Enhanced selective cellular proliferation by multi‐biofunctionalization of medical implant surfaces with heterodimeric BMP‐2/6, fibronectin, and FGF‐2

Abstract

Increasing cell adhesion on implant surfaces is an issue of high biomedical importance. Early colonization with endogenous cells reduces the risk of bacterial contamination and enhances the integration of an implant into the diverse cellular tissues surrounding it. In vivo integration of implants is controlled by a complex spatial and temporal interplay of cytokines and adhesive molecules. The concept of a multibiofunctionalized TiO 2 surface for stimulating bone and soft tissue growth is presented here. All supramolecular architectures were built with a biotin-streptavidin coupling system. Biofunctionalization of TiO 2 with immobilized FGF-2 and heparin could be shown to selectively increase the proliferation of fibroblasts while immobilized BMP-2 only stimulated the growth of osteoblasts. Furthermore, TiO 2 surfaces biofunctionalized with either the BMP-2 or BMP-2/6 growth factor and the cell adhesionenhancing protein fibronectin showed higher osteoblast adhesion than a TiO 2 surface functionalized with only one of these proteins. In conclusion, the presented immobilization strategy is applicable in vivo for a selective surface coating of implants in both hard and connective tissue. The combined immobilization of different extracellular proteins on implants has the potential to further influence cell-specific reactions.How to cite this article: Ettelt V, Belitsky A, Lehnert M, Loidl-Stahlhofen A, Epple M, Veith M. 2018. Enhanced selective cellular proliferation by multi-biofunctionalization of medical implant surfaces with heterodimeric BMP-2/6, fibronectin, and FGF-2.

Uncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing

“…S11D). Particularly, bFGF is known to induce proliferation of fibroblasts and SMCs [ 20 , 36 ] and to interplay with the cytokine IL1B in many infectious and inflammatory diseases [ 31 , 59 ]. EV secretion processes represent cell–cell communication and are also important for cytokine exchange [ 21 ].…”

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

“…It was shown by different groups that cell behavior is directly connected to their environment, meaning that culturing cells in 2D or 3D substrates makes an enormous difference [287]. Two-dimensional substrates, such as the classical polystyrene culture dishes, are, from a mechanobiological point of view, relatively simple, as they are easily characterized by stiffness (Young's modulus), the material's physiochemical properties (composition), and ligand density in the case of biofunctionalized materials [288,289]. Contrary to that, 3D materials offer adhesion points in all three dimensions and further show differences in porosity, microarchitecture, and local rigidity.…”

The Body’s Cellular and Molecular Response to Protein-Coated Medical Device Implants: A Review Focused on Fibronectin and BMP Proteins

“…Ettelt et al used a biotin-streptavidin multilayer system to immobilize BMP-2/6 heterodimer with fibronectin. The system prevents the side effect from growth factor over-dosage and demonstrates enhanced selective osteoblast proliferation [94].…”