Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

Abstract

The strawberry vein banding virus (SVBV) open reading frame (ORF) VI encodes a P6 protein known as the RNA silencing suppressor. This protein is known to form inclusion like granules of various sizes and accumulate in both the nuclei and the cytoplasm of SVBV-infected plant cells. In this study, we have determined that the P6 protein is the only trans-activator (TAV) encoded by SVBV, and can efficiently trans-activate the translation of downstream gfp mRNA in a bicistron derived from the SVBV. Furthermore, the P6 protein can trans-activate the expression of different bicistrons expressed by different caulimovirus promoters. The P6 protein encoded by SVBV from an infectious clone can also trans-activate the expression of bicistron. Through protein-protein interaction assays, we determined that the P6 protein could interact with the cell translation initiation factor FveIF3g of Fragaria vesca and co-localize with it in the nuclei of Nicotiana benthamiana cells. This interaction reduced the formation of P6 granules in cells and its trans-activation activity on translation.

Modulation of gut microbiota protects against viral respiratory tract infections: a systematic review of animal and clinical studies

“…Of these, studies regard to Lactobacillus [ 9 , 30 , 34 , 45 , 54 ], Lactococcus [ 26 , 62 ], Bifidobacterium [ 57 ], or Enterococcus [ 71 ] administration showed increased production of either IFN-α or IFN-β. Sixteen studies addressed the alteration of IFN-γ in respiratory virus infected mice administered with probiotics [ 26 , 33 , 39 , 48 , 51 , 52 , 54 – 58 , 69 , 71 – 74 ]. There are 12 studies declared the increase of IFN-γ after administration of probiotic Lactobacillus [ 30 , 32 , 33 , 39 , 48 , 51 , 52 , 54 , 55 ], Bifidobacterium [ 57 , 58 ], or Bifico [ 73 ] (a probiotic mixture consisting Bifidobacterium, Lactobacillus acidophilus , and Enterococcus ).…”

RNA‐dependent RNA polymerase 1 delays the accumulation of viroids in infected plants

“…Protein extraction and western blot hybridization were performed according to Li et al ( 2018 ). After transferring protein to nitrocellulose membranes, the membranes were probed with a mouse anti‐GFP or anti‐myc antibody and then probed with a horseradish peroxidase‐labelled goat anti‐mouse antibody.…”

Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of Fragaria vesca to Promote Viral Infection

“…The coding sequences of SVBV P1 (GenBank No: X97304.1) and FvLHC II-1L (GenBank No: XM_004303830.2) were cloned into pCV-cYFP and pCV-nYFP, respectively ( Li et al, 2018a ), to generate cYFP-SVBV P1, nYFP-FvLHC II-1L, nYFP-P1, and cYFP-FvLHC II-1L. The recombinant plasmids were transformed into A. tumefaciens (GV3101) and co-infiltrated into N. benthamiana leaves.…”

“…According to the proteins encoded by each ORF of CaMV, it was inferred that ORF II, III, IV, and V of SVBV may encode proteins associated with aphid infection, a DNA-binding protein, a coat protein (CP), a reverse transcriptase protein ( Pan et al, 2018 ). P6, encoded by ORF VI, is a multifunctional protein that functions as an RNA silencing suppressor and translation transactivator ( Li et al, 2018a ), and ORF VII encodes an unknown protein ( Schoelz et al, 2016 ). Our previous study showed that SVBV P1 may regulates intracellular and intercellular movement of SVBV during viral infection ( Rui et al, 2021 ).…”