Inkjet Printed Nanopatterned Aptamer‐Based Sensors for Improved Optical Detection of Foodborne Pathogens

Díaz‐Amaya, Susana; Zhao, Min; Lin, Li‐Kai; Ostos, C.; Allebach, Jan P.; Chiu, George T.‐C.; Deering, Amanda J.; Stanciu, Lia

doi:10.1002/smll.201805342

Cited by 36 publications

(25 citation statements)

References 49 publications

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“…[114] Copyright 2019, Royal Society of Chemistry); D) test-strip system proposed by Díaz-Amaya et al [115] (Reproduced with permission. [115] Copyright 2019, Wiley-VCH GmbH); E) schematic illustration of the design proposed by Zhou et al [117] (Reproduced with permission. [117] Copyright 2020, American Chemical Society).…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

“…AuNCs (20 nm) were then immobilized on the surface of polystyrene beads and coated with branched PEI. [115] A truncated aptameric DNA sequence (a-aptamer) highly specific to E. Coli O157:H7 was then conjugated to the fabricated particles. Upon addition of the bacterial sample, it was recognized and interacted with the inkjet patterned capture aptamer (carboxy/ DNA aptamer) found on the test and label lines.…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

“…The LOD obtained was 25 and 233 CFU mL −1 , when tested in PBS and ground beef respectively. [115] Even with a very LOD of 25 CFU mL −1 , it was established that this may not be as accurate owing to the low concentration and small sample volume of <100μL and high possibility of poor reproducibility. Conversely, at concentrations >100 CFU mL −1 , a linear correlation between the intensity response and concentration was observed.…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

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Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Gopal

Kashif

et al. 2021

Adv Healthcare Materials

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With an exponential rise in antimicrobial resistance and stagnant antibiotic development pipeline, there is, more than ever, a crucial need to optimize current infection therapy approaches. One of the most important stages in this process requires rapid and effective identification of pathogenic bacteria responsible for diseases. Current gold standard techniques of bacterial detection include culture methods, polymerase chain reactions, and immunoassays. However, their use is fraught with downsides with high turnaround time and low accuracy being the most prominent. This imposes great limitations on their eventual application as point-of-care devices. Over time, innovative detection techniques have been proposed and developed to curb these drawbacks. In this review, a systematic summary of a range of biosensing platforms is provided with a strong focus on technologies conferring high detection sensitivity and specificity. A thorough analysis is performed and the benefits and drawbacks of each type of biosensor are highlighted, the factors influencing their potential as point-of-care devices are discussed, and the authors' insights for their translation from proof-of-concept systems into commercial medical devices are provided.

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Section: Colorimetric Biosensorsmentioning

confidence: 99%

Section: Colorimetric Biosensorsmentioning

confidence: 99%

Section: Colorimetric Biosensorsmentioning

confidence: 99%

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Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Gopal

Kashif

et al. 2021

Adv Healthcare Materials

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“…[212,213] The most frequently used AM technique for aptamers is inkjet printing. [214,215] Aptamers exhibit low thermal stability (<100 °C), [216][217][218] which precludes their use by high-temperature AM techniques. However, there is potential to improve thermal stability by exploring new aptamer sequences.…”

Section: Aptamersmentioning

confidence: 99%

Additive Manufacturable Materials for Electrochemical Biosensor Electrodes

Elbadawi

Ong

Pollard

et al. 2020

Adv Funct Materials

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With the impending Industrial Revolution 4.0, the information produced by sensors will be central in many applications. This includes the healthcare sector, where affordable healthcare and precision medicine are highly sought after. Electrochemical sensors have the potential to produce affordable, high sensitivity and specificity, intuitive, and rapid point‐of‐care diagnostics. Underpinning these achievements is the choice of material and the fabrication thereof. In this review, the different types of materials used in electrochemical biosensors are reported, with a focus on synthetic conductive materials. The review demonstrates that there is an abundance of materials to select from, and compositing different types of materials further widens their applicability in biosensors. In addition, the fabrication of such materials using the state‐of‐the‐art of fabrication technology, additive manufacturing (AM), is also detailed. The need for compositing is evident in AM, as the feedstock for certain AM technologies is inherently nonconductive. Both material choice and fabrication technologies limitations are also discussed to highlight opportunities for growth. The review highlights how recent technological advancements have the potential to drive the healthcare industry toward achieving its primary goals.

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“…Printing techniques have been drawing growing attention in many applications such as electronics [ 1–5 ] and sensors [ 6–10 ] because of low cost, low material consumption, high scalability, opportunity of exploiting novel properties of nanomaterials, and advantage of using high throughput and additive manufacturing. However, the commonly used printing techniques, including inkjet printing, [ 11 ] gravure printing, [ 12,13 ] and screen printing, [ 14,15 ] have limited scalability and resolution (micro or sub‐micro scale).…”

Section: Figurementioning

confidence: 99%

High‐Rate Printing of Micro/Nanoscale Patterns Using Interfacial Convective Assembly

et al. 2020

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Electric-field-directed assembly [31,32] uses electrophoretic and dielectrophoretic forces to direct nanoelements. Although electric-field-directed assembly demonstrates high throughput, high scalability, and high resolution in assembling various nanomaterials, conductive substrates are required to generate electric field, which limits potential applications of printed structures in electronic devices. Fluidicflow-directed assembly such as convective [33][34][35] and capillary [36][37][38] assembly utilizes solvent evaporation-induced convective flow to guide and position nanoelements. Unlike electric-field-directed assembly, fluidic-flow-directed assembly is applicable to all kinds of substrates (both insulating and conductive). However, it takes hours to assemble over centimetersized substrates. Therefore, the scalability and throughput of this assembly process is a major challenge. Photothermally directed assembly uses photothermaleffect-induced Rayleigh-Benard [39] and/or Marangoni [40][41][42] convective flow to direct assembly. Similar to fluidic-flow-directed assembly, photothermally directed assembly suffers from scalability and throughput issues due to its serial processing nature. In addition, photothermally directed assembly has a limited resolution of micro or sub-micro scale. [43,44] Therefore, the development of a broadly applicable, highly scalable, high throughput, and high resolution printing technique is highly desirable.Here, we report a novel fluidic-flow-directed assembly technique, interfacial convective assembly, to rapidly assemble particles in pattern areas on any substrates. In the interfacial convective assembly, a patterned substrate is initially sonicated in isopropyl alcohol (IPA) using a bath type sonicator for a few seconds. With a low surface tension, IPA helps wet as well as remove trapped air in the patterned structures. After sonication, the substrate is removed from the IPA bath, leaving a thin IPA film on the substrate. Afterward, 50 µL aqueous particle suspension is drop casted on the IPA film. The particle suspension immediately mixes with the IPA film, resulting a suspension with ≈20 wt% IPA. Then, a glass slide is covered on the suspension, creating a confined environment with the substrate via a 300 µm thick spacer (Figure 1a). Finally, the setup is placed onto a hot plate with preheated temperatures between 40 and 75 °C. The substrate is heated up, inducing a convective flow. Due to the convective flow, particles are carried toward Printing of electronics has been receiving increasing attention from academia and industry over the recent years. However, commonly used printing techniques have limited resolution of micro-or sub-microscale. Here, a directed-assembly-based printing technique, interfacial convective assembly, is reported, which utilizes a substrate-heating-induced solutal Marangoni convective flow to drive particles toward patterned substrates and then uses van der Waals interactions as well as geometrical confinement to trap the particles in the pattern area...

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Inkjet Printed Nanopatterned Aptamer‐Based Sensors for Improved Optical Detection of Foodborne Pathogens

Cited by 36 publications

References 49 publications

Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Biosensors and Point‐of‐Care Devices for Bacterial Detection: Rapid Diagnostics Informing Antibiotic Therapy

Additive Manufacturable Materials for Electrochemical Biosensor Electrodes

High‐Rate Printing of Micro/Nanoscale Patterns Using Interfacial Convective Assembly

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