Streptavidin‐coated surfaces suppress bacterial colonization by inhibiting non‐specific protein adsorption

Abstract

Streptavidin is a 58 kDa tetrameric protein with the highest known affinity to biotin with a wide range of applications in bionanotechnology and molecular biology. Dissolved streptavidin is stable at a broad range of temperature, pH, proteolytic enzymes and exhibits low non-specific binding. In this study, a streptavidin monolayer was assembled directly on a biotinylated TiO -surface to investigate its stability against proteolytic digestion and its suppression of initial bacterial adsorption of Escherichia coli, Bacillus subtilis, and Streptococcus intermedius. In contrast to nonmodified TiO surfaces, streptavidin-coated substrates showed only a negligible non-specific protein adsorption at physiological protein concentrations as well as a significantly reduced bacterial adhesion. The antiadhesive properties were demonstrated to be the main reason for the suppression of bacterial adhesion, which makes this approach a promising option for future surface biofunctionalization applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 758-768, 2018.

Enhanced selective cellular proliferation by multi‐biofunctionalization of medical implant surfaces with heterodimeric BMP‐2/6, fibronectin, and FGF‐2

“…SPR analysis of the streptavidin layer showed about 80% surface coverage with only minor space between the streptavidin islands. Dense coverage with streptavidin is necessary to provide sufficient and homogeneous binding epitopes for biotinylated proteins as well as for the suppression of non‐specific protein adsorption …”

“…As we also have reported earlier, a streptavidin monolayer adsorbed onto a biotinylated TiO 2 surface acts as a protein‐repellent coating. Even the adherence of bacteria is suppressed, which would prevent a localized periimplantitis . Thus, non‐specific adsorption is prevented, which is crucial for the selective biofunctionalization of an implant surface.…”

Non-Specific Adsorption Reduction Methods in Biosensing

“…Non-specific adsorption (NSA), also known as non-specific binding or biofouling is a persistent challenge in biosensing [5,8,25,26,27]. While NSA is an issue in other biological fields including implantable biomedical devices [28,29,30], and marine equipment [31,32], this report will focus on NSA for biosensing applications. Most biomolecular surfaces, whether comprised of antibodies, enzymes or proteins, experience the common issue of hindrance from non-specific species [25].…”

Polymer Domains Control Diffusion in Protein–Polymer Conjugate Biosensors

“…17,18 To minimize the effects of nonspecific binding, some techniques that make use of the weaker interactions of nonspecifically bound proteins to better select for the desired analyte, including molecular imprinting 19 and hypersonic resonance, 20 have been developed. Other methods restrict diffusion of undesired molecules to the sensor surface using protein-coated surfaces, 21 pores that enable size-based diffusion into the device, 22 or cross-linked gel coatings that greatly restrict diffusion to the sensor surface. 23 Because these latter approaches require differential diffusivities between the analyte and other species, controlling transport within surfacebased biosensors is critical to their performance.…”

“…Most detection fluids (e.g., blood and urine) primarily contain off-target molecules that block binding sites and bind to unfunctionalized surfaces of the sensors, even in diluted samples. , As such, the background signal is enhanced and/or the binding signal is reduced, resulting in decreases of limit of detection up to several orders of magnitude. , To minimize the effects of nonspecific binding, some techniques that make use of the weaker interactions of nonspecifically bound proteins to better select for the desired analyte, including molecular imprinting and hypersonic resonance, have been developed. Other methods restrict diffusion of undesired molecules to the sensor surface using protein-coated surfaces, pores that enable size-based diffusion into the device, or cross-linked gel coatings that greatly restrict diffusion to the sensor surface . Because these latter approaches require differential diffusivities between the analyte and other species, controlling transport within surface-based biosensors is critical to their performance.…”