Streptococcus troglodytae sp. nov., from the chimpanzee oral cavity

Abstract

Six strains, TKU 25, TKU 28, TKU 30, TKU 31 T , TKU 33 and TKU 34, were isolated from the oral cavity of a chimpanzee (Pan troglodytes). Colonies of strains grown on Mitis-Salivarius agar were similar in morphology to that of Streptococcus mutans. The novel strains were Gram-stainpositive, facultatively anaerobic cocci that lacked catalase activity. Analysis of the partial 16S rRNA gene sequences of these isolates showed that the most closely related strain was the type strain of S. mutans (96.4 %). The next closely related strains to the isolates were the type strains of Streptococcus devriesei (94.5 %) and Streptococcus downei (93.9 %). These isolates could be distinguished from S. mutans by inulin fermentation and alkaline phosphatase activity (API ZYM system). The peptidoglycan type of the novel isolates was Glu-Lys-Ala 3 . Strains were not susceptible to bacitracin. On the basis of phenotypic characterization, partial 16S rRNA gene and two housekeeping gene (groEL and sodA) sequence data, we propose a novel taxon, Streptococcus troglodytae sp. nov.; the type strain is TKU 31 T (5JCM 18038 T 5DSM 25324 T ).

Streptococcus pantholopis sp. nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii)

“…Growth occurs at 22, 30, 37 and 42 C but not at 4 or 15 C. Growth occurs in the presence of 3.5 % (w/v) NaCl but not in broth containing 4.5 or 6.5 % (w/v) NaCl. Acid is produced from D-galactose, D-glucose, D-fructose, D-mannose, mannitol, sorbitol, Nacetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, sucrose, trehalose, gentiobiose and D-tagatose, but not from glycerol, erythritol, D-arabinose, (Whiley & Beighton, 1998); 8, S. troglodytae TKU 31 T (Okamoto et al, 2013); 9, S. mutans NCTC 10449 T (Whiley & Beighton, 1998); 10, S. downei NCTC 11391 T (Whiley & Beighton, 1998); 11, S. dentirousetti DSM 18963 T (Takada & Hirasawa, 2008); 12, S. sobrinus NCTC 12277 T (Whiley & Beighton, 1998). All taxa gave a positive reaction in the Voges-Proskauer test and were positive for fermentation of maltose, mannitol and trehalose.…”

First report of human infection due to Streptococcus devriesei

“…devriesei , which is a transient inhabitant of the oral cavity of horses and ponies [3], [4], [8], has been shown to be strongly associated with caries in Equidae [8]. Our report shows for the first time that S. devriesei can also be responsible for human infection.…”

“…Phylogenetic analysis based on 16S rRNA gene sequences showed that S. devriesei belongs to the mutans streptococci group [3], [4]. Streptococcus mutans , which is the type species of this group, is today considered to play an important role in the development of dental caries in humans [5] and can cause endocarditis in patient at risk [6].…”

Distribution of Streptococcus troglodytae and Streptococcus dentirousetti in chimpanzee oral cavities

“…Mutans streptococci were isolated from six chimpanzees (55%). The predominant species of mutans streptococci were S. troglodytae , which has been proposed as a novel species by us (seven isolates from four chimpanzees), and S. dentirousetti (six isolates from three chimpanzees). Both S. troglodytae and S. dentirousetti were simultaneously isolated from one chimpanzee (Cleo).…”