Streptococcus plasmid pAM alpha 1 is a composite of two separable replicons, one of which is closely related to Bacillus plasmid pBC16

Perkins, John B.; Youngman, Philip

doi:10.1128/jb.155.2.607-615.1983

Cited by 77 publications

(20 citation statements)

References 27 publications

(30 reference statements)

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“…The size of region III plus the size of region I was 4.82 + 0.36 kb, which is close to the size of pDL316 (4.7 kb) estimated from the TaqI digests by using a HaeIII-digested 4X174 size marker. The size of region III plus the size of region II was 4.6 ± 0.31 kb, which is virtually identical to the reported size of pAMa1o1 (18).…”

Section: Plasmid Pamaoaj Contains Five Taqi Cleavage Sites Whichsupporting

confidence: 84%

“…The faster-migrating fragment appeared to be the same size as the EcoRI fragment H of pJH1. A fragment of this size was also present in EcoRI-digested pAMoqA1 (lane 2), a small, 4.6-kb tetracycline resistance plasmid described recently by Perkins and Youngman (18). The larger EcoRI fragment from pDL316 was not correlated with any of the fragments from pJH1 and was slightly larger than the corresponding fragment from pAMaAj1.…”

Section: Resultssupporting

confidence: 51%

“…In this paper we provide evidence that a small, 4.7-kb DNA molecule obtained from a strain of Streptococcus sanguis following transformation with a hybrid plasmid composed of DNA sequences from pJH1 and pJH2 (a hemolysin-bacteriocin plasmid also present in strain JH1 [9]) is capable of autonomous replication and expression of tetracycline resistance. This plasmid, pDL316, consisting entirely of pJH1-derived DNA, is nearly identical to pAMO1A1, a derivative of pAMot described recently by Perkins and Youngman (18)…”

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confidence: 97%

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Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon

Banai¹,

Gonda²,

Ranhand³

et al. 1985

J Bacteriol

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Streptococcus faecalis R plasmid pJHl did not transform competent strains of Streptococcus sanguis. A hybrid plasmid, pDL310, consisting of virtually all of the S. faecalis hemolysin-bacteriocin plasmid pJH2 and a segment of pJHl DNA that included the tetracycline resistance determinant, yielded tetracycline-resistant transformants at a frequency of less than 10-8 transformants per CFU, when it was added to a competent culture of S. sanguis Wicky. Four of the transformants contained a 4.7-kllobase plasmid (pDL316) that transformed strain Wicky at a frequency of 8.6 x 10-8. Restriction endonuclease digests, agarose gel electrophoresis, and Southern blot hybridizations indicated that pDL316 consisted entirely of pJHl-derived DNA. Additional restriction studies, Southern blot hybridizations, and heteroduplex analyses indicated that pDL316 was very closely related to 4.6-kilobase tetracycline resistance plasmid pAMa1Aj, a derivative of 9.0-kilobase S. faecalis plasmid pAMalpha 1.Conjugative multiple-antibiotic-resistance plasmid pJH1 from Streptococcus faecalis var. zymogenes strain JH1 (10) mediates resistance to kanamycin, streptomycin, tetracycline, and the MLS group of antibiotics (macrolides, lincosamides, and the streptogramin B type of antibiotics). Recently, we constructed a detailed restriction endonuclease map of pJH1 and located the kanamycin, streptomycin, and MLS resistance determinants within a 15-kilobase (kb) EcoRI fragment on the map of this 80 kb molecule (2). The MLS resistance determinant of pJH1 was shown to be 'on transposon Tn3871 (3), which is very similar, if not identical, to Tn917, an MLS resistance transposon originally found on pAD2, an R plasmid from S. faecalis DS16 (7,26).The tetracycline resistance determinant of pJH1, which is located several kilobases away from the kanamycin, streptomycin, and MLS resistance determinants, has been shown to share DNA sequence homology with the corresponding determinant of pAMalpha 1 (2, 4). This latter plasmid is a 9-kb nonconjugative tetracycline resistance plasmid originally described in S. faecalis DS5 (5). Furthermore, the on July 16, 2020 by guest

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Section: Plasmid Pamaoaj Contains Five Taqi Cleavage Sites Whichsupporting

confidence: 84%

Section: Resultssupporting

confidence: 51%

mentioning

confidence: 97%

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Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon

Banai¹,

Gonda²,

Ranhand³

et al. 1985

J Bacteriol

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“…2). These direct GGGC repeats and the multiple inverted, more or less perfect repeats including part of the GGGC sequences may be subject to site-specific breakage and joining, making this fragment available as a cassette to pick up antibiotic resistance genes and thus to trigger their transfer between gram-positive species via conjugative mobilization and/or interplasmid recombination (10,18,(26)(27)(28). The RepB and pIP1714 Pre(Mob) proteins are similar to several other plasmid-encoded proteins present in the data bank, but the corresponding genes are not necessarily linked on the same plasmid.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Staphylococcal Plasmid Related to pUB110 and Carrying Two Novel Genes, vatC and vgbB , Encoding Resistance to Streptogramins A and B and Similar Antibiotics

Allignet

Liassine

Solh

1998

Antimicrob Agents Chemother

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We isolated and sequenced a plasmid, named pIP1714 (4,978 bp), which specifies resistance to streptogramins A and B and the mixture of these compounds. pIP1714 was isolated from a Staphylococcus cohnii subsp. cohnii strain found in the environment of a hospital where pristinamycin was extensively used. Resistance to both compounds and related antibiotics is encoded by two novel, probably cotranscribed genes, (i) vatC, encoding a 212-amino-acid (aa) acetyltransferase that inactivates streptogramin A and that exhibits 58.2 to 69.8% aa identity with the Vat, VatB, and SatA proteins, and (ii) vgbB, encoding a 295-aa lactonase that inactivates streptogramin B and that shows 67% aa identity with the Vgb lactonase. pIP1714 includes a 2,985-bp fragment also found in two rolling-circle replication and mobilizable plasmids, pUB110 and pBC16, from gram-positive bacteria. In all three plasmids, the common fragment was delimited by two direct repeats of four nucleotides (GGGC) and included (i) putative genes closely related to repB, which encodes a replication protein, and to pre(mob), which encodes a protein required for conjugative mobilization and site-specific recombination, and (ii) sequences very similar to the double-and single-strand origins (dso, sso U ) and the recombination site, RS A . The antibiotic resistance genes repB and pre(mob) carried by each of these plasmids were found in the same transcriptional orientation.

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“…Intramolecular recombination at homologous sequences dissociates plasmid pAMctl, a tetracycline resistance plasmid of S. faecalis, into two circular molecules, each containing a single homologous sequence (16). Intermolecular recombination at homologous sequences may generate multiple-copy plasmids.…”

Section: Discussionmentioning

confidence: 99%

Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci

Neve¹,

Geis²,

Teuber³

1984

J Bacteriol

113

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Thirteen bacteriocin-producing strains of group N (lactic acid) streptococci were screened for their potential to transfer this property by conjugation to Streptococcus lactis subsp. diacetylactis Bacteriocin production in three strains was plasmid encoded as shown by conjugal transfer and by analysis of cured, bacteriocin-tiegative derivatives of the donor strains and the transconjugants. With Streptococcus cremoris strains 9B4 and 4G6 and S. lactis subsp. diacetylactis 6F7 as donors, bacteriocin-producing transconjugants were isolated with frequencies ranging from ca. 2 x 10-2 to 2 x 10-1 per recipient cell.Bacteriocin-producing transconjugants had acquired a 39.6-megadalton plasmid from the donor strains 9B4 and 4G6, and a 75-megadalton plasmid from the donor strain 6F7. As shown by restriction endonuclease analysis, the plasmids from strains 9B4 and 4G6 were almost identical. The plasmid from strain 6F7 yielded some additional fragments not present in the two other plasmids. In hybridization experiments any of the three plasmids strongly hybridized with each other and with some other bacteriocin but nontransmissible plasmids from other S. cremoris strains. Homology was also detected to a variety of cryptic plasmids in lactic acid streptococci.

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Streptococcus plasmid pAM alpha 1 is a composite of two separable replicons, one of which is closely related to Bacillus plasmid pBC16

Cited by 77 publications

References 27 publications

Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon

Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon

Characterization of a Staphylococcal Plasmid Related to pUB110 and Carrying Two Novel Genes, vatC and vgbB , Encoding Resistance to Streptogramins A and B and Similar Antibiotics

Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci

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