Strategies to promote the vascularization of skin substitutes after transplantation

Bourland, Jennifer; Fradette, Julie

doi:10.1016/b978-0-12-810545-0.00008-5

Cited by 3 publications

(4 citation statements)

References 99 publications

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“…Nevertheless, HUVECs could be replaced by human dermal microvascular endothelial cells which would be more relevant for a skin model. In fact, HUVECs are isolated from large vessels whereas dermis vasculature mainly consists of microvessels [31]. Vessel diameter is not the only difference, but this parameter could influence cell behavior as it was already shown that endothelial cells from different blood vessels or different tissues have distinct gene expression profiles [86].…”

Section: Discussionmentioning

confidence: 99%

“…Certainly, having a perfusable vascular network in skin equivalents makes more relevant the evaluation of systemic delivery of substances, but also the study of systemic exposure or inflammation responses which could not be properly assessed with existing skin models. Improving vascularization of tissue-engineered constructs has therefore gained interest in the last decade [31]. Many strategies have been developed to improve or accelerate vascular growth in engineered tissues either in vivo relying on improving the angiogenesis process from the host tissue into the implant [32][33][34][35] or by prevascularization of the engineered tissue.…”

Section: Introductionmentioning

confidence: 99%

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A perfusable vascularized full-thickness skin model for potential topical and systemic applications

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A perfusable vascularized full-thickness skin model for potential topical and systemic applications

et al. 2021

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“…To ensure the survival, the integration and the maintenance of the functions of engineered substitutes, a fast and adequate vascularization must be achieved upon grafting [1,2]. Angiogenesis can be stimulated by the use of various in vitro strategies that have been addressed in many reviews [3][4][5][6][7][8]. In particular, cells forming engineered tissues can be genetically modified to express angiogenic factors.…”

Section: Introductionmentioning

confidence: 99%

Short-term post-implantation dynamics of in vitro engineered human microvascularized adipose tissues

et al. 2018

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Engineered adipose tissues are developed for their use as substitutes for tissue replacement in reconstructive surgery. To ensure a timely perfusion of the grafted substitutes, different strategies can be used such as the incorporation of an endothelial component. In this study, we engineered human adipose tissue substitutes comprising of functional adipocytes as well as a natural extracellular matrix using the self-assembly approach, without the use of exogenous scaffolding elements. Human microvascular

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“…Furthermore, the dermal layer actively contributes to wound re-epithelialization by supporting keratinocyte adhesion, migration, and differentiation [41]. Hence, dermal regeneration is a crucial part of skin reconstruction.…”

Section: Dermal Substitutes and Skin Graftingmentioning

confidence: 99%

The Crucial Role of Vascularization and Lymphangiogenesis in Skin Reconstruction

et al. 2018

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Background: The treatment of extensive skin defects and bradytrophic wounds remains a challenge in clinical practice. Despite emerging tissue engineering approaches, skin grafts and dermal substitutes are still the routine procedure for the majority of skin defects. Here, we review the role of vascularization and lymphangiogenesis for skin grafting and dermal substitutes from the clinician’s perspective. Summary: Graft revascularization is a dynamic combination of inosculation, angiogenesis, and vasculogenesis. The majority of a graft’s microvasculature regresses and is replaced by ingrowing microvessels from the wound bed, finally resulting in a chimeric microvascular network. After inosculation within 48–72 h, the graft is re-oxygenated. In contrast to skin grafts, the vascularization of dermal substitutes is slow and dependent on the ingrowth of vessel-forming angiogenic cells. Preclinical angiogenic strategies with adipose tissue-derived isolates are appealing for the treatment of difficult wounds and may markedly accelerate skin reconstruction in the future. However, their translation from bench to bedside is still restricted by major regulatory restrictions. Finally, the lymphatic system contributes to edema reduction and the removal of local wound debris. Therapeutic lymphangiogenesis is an emerging field of research in skin reconstruction. Key Messages: For the successful engraftment of skin grafts and dermal substitutes, the rapid formation of a microvascular network is of pivotal importance. Hence, to understand the biological processes behind revascularization of skin substitutes and to implement this knowledge into clinical practice is a prerequisite when treating skin defects. Furthermore, a functional lymphatic drainage crucially contributes to the engraftment of skin substitutes.

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Strategies to promote the vascularization of skin substitutes after transplantation

Cited by 3 publications

References 99 publications

A perfusable vascularized full-thickness skin model for potential topical and systemic applications

A perfusable vascularized full-thickness skin model for potential topical and systemic applications

Short-term post-implantation dynamics of in vitro engineered human microvascularized adipose tissues

The Crucial Role of Vascularization and Lymphangiogenesis in Skin Reconstruction

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