Streptavidin-Saporin: Converting Biotinylated Materials into Targeted Toxins

Abstract

Streptavidin-Saporin can be considered a type of ‘secondary’ targeted toxin. The scientific community has taken advantage of this conjugate in clever and fruitful ways using many kinds of biotinylated targeting agents to send saporin into a cell selected for elimination. Saporin is a ribosome-inactivating protein that causes inhibition of protein synthesis and cell death when delivered inside a cell. Streptavidin-Saporin, mixed with biotinylated molecules to cell surface markers, results in powerful conjugates that are used both in vitro and in vivo for behavior and disease research. Streptavidin-Saporin harnesses the ‘Molecular Surgery’ capability of saporin, creating a modular arsenal of targeted toxins used in applications ranging from the screening of potential therapeutics to behavioral studies and animal models. The reagent has become a well-published and validated resource in academia and industry. The ease of use and diverse functionality of Streptavidin-Saporin continues to have a significant impact on the life science industry.

Streptavidin-drug conjugates streamline optimization of antibody-based conditioning for hematopoietic stem cell transplantation

“…Strategies utilizing biotin-SAv coupling for ADC screening and production have been described previously but have limitations which we sought to address via development of our SAv-drug conjugate platform. Commercially available SAv-saporin conjugates, for example, were instrumental to our demonstration that high level donor engraftment could be achieved across immunological barriers without chemotherapy or irradiation-based conditioning 17 . However, we did not observe significant antitumor benefit of CD45-saporin as a single agent in murine lymphoma or AML models 12,18 .…”

“…For preclinical modeling in the mouse, we and others have utilized the ribosome inactivating protein saporin as a toxic ADC payload 7,8,12,[14][15][16] . Saporin is commercially available in a streptavidin (SAv)conjugated format, enabling rapid, reliable production of ADCs from any biotinylated antibody 17 . However, our HSCT studies with saporin-based CD45-and cKit-ADCs showed that these conjugates behaved as nonmyeloablative conditioning agents which failed to control tumor burden in the murine A20 lymphoma model 12 .…”

Striatal parvalbumin interneurons are activated in a mouse model of cerebellar dystonia

“…We used Streptavidin-ZAP (KIT-27-Arb100, Advanced Targeting Systems, Carlsbad, CA, USA), which is a conjugate between saporin and streptavidin. Streptavidin can bind biotinylated antibodies, and when the complex binds the target cells, conjugated saporin induces cell death ( Ancheta et al, 2023 ). Streptavidin-ZAP was mixed with an anti-PV antibody (ab181086, Abcam, Cambridge, UK), which was biotinylated with a biotinylation kit (LK03, DOJINDO) in equimolar concentrations.…”

“…KIT-27-Arb100, Advanced Targeting Systems, Carlsbad, CA), which is a conjugate between saporin and streptavidin. Streptavidin can bind biotinylated antibodies, and when the complex binds the target cells, conjugated saporin induces cell death (Ancheta et al, 2023).…”