Stratification Effects on Endogenous Gibberellic Acid (GA) in Peach Seeds1

Seed dormancy is regulated by complex networks in order to optimize the timing of germination. However, the biochemical basis of the regulation of seed dormancy is still poorly understood. Many temperate timber species, which are of ecological and/or economic interest, are deeply dormant in seeds, such as Prunus campanulata. Freshly harvested seeds require warm plus cold stratification to break dormancy before they can begin to germinate. According to the results of germination, both warm and cold stratifications are the critical influences for breaking seed dormancy. Significant variations in seed proteins were observed by 2-DE before and after the breaking of seed dormancy. Among the 320, 455, and 491 reproducibly detected spots on the cotyledons, embryos, and testae, respectively, 71 dramatic changes in abundances were observed following warm and/or cold stratification. Among these protein spots, dehydrin, prunin 1 precursor, prunin 2 precursor, and prunin 2 were identified by MS and sequence comparison. The implications of protein changes in relation to the breaking of seed dormancy and germination are discussed. This is the first report of a proteomic analysis of dormancy breaking in woody plant seeds.

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Protein changes between dormant and dormancy‐broken seeds of Prunus campanulata Maxim

Lee

Lin²,

Yang

2006

Proteomics

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Beneficial effects of cold-moist stratification on seed germination behaviors of Abies pindrow and Picea smithiana

Rawat

Khanduri

Sharma

2008

Journal of Forestry Research

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A study was conducted to evaluate the effect of GA 3 , moist-chilling and temperature on seed germination of Abies pindrow and Picea smithiana from five different provenances. Seeds were soaked in GA 3 (10 mg⋅L -1 ) for 24 h, then chilled at 3−5°C for 15 days. Four temperature regimes viz. 10°C, 15°C, 20°C and 25°C were used for stimulating seed germination. Results showed that soaking and chilling significantly increased germination percentage. The germination percentage was highest at 10°C. Overall results showed that soaking seeds in GA 3 (10 mg⋅L -1 ) for 24 h, moist chilling for 15 days, and germinating at 10°C produced an effective germination in both the species studied. The statistical analysis of the data proclaimed significant effect of treatment, temperature, provenance and treatment with temperature interactions on seed germination.

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Effects of temperature, chemical treatments and endogenous hormone content on peach seed germination and subsequent seedling growth

Mehanna

Martin

Nishijima

1985

Scientia Horticulturae

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Stratification Effects on Endogenous Gibberellic Acid (GA) in Peach Seeds1

Cited by 8 publications

References 6 publications

Protein changes between dormant and dormancy‐broken seeds of Prunus campanulata Maxim

Protein changes between dormant and dormancy‐broken seeds of Prunus campanulata Maxim

Beneficial effects of cold-moist stratification on seed germination behaviors of Abies pindrow and Picea smithiana

Effects of temperature, chemical treatments and endogenous hormone content on peach seed germination and subsequent seedling growth

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