Strategies of strengthening mechanical properties in the osteoinductive calcium phosphate bioceramics

Li, Qipeng; Feng, Cong; Cao, Quanle; Wang, Wei; Ma, Zihan; Wu, Yonghao; He, Tinghan; Jing, Yangtian; Tan, Wenxuan; Liao, Tongxiao; Xing, Jiadi; Li, Xiangfeng; Wang, Ye; Xiao, Yumei; Zhu, Xiangdong; Zhang, Xingdong

doi:10.1093/rb/rbad013

Cited by 7 publications

(2 citation statements)

References 122 publications

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“…Reinforcements by incorporating fibers, such as carbon or polymer fibers, into β-TCP scaffolds can provide additional support. Nanoengineering of β-TCP can lead to enhanced mechanical properties due to increased surface area and altered crystal structure, as exemplified in a recent review article of Li and co-authors [ 12 ]. Despite the progress made in improving the mechanical properties of β-TCP, several challenges and pitfalls remain: β-TCP is inherently brittle, which can limit its use in load-bearing applications.…”

Section: Discussionmentioning

confidence: 99%

Mechanical and Functional Improvement of β-TCP Scaffolds for Use in Bone Tissue Engineering

Umrath,

Schmitt,

Kliesch

et al. 2023

JFB

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Autologous bone transplantation is still considered as the gold standard therapeutic option for bone defect repair. The alternative tissue engineering approaches have to combine good hardiness of biomaterials whilst allowing good stem cell functionality. To become more useful for load-bearing applications, mechanical properties of calcium phosphate materials have to be improved. In the present study, we aimed to reduce the brittleness of β-tricalcium phosphate (β-TCP). For this purpose, we used three polymers (PDL-02, -02a, -04) for coatings and compared resulting mechanical and degradation properties as well as their impact on seeded periosteal stem cells. Mechanical properties of coated and uncoated β-TCP scaffolds were analyzed. In addition, degradation kinetics analyses of the polymers employed and of the polymer-coated scaffolds were performed. For bioactivity assessment, the scaffolds were seeded with jaw periosteal cells (JPCs) and cultured under untreated and osteogenic conditions. JPC adhesion/proliferation, gene and protein expression by immunofluorescent staining of embedded scaffolds were analyzed. Raman spectroscopy measurements gave an insight into material properties and cell mineralization. PDL-coated β-TCP scaffolds showed a significantly higher flexural strength in comparison to that of uncoated scaffolds. Degradation kinetics showed considerable differences in pH and electrical conductivity of the three different polymer types, while the core material β-TCP was able to stabilize pH and conductivity. Material differences seemed to have an impact on JPC proliferation and differentiation potential, as reflected by the expression of osteogenic marker genes. A homogenous cell colonialization of coated and uncoated scaffolds was detected. Most interesting from a bone engineer’s point of view, the PDL-04 coating enabled detection of cell matrix mineralization by Raman spectroscopy. This was not feasible with uncoated scaffolds, due to intercalating effects of the β-TCP material and the JPC-formed calcium phosphate. In conclusion, the use of PDL-04 coating improved the mechanical properties of the β-TCP scaffold and promoted cell adhesion and osteogenic differentiation, whilst allowing detection of cell mineralization within the ceramic core material.

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Section: Discussionmentioning

confidence: 99%

Mechanical and Functional Improvement of β-TCP Scaffolds for Use in Bone Tissue Engineering

Umrath,

Schmitt,

Kliesch

et al. 2023

JFB

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“…However, the low mechanical strength of normal HAp ceramics restricts its use mainly to low load-bearing applications [29]. To overcome this issue, we incorporated reduced graphene oxide (rGO) nanosheets into HAp to increase the mechanical strength and osteogenic capability.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide

Kang

Park

et al. 2023

Cells

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In recent years, bone tissue engineering (BTE) has made significant progress in promoting the direct and functional connection between bone and graft, including osseointegration and osteoconduction, to facilitate the healing of damaged bone tissues. Herein, we introduce a new, environmentally friendly, and cost-effective method for synthesizing reduced graphene oxide (rGO) and hydroxyapatite (HAp). The method uses epigallocatechin-3-O-gallate (EGCG) as a reducing agent to synthesize rGO (E-rGO), and HAp powder is obtained from Atlantic bluefin tuna (Thunnus thynnus). The physicochemical analysis indicated that the E-rGO/HAp composites had exceptional properties for use as BTE scaffolds, as well as high purity. Moreover, we discovered that E-rGO/HAp composites facilitated not only the proliferation, but also early and late osteogenic differentiation of human mesenchymal stem cells (hMSCs). Our work suggests that E-rGO/HAp composites may play a significant role in promoting the spontaneous osteogenic differentiation of hMSCs, and we envision that E-rGO/HAp composites could serve as promising candidates for BTE scaffolds, stem-cell differentiation stimulators, and implantable device components because of their biocompatible and bioactive properties. Overall, we suggest a new approach for developing cost-effective and environmentally friendly E-rGO/HAp composite materials for BTE application.

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Phase transformations during the dissolution-precipitation synthesis of magnesium whitlockite nanopowders from gypsum

Afonina,

Dubauskas,

Klimavicius

et al. 2023

Ceramics International

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Strategies of strengthening mechanical properties in the osteoinductive calcium phosphate bioceramics

Cited by 7 publications

References 122 publications

Mechanical and Functional Improvement of β-TCP Scaffolds for Use in Bone Tissue Engineering

Mechanical and Functional Improvement of β-TCP Scaffolds for Use in Bone Tissue Engineering

Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide

Phase transformations during the dissolution-precipitation synthesis of magnesium whitlockite nanopowders from gypsum

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