Strategies for developing decellularized corneal scaffolds

Lynch, Amy; Ahearne, Mark

doi:10.1016/j.exer.2012.12.012

Cited by 113 publications

(97 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Decellularized scaffolds require that cellular and nuclear components of the tissue be removed while the composition and structure of the ECM are maintained (18,20). This approach to biologic scaffold generation has been successful with a variety of tissue types and the resultant acellular ECM has been examined in both pre-clinical studies and human clinical applications (22,(40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dextran Preserves Native Corneal Structure During Decellularization

Lynch

Wilson

Ahearne

2016

Tissue Engineering Part C: Methods

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…These scaffolds often fail to replicate the complex native structural and biochemical architecture of cornea (18).…”

Section: Introductionmentioning

confidence: 99%

Dextran Preserves Native Corneal Structure During Decellularization

Lynch

Wilson

Ahearne

2016

Tissue Engineering Part C: Methods

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the future, decellularized whole corneas could represent a new alternative to corneal human donors in corneal transplants, 14 and these organs could have clinical usefulness for the treatment of patients with SCL damage. Furthermore, once we have demonstrated that whole-porcine corneas can be decellularized, future studies should determine the validness of these methods for decellularizing corneas obtained from other animals, or even from human origin.…”

Section: Discussionmentioning

confidence: 99%

“…11,14 In addition, most published protocols used only the central corneal button, and no experience is available on decellularization of the whole cornea, including the SCL. Decellularization of animal corneas including the SCL could be useful for the generation of complete bioengineered corneas including the area where cornea stem cells reside, and it opens the door to the possibility of generating whole-cornea substitutes for clinical use.…”

Section: Discussionmentioning

confidence: 99%

Effects of Detergent-Based Protocols on Decellularization of Corneas With Sclerocorneal Limbus. Evaluation of Regional Differences

González‐Andrades

Carriel

Rivera-Izquierdo

et al. 2015

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

Citation: González-Andrades M, Carriel V, Rivera-Izquierdo M, et al. Effects of detergent-based protocols on decelluarization of corneas with sclerocorneal limbus. Evaluation of regional differences. Tran Vis Sci Tech. 2014;4(2):13, http://tvstjournal. org/doi/full/10.1167/tvst. 4.2.13, doi: 10.1167/tvst.4.2.13 Purpose: In this work, we decellularized whole porcine corneas including the sclerocorneal limbus (SCL) and we evaluated regional differences in order to identify an efficient method to decellularize whole corneas for future clinical use. Methods:We analyzed the efficiency of four decellularization protocols based on benzalkonium chloride (BAK), Igepal, sodium dodecyl sulfate (SDS), and Triton X-100 detergents on whole porcine corneas.Results: Results showed that the decellularization efficiency of most protocols was low, with the specific protocol resulting in more efficient levels of decellularization being 0.1% SDS for 48 hours, especially in the medium and posterior cornea regions. A significant correlation was found between the decellularization efficiency and the concentration of agent used (P ¼ 0.0174; r ¼ 0.1540), but not for the incubation time (P . 0.05). The analysis of cornea components preservation demonstrated that all protocols were able to preserve the integrity of the Bowman's layer and Descemet's membrane. Although the collagen structure was partially altered, the global decellularization groups showing highest preservation of the ECM collagen contents and orientation were Igepal and SDS, with differences among the three regions of the cornea. All global groups showed high levels of proteoglycan and glycoprotein preservation after decellularization, with the best results were found in the SDS group followed by the Igepal group.Conclusions: These results suggest that very powerful protocols are necessary for whole-cornea decellularization. For the generation of lamelar corneas for clinical use, decellularization regional differences should be taken into account.Translational Relevance: Decellularized whole corneas may be potential therapeutic agents for lamelar keratoplasty.

show abstract

“…Transplanting a cell substitute along with the structural support to undertake the critical functions in corneal homeostasis performed by keratocytes is essential because they produce factors such as collagen, proteoglycans, and metalloproteinases, which are indispensable for the health of the cornea and the long-term maintenance of corneal transparency [24]. To repopulate the scaffolds, h-ADASCs were used, which have been shown to be a perfect source of autologous stem cells for the development of tissue-engineered corneas and avoid the limitations of corneal cells [6,8,25].…”

Section: Discussionmentioning

confidence: 99%