Streptococcal and enterococcal inhibition of endocervical lactobacilli

James, S. M.; Tagg, John

doi:10.1111/j.1574-6968.1987.tb02220.x

Cited by 7 publications

(1 citation statement)

References 15 publications

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“…The bacteriocin-like inhibitory activities of certain strains of streptococci and enterococci have been previously shown to be dependent on the presence of blood in the production and/or detection media 23,27 . The ability to produce inhibitors in blood-free, liquid cultivation media is advantageous for future physicochemical characterization assays of the inhibitor as well as its chemical purification.…”

Section: Screening For Bacteriocin Production Against Vaginal Lactobamentioning

confidence: 99%

Inhibition of Vaginal Lactobacilli by a Bacteriocin‐Like InhibitorProduced by Enterococcus faecium 62‐6: Potential Significancefor Bacterial Vaginosis

Kelly¹,

Mequio²,

Pybus³

2003

Infectious Diseases in Obstetrics and Gynecology

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Objective: Bacterial vaginosis (BV) is characterized by a shift in vaginal tract ecology, which includes a decrease in the concentration and/or prevalence of facultative lactobacilli. Currently, mechanisms which could account for the disappearance of lactobacilli are not well understood. The objective of this study was to determine whether vaginal streptococci/enterococci can produce bacteriocin-like inhibitors antagonistic to vaginal lactobacilli. Methods: Seventy strains of vaginal streptococci or enterococci were tested for antagonistic activities against vaginal lactobacilli using the deferred antagonism technique. Results: One strain, Enterococcus faecium 62-6, which strongly inhibited growth of lactobacilli was selected for further characterization. The spectrum of inhibitory activity of strain 62-6 included Gram-positive organisms from the vaginal environment, although native lactobacilli from the same host were resistant to inhibitor action. Following growth in MRS broth the strain 62-6 inhibitor was shown to be heat-(100°C, 30 minutes), cold-(4°C, less than 114 days) and pH-(4-7) stable. The sensitivity of inhibitor-containing supernatants to pepsin and α-chymotrypsin suggested an essential proteinaceous component. The inhibitor was sensitive to lipase but resistant to lysozyme. Dialysis of inhibitor-containing culture supernatants suggested a molecular mass greater than 12 000 Da. All physicochemical properties were consistent with its classification as a bacteriocin-like inhibitor. Kinetic assays demonstrated a sharp onset of inhibitor production coinciding with a concentration of 62-6 of 10 7 cfu/ml, suggesting that production may be regulated by quorum sensing. Conclusions: These results may have clinical significance as a novel mechanism to account for the decline of vaginal Lactobacillus populations and contribute to both the establishment and recurrence of BV.

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Section: Screening For Bacteriocin Production Against Vaginal Lactobamentioning

confidence: 99%

Inhibition of Vaginal Lactobacilli by a Bacteriocin‐Like InhibitorProduced by Enterococcus faecium 62‐6: Potential Significancefor Bacterial Vaginosis

Kelly¹,

Mequio²,

Pybus³

2003

Infectious Diseases in Obstetrics and Gynecology

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Lantibiotic-mediated anti-lactobacillus activity of a vaginal Staphylococcus aureus isolate

Scott

Sahl

Carne

et al. 1992

FEMS Microbiology Letters

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Staphylococcus aureus strain 26 inhibited the growth of 23 of 26 lactobacilli of endocervical origin, but only two of 17 staphylococci, in deferred antagonism tests. The inhibitory agent, a bacteriocin-like inhibitory substance (BLIS) named staphylococcin Au-26, was obtained from vigorously shaken liquid cultures containing a 0.1% (v/v) supplement of Tween 80 and was purified by chromatographic fractionation on XAD-2, carboxymethyl Sephadex and reversed phase HPLC. The molecular mass of staphylococcin Au-26 was estimated by SDS-PAGE to be approx. 2700. The detection of lanthionine residues in the molecule, the high stability to heating at acidic but not alkaline pH values and inactivation by proteinases indicate that staphylococcin Au-26 is a member of the lantibiotic class of peptide antibiotics--the first reported to be produced by a S. aureus strain. Primary sequence analysis showed that the N-terminus of the molecule is isoleucine, a characteristic also displayed by the lantibiotics nisin, epidermin and gallidermin.

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Purification and characterization of enterocin 62-6, a two-peptide bacteriocin produced by a vaginal strain ofEnterococcus faecium: Potential significance in bacterial vaginosis

DeZwaan

Mequio

Littell

et al. 2007

Microbial Ecology in Health and Disease

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A bacteriocin produced by a vaginal isolate of Enterococcus faecium strain 62-6, designated enterocin 62-6, was characterized following purification and DNA sequence analysis and compared to previously described bacteriocins. Enterocin 62-6 was isolated from brain heart infusion (BHI) culture supernatants using ammonium sulfate precipitation followed by elution from a Sepharose cation exchange column using a continuous salt gradient (0.1-0.7 M NaCl). SDS-PAGE of an active column fraction resulted in an electrophoretically pure protein, which corresponded to the growth inhibition of the sensitive Lactobacillus indicator strain in the gel overlay assay. Purified enterocin 62-6 was shown to be heat- and pH-stable, and sensitive to the proteolytic enzymes alpha-chymotrypsin and pepsin. Results from mass spectrometry suggested that it comprised two peptides of 5206 and 5219+/-1 Da, which was confirmed by DNA sequence analysis. The characteristics of enterocin 62-6 as a small, heat- and pH-stable, cationic, hydrophobic, two-peptide, plasmid-borne bacteriocin, with an inhibitory spectrum against a broad range of Gram-positive but not Gram-negative bacteria, were consistent with its classification as a class IIc bacteriocin. Furthermore, its wide spectrum of growth inhibitory activity against Gram-positive bacteria of vaginal origin including lactobacilli, and stability under the acidic conditions of the vagina, are consistent with our hypothesis that it could have potential significance in disrupting the ecology of the vaginal tract and pave the way for the establishment of the abnormal microbiota associated with the vaginal syndrome bacterial vaginosis. This is the first class IIc bacteriocin produced by a strain of E. faecium of vaginal origin to be characterized.

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Streptococcal and enterococcal inhibition of endocervical lactobacilli

Cited by 7 publications

References 15 publications

Inhibition of Vaginal Lactobacilli by a Bacteriocin‐Like InhibitorProduced by Enterococcus faecium 62‐6: Potential Significancefor Bacterial Vaginosis

Inhibition of Vaginal Lactobacilli by a Bacteriocin‐Like InhibitorProduced by Enterococcus faecium 62‐6: Potential Significancefor Bacterial Vaginosis

Lantibiotic-mediated anti-lactobacillus activity of a vaginal Staphylococcus aureus isolate

Purification and characterization of enterocin 62-6, a two-peptide bacteriocin produced by a vaginal strain ofEnterococcus faecium: Potential significance in bacterial vaginosis

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