern and is found only in S. aureus-infected mammary glands.…”

Section: Function Of Alternative Splicing In Bacteria-caused Animal D...mentioning

confidence: 99%

Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection

Xie,

Zhou,

Jia

et al. 2023

J. Agric. Food Chem.

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Disease represents a major problem in sustainable agricultural development. Plants interact closely with various microorganisms during their development and in response to the prevailing environment. In particular, pathogenic microorganisms can cause plant diseases, affecting the fertility, yield, and longevity of plants. During the long coevolution of plants and their pathogens, plants have evolved both molecular pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) signaling networks in order to regulate host cells in response to pathogen infestation. Additionally, in the postgenomic era, alternative splicing (AS) has become uncovered as one of the major drivers of proteome diversity, and abnormal RNA splicing is closely associated with bacterial infections. Currently, the complexity of host−bacteria interactions is a much studied area of research that has shown steady progress over the past decade. Although the development of high-throughput sequencing technologies and their application in transcriptomes have revolutionized our understanding of AS, many mechanisms related to host−bacteria interactions remain still unclear. To this end, this review summarizes the changes observed in AS during host−bacteria interactions and outlines potential therapeutics for bacterial diseases based on existing studies. In doing so, we hope to provide guidelines for plant disease management in agriculture.

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“…Studies on mRNAs have been exploring wide aspects of the pathobiology of significant viruses such as avian influenza viruses in chickens ( 4 , 5 ) and bovine viral diarrhea in bovines ( 6 ), zoonotic parasites such as toxoplasma ( 7 ) and cryptosporidium ( 8 ), and economically important pathogens that cause mastitis in bovines or caprine ( 9 , 10 ). In virtue of small RNA sequencing, similar studies are being conducted at a limited scale on miRNAs and other sncRNAs.…”

Section: Rnas In Veterinary Infectious Diseases: Where We Are: Reflec...mentioning

confidence: 99%

Editorial: Host response to veterinary infectious diseases: role of coding and non-coding RNAs as biomarkers and disease modulators

Samir

2023

Front. Vet. Sci.

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Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice

Cited by 6 publications

References 38 publications

Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus

Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus

Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection

Editorial: Host response to veterinary infectious diseases: role of coding and non-coding RNAs as biomarkers and disease modulators

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