Strawberry vein banding virus P6 protein intracellular transport and an important domain identification

Pan, Yuan; Zhou, Xiuhong; Li, Shuai; Feng, Ming-feng; Shi, Man-Ling; Zuo, Dengpan; Jiang, Xizi; Chen, Jing; Hu, Yahui; Zhang, Xiangxiang; Jiang, Tong

doi:10.1016/s2095-3119(18)61978-1

Cited by 2 publications

(4 citation statements)

References 43 publications

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“…For the investigation of the function of SVBV-encoded proteins, a series of plant expression vectors were constructed. Sources of SVBV US isolate (SVBV-US), China isolate (SVBV-SY), Cauliflower mosaic virus (CaMV), SVBV-US infectious clone (pSVBV-US), and expression vectors pCAM-P19, pCAM-2b, pCAM-P1, pCAM-P2, pCAM-P3, pCAM-P4, pCAM-P5, pCAM-P6, and pCAM-P6-GFP were reported previously [3,11,12]. To determine the interaction between SVBV P6 and FveIF3g, the full length of the eukaryotic cell translation initiation factor gene ( FveIF3g ) was PCR amplified from a cDNA that was taken from a total RNA sample isolated from strawberry ( F. Vesca ) leaf tissues.…”

Section: Methodsmentioning

confidence: 99%

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Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

Jiang

et al. 2018

Viruses

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The strawberry vein banding virus (SVBV) open reading frame (ORF) VI encodes a P6 protein known as the RNA silencing suppressor. This protein is known to form inclusion like granules of various sizes and accumulate in both the nuclei and the cytoplasm of SVBV-infected plant cells. In this study, we have determined that the P6 protein is the only trans-activator (TAV) encoded by SVBV, and can efficiently trans-activate the translation of downstream gfp mRNA in a bicistron derived from the SVBV. Furthermore, the P6 protein can trans-activate the expression of different bicistrons expressed by different caulimovirus promoters. The P6 protein encoded by SVBV from an infectious clone can also trans-activate the expression of bicistron. Through protein-protein interaction assays, we determined that the P6 protein could interact with the cell translation initiation factor FveIF3g of Fragaria vesca and co-localize with it in the nuclei of Nicotiana benthamiana cells. This interaction reduced the formation of P6 granules in cells and its trans-activation activity on translation.

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Section: Methodsmentioning

confidence: 99%

“…The CaMV ORF VI-encoded P6 protein was reported as a multifunctional protein [10]. For example, CaMV P6 can function as an RNA silencing suppressor, and is the main component of CaMV inclusion bodies [11,12]. It is also known to be a disease symptom and host range determinant [13].…”

Section: Introductionmentioning

confidence: 99%

Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

Jiang

et al. 2018

Viruses

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“…The strawberry vein banding virus (SVBV) is a latent virus that severely harms strawberry plants in major strawberry growing areas worldwide ( Ratti et al, 2009 ; Chen et al, 2016a ). In China, SVBV, which is primarily transmitted by aphids or tissue culture seedlings, is mainly distributed in the Sichuan, Hebei, Liaoning, Shandong, and Anhui provinces ( Pan et al, 2018 ; Jiang et al, 2021 ), where SVBV-infected strawberries display characteristic symptoms, such as weak growth, uneven leaf color, a decrease in the number of creeping stems, and deformed fruits ( Frazier, 1974 ).…”

Section: Introductionmentioning

confidence: 99%

“…SVBV shares similar genome structure with cauliflower mosaic virus (CaMV) belonging to the same genus. According to the proteins encoded by each ORF of CaMV, it was inferred that ORF II, III, IV, and V of SVBV may encode proteins associated with aphid infection, a DNA-binding protein, a coat protein (CP), a reverse transcriptase protein ( Pan et al, 2018 ). P6, encoded by ORF VI, is a multifunctional protein that functions as an RNA silencing suppressor and translation transactivator ( Li et al, 2018a ), and ORF VII encodes an unknown protein ( Schoelz et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection

Zhang²,

Xu³

et al. 2022

Front. Microbiol.

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Chlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of Fragaria vesca (FvLHC II-1L) affects viral infection remains unclear. In this study, we demonstrated that the movement protein P1 of strawberry vein banding virus (SVBV P1) interacted with FvLHC II-1L in vivo and in vitro by bimolecular fluorescence complementation and pull-down assays. SVBV P1 was co-localized with FvLHC II-1L at the edge of epidermal cells of Nicotiana benthamiana leaves, and FvLHC II-1L protein expression was upregulated in SVBV-infected F. vesca. We also found that FvLHC II-1L effectively promoted SVBV P1 to compensate for the intercellular movement of movement-deficient potato virus X (PVXΔP25) and the systemic movement of movement-deficient cucumber mosaic virus (CMVΔMP). Transient overexpression of FvLHC II-1L and inoculation of an infectious clone of SVBV showed that the course of SVBV infection in F. vesca was accelerated. Collectively, the results showed that SVBV P1 protein can interact with FvLHC II-1L protein, which in turn promotes F. vesca infection by SVBV.

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Strawberry vein banding virus P6 protein intracellular transport and an important domain identification

Cited by 2 publications

References 43 publications

Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection

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