Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development

Petersen, Rejane L.

doi:10.3390/bios7040049

Cited by 98 publications

(73 citation statements)

References 61 publications

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“…Figure S1c (Supporting Information) presents evidence of the association of label particles (PEI‐Au‐Ps) with a ∆λ of 1.168 nm (1030 s). The described results are in full agreement with recent reports characterizing aptamer‐cell and aptamer‐protein interactions demonstrating the correlation between ∆λ and the optical thickness of the sensor layer as a function of time The results provide evidence of the proposed detection mechanism by a sandwich‐like interaction between the immobilized aptamer, the associated cells and the label particles.…”

Section: Resultssupporting

confidence: 91%

Inkjet Printed Nanopatterned Aptamer‐Based Sensors for Improved Optical Detection of Foodborne Pathogens

Díaz‐Amaya

Zhao

Lin

et al. 2019

Small

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The increasing incidence of infectious outbreaks from contaminated food and water supply continues imposing a global burden for food safety, creating a market demand for on‐site, disposable, easy‐to‐use, and cost‐efficient devices. Despite of the rapid growth of biosensors field and the generation of breakthrough technologies, more than 80% of the platforms developed at lab‐scale never will get to meet the market. This work aims to provide a cost‐efficient, reliable, and repeatable approach for the detection of foodborne pathogens in real samples. For the first time an optimized inkjet printing platform is proposed taking advantage of a carefully controlled nanopatterning of novel carboxyl‐functionalized aptameric ink on a nitrocellulose substrate for the highly efficient detection of E. coli O157:H7 (25 colony forming units (CFU) mL−1 in pure culture and 233 CFU mL−1 in ground beef) demonstrating the ability to control the variation within ±1 SD for at least 75% of the data collected even at very low concentrations. From the best of the knowledge this work reports the lowest limit of detection of the state of the art for paper‐based optical detection of E. coli O157:H7, with enough evidence (p > 0.05) to prove its high specificity at genus, species, strain, and serotype level.

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Section: Resultssupporting

confidence: 91%

Inkjet Printed Nanopatterned Aptamer‐Based Sensors for Improved Optical Detection of Foodborne Pathogens

Díaz‐Amaya

Zhao

Lin

et al. 2019

Small

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“…Compared with the electrochemical method and fluorescence method, SPR has the advantages of non-labeling, high sensitivity and weight, good renaturation, low sample consumption, and wide detection range, and has a good application prospect in the fields of food safety, clinical diagnosis, drug targeting therapy [69]. A biolayer interferometry (BLI) biosensor is a biomembrane-dependent interferometric technique, this label-free and real-time monitoring technology could realize the determination of dynamic parameters of intermolecular interaction with the merits of being simple, fast, high throughput, of low cost and easily maintained [70]. It is also an effective alternative to ELISA, HPLC and SPR analytic methods.…”

Section: The Development Of Biosensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

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Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.Key Contribution: This article reviews systematically the toxicity of marine toxins, the development of aptarmer-based biosensors, and progress in the marine toxin detection by aptasensors.Toxins 2020, 12, 1 2 of 22 selection technology and biosensor fabrication technology offer a new solution for the detection of marine toxins with high efficiency and sensitivity [13][14][15][16][17].Marine toxins are a class of small molecular compounds mainly produced by red tide algae. In recent years, with the aggravation of environmental pollution, human deaths due to accidental ingestion of toxic shellfish are also often reported [18][19][20][21]. At present, the main methods for the detection of marine toxins include mouse bioassay (MBA) [22], enzyme-linked immunosorbent assay (ELISA) [23][24][25], high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), etc. [26][27][28]. However, these traditional methods have some drawbacks including the requirement of a technician, poor repeatability, expensive equipment, and issues related to animal ethics [22]. As a new detection technology, the biosensor is a kind of analysis systems using cell molecules and other bio-materials as sensitive elements, combined with a secondary sensor to detect a variety of chemicals by cascade amplified signal [29]. Because of their advantages of simple operation, high speed, high sensitivity, miniaturization, and easy automation, biosensors have been widely used in different fields including cell physiology [30], drug screening [31,32], food safety detection [33], disease diagnosis [34], etc.Aptamer, meaning "to fit", is a kind of oligonucleotide which can bind to target molecules with high selectivity and affinity. On account of the existence of aptamers in nature and the po...

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“…The binding of rWLP with actin was measured by Bio-Layer Interferometry (BLI) on an Octet RED BLI (Pall ForteBio) at 25˚C [31]. The rWLP (1 mM) was dissolved in PBS buffer (pH 7.4) containing 0.05% (v/v) Tween 20 and 0.1% (v/v) BSA and then loaded onto an APS biosensor (Pall ForteBio) coated with actin.…”

Section: Biolayer Interferometrymentioning

confidence: 99%

Molecular characterization of a whirlin-like protein with biomineralization-related functions from the shell of Mytilus coruscus

et al. 2020

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Mollusc shells are produced from calcified skeletons and have excellent mechanical properties. Shell matrix proteins (SMPs) have important functions in shell formation. A 16.6 kDa whirlin-like protein (WLP) with a PDZ domain was identified in the shell of Mytilus coruscus as a novel SMP. In this study, the expression, function, and location of WLP were analysed. The WLP gene was highly expressed and specifically located in the adductor muscle and mantle. The expression of recombinant WLP (rWLP) was associated with morphological change, polymorphic change, binding ability, and crystallization rate inhibition of the calcium carbonate crystals in vitro. In addition, an anti-rWLP antibody was prepared, and the results from immunohistochemistry and immunofluorescence analyses revealed the specific location of the WLP in the mantle, adductor muscle, and myostracum layer of the shell, suggesting multiple functions for WLP in biomineralization, muscle-shell attachment, and muscle attraction. Furthermore, results from a pull-down analysis revealed 10 protein partners of WLP in the shell matrices and a possible network of interacting WLPs in the shell. In addition, in this study, one of the WLP partners, actin, was confirmed to have the ability to bind WLP. These results expand the understanding of the functions of PDZ-domain-containing proteins in biomineralization and provide clues for determining the mechanisms of myostracum formation and muscle-shell attachment. OPEN ACCESS Citation: Jiang Y, Sun Q, Fan M, Zhang X, Shen W, Xu H, et al. (2020) Molecular characterization of a whirlin-like protein with biomineralization-related functions from the shell of Mytilus coruscus. PLoS ONE 15(4): e0231414. https://doi.org/10.

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Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development

Cited by 98 publications

References 61 publications

Inkjet Printed Nanopatterned Aptamer‐Based Sensors for Improved Optical Detection of Foodborne Pathogens

Inkjet Printed Nanopatterned Aptamer‐Based Sensors for Improved Optical Detection of Foodborne Pathogens

Marine Toxins Detection by Biosensors Based on Aptamers

Molecular characterization of a whirlin-like protein with biomineralization-related functions from the shell of Mytilus coruscus

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