Strategies for hepatic support in acute liver failure: Role of extracorporeal devices versus liver transplantation

Groot, Gerrit H. de; Schalm, Solko W.; Fick, T E; Reuvers, Cees B.; Boks, Anton L.; Terpstra, Onno T.

doi:10.1111/j.1440-1746.1989.tb00836.x

Cited by 9 publications

(3 citation statements)

References 56 publications

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“…[1][2][3] The cell density in the device is crucially important for its efficiency in clinical application. Cell viability and density vary greatly with respect to the biomaterials, and its geometric form is used for anchorage of hepatocytes.…”

Section: Introductionmentioning

confidence: 99%

Preparation and culture of hepatocyte on gelatin microcarriers

2003

J Biomedical Materials Res

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Porous gelatin microcarriers having a diameter of 80-100 microm were prepared by the suspension method using toluene as the oil phase. Rat hepatocytes were cultured on gelatin and cytodexIII microcarriers. The cells retained its spherical shape, which is similar in vivo, and showed no morphological changes to the flat state. Hepatocyte aggregates on microcarriers maintained higher metabolic functions than monolayer cells. Pore size of microcarrier plays an important role in the attachment and metabolic function of cells in culture. Phase-contrast micrograph and cell activity showed that hepatocytes cultures on gelatin microcarrier of <1 microm pore size is better than that of 5-10 microm.

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Section: Introductionmentioning

confidence: 99%

Preparation and culture of hepatocyte on gelatin microcarriers

2003

J Biomedical Materials Res

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“…Despite numerous techniques for blood purification including perfusion over beds of sorbents, hemofiltration, and dialysis ( 1), there is still no adequate form of treatment for acute hepatic failure, and the mortality rate remains above 80% ( 2). The complexity of the liver metabolic functions to be replaced limits the usefulness of any one nonbiological support system, and interest has thus increased in the development of a hybrid artificial liver (HAL) in which cultured cells would supplement the functions of the failing liver.…”

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confidence: 99%

Cytotoxicity of Bile in Human Hep G2 Cells and in Primary Cultures of Rat Hepatocytes

et al. 1998

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There has been increasing interest in the development of a hepatocyte bioreactor for the treatment of acute hepatic failure; however, little is known about the effect of hepatocyte byproducts on the viability of the cells in the bioreactor environment. We investigated the effects of increasing concentrations of bile on the growth and viability of the human hepatoma cell line Hep G2 and on the cytochrome P-450 content and dependent mixed function oxidase (MFO) activities, reduced glutathione (GSH) content, and glutathione S-transferase (GST) activity of primary cultures of rat hepatocytes. Our purpose was to determine whether or not it would be necessary to pretreat the plasma from patients with acute liver failure to remove elevated bile concentrations which might be toxic to the hepatocytes in an artificial liver device. Bile was found to inhibit Hep G2 cell growth at concentrations as low as 0.1% and to decrease viability at concentrations above 0.5%. The cytochrome P-450 and GSH contents and the activities of the MFO system and of GST were decreased in the primary cultures of hepatocytes following 24 h treatment with concentrations of bile at and above 0.5%. The MFO activities associated with different cytochrome P-450 isoenzymes decreased to different extents in the presence of bile with the O-dealkylation of pentoxyresorufin being more labile than that of ethoxyresorufin. Our data indicate that elevated bile concentrations are cytotoxic to liver cells, and it may be necessary to pretreat patient plasma to decrease its bile content to protect the cells during the clinical operation of a hepatocyte bioreactor device.

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“…Despite the great advances in blood purification technologies of the present age, there still remain a vast number of patients suffering from severe liver insufficiency such as fulminant hepatitis who cannot be saved by treatments utilizing existing blood purification techniques such as dialysis, hemoperfusion, and plasmapheresis. It has been pointed out that the survival rates of the patients with fulminant hepatitis undergoing these treatments are quite unsatisfactory, being as low as 20 to 30% (1). Therefore, a bioartificial liver, or a hybrid-type artificial liver, that utilizes heterologous living hepatocytes cultured in a mass transfer device is needed to assist the metabolic functions of the deteriorated liver.…”

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confidence: 99%

Packed‐Bed Type Reactor to Attain High Density Culture of Hepatocytes for Use as a Bioartificial Liver

1997

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In an attempt to develop a bioartificial liver using cultured hepatocytes, we investigated the short-term and long-term viability and metabolic functions of hepatocytes cultured in a new type of packed-bed type reactor using reticulated polyvinyl formal (PVF) resin as a supporting material. Perfusion culture experiments using this reactor, as well as monolayer cultures using conventional collagen-coated Petri dishes as control experiments, were performed. It was found that the highest density of immobilized hepatocytes attained with PVF resin was on the order of lo7 cells/cm3 PVF and that hepatocytes cultured in this type of module for up to a week showed a sufficient level of liver-specific metabolic functions, such as ammonium metabolism, urea-N synthesis, and albumin secretion, to be comparable to those in the monolayer culture. It is concluded that the packed-bed reactor system utilizing PVF resin is a promising means to develop a bioartificial organ using hepatocytes.

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Strategies for hepatic support in acute liver failure: Role of extracorporeal devices versus liver transplantation

Cited by 9 publications

References 56 publications

Preparation and culture of hepatocyte on gelatin microcarriers

Preparation and culture of hepatocyte on gelatin microcarriers

Cytotoxicity of Bile in Human Hep G2 Cells and in Primary Cultures of Rat Hepatocytes

Packed‐Bed Type Reactor to Attain High Density Culture of Hepatocytes for Use as a Bioartificial Liver

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