2016
DOI: 10.1002/elan.201600139
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Streptavidin‐polypyrrole Film as Platform for Biotinylated Redox Probe Immobilization for Electrochemical Immunosensor Application

Abstract: 1IntroductionElectrochemical immunosensors have proved to be promising devices in several fields such as clinical [1],p harmaceuticala pplications and environmental control [ 2]. Indeed,t hey had many advantages,s uch as robustness, low cost, high sensitivity and portability.T heir construction and use are very simple and allow detection of as mall scale (low volume, concentration, small surface …). Immunosensors are generally constructed by initially antibodies immobilization on an electrode surface,w hich is… Show more

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Cited by 4 publications
(3 citation statements)
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“…A platform for the construction of multi-array electrochemical immunosensors was prepared by immobilization of biotinylated viologen on modified polypyrrole-streptavidin films. In this strategy, the change in the electrochemical response of the redox molecule corresponding to the two electrons reduction of di-cationic to neutral viologen was monitored after the antibody-antigen interaction [Miodek et al, 2016]. The direct impedimetric detection of an anti-cholera toxin antibody was performed by using electropolymerized biotinylated poly(pyrrole-viologen) film as scaffold for the development of reagentless immunosensors [Gondram et al, 2010].…”
Section: Introductionmentioning
confidence: 99%
“…A platform for the construction of multi-array electrochemical immunosensors was prepared by immobilization of biotinylated viologen on modified polypyrrole-streptavidin films. In this strategy, the change in the electrochemical response of the redox molecule corresponding to the two electrons reduction of di-cationic to neutral viologen was monitored after the antibody-antigen interaction [Miodek et al, 2016]. The direct impedimetric detection of an anti-cholera toxin antibody was performed by using electropolymerized biotinylated poly(pyrrole-viologen) film as scaffold for the development of reagentless immunosensors [Gondram et al, 2010].…”
Section: Introductionmentioning
confidence: 99%
“…Conducting polymers (CPs) with π-conjugated backbones have been widely used for enhancing the detection of AA, DA or UA thanks to their remarkable electronic properties [16,17]. Electroactive polymers integrating redox system demonstrate the catalytic effect that enhances the sensitivity toward AA, DA and UA detection [18,19]. Electrode-modified graphene have been also demonstrated for simultaneous detection of AA, DA and UA [20,21].…”
Section: Introductionmentioning
confidence: 99%
“…Electrochemical sensors can fill that gap due to their advantages of simple operation, low cost, high sensitivity, and efficiency. The highly specific recognition between antigens and antibodies also confers high selectivity among the diverse microbiome of the upper respiratory tract. Electrochemical immunosensors determine the presence of antigens by detecting the changes in current, capacitance, potential, impedance, or conductance resultant from immunoreactions. After the primary immobilized antibody (Ab) captures the antigen (Atg), a secondary enzyme-labeled Ab is injected onto the electrode to form a sandwich assay. , The enzymatic reaction between the enzyme and enzymatic substrate results in the production of an electroactive molecule, which transmits an electrochemical signal . Alternatively, nonenzymatic-labeled-antibody combined electroactive tag could generate a large amount of redox markers to achieve electrochemical sensing, as shown in Figure a.…”
mentioning
confidence: 99%