Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model

Abstract

survival was demonstrated in all the membranes after three months follow-up. A slight reduction in the extrusion rate of h-ADASC colonized materials was observed. No significant differences between the groups with and without h-ADASC were detected respect to transparency or neovascularization. We propose PEA with low hydroxylation as a scaffold for the anchoring ring of future keratoprosthesis.

Osteogenic differentiation of mesenchymal stem cells using hybrid nanofibers with different configurations and dimensionality

“…The internal hole on the gel O‐rings was created to increase the diffusion of nutrients and to provide “place” on the supporting gel where only nanofibers exist. The biocompatibility of hydrogel was previously proved in both in vitro and in vivo studies …”

“…The biocompatibility of hydrogel was previously proved in both in vitro [20][21][22] and in vivo studies. [23][24][25] Briefly, a mixture of the monomers EA (99% purity, Sigma-Aldrich) and HEA (96% purity, Sigma-Aldrich), in 70/ 30 mass %, containing 1% of ethylenglycol dimethacrylate (98% purity, Sigma Aldrich) as cross-linker and 1% benzoine (98% purity (Sharlau) as initiator was polymerized between two glass plates at room temperature under UV radiation for 24 h. The resulting polymer sheets were purified by boiling in excess ethanol (four changes of ethanol, 8 h each boiling) and dried in vacuum to constant weight. Each O-ring was further coated with electrospun nanofibers, random or aligned, to overlay the sample, including the 6 mm environmental hole punched in the middle (providing better nutrition and preserving a space where nanofibers only exist).…”

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

“…For instance, Gómez Ribelles et al (33) used copolymers based on hydrophobous EA and containing a small fraction of a hydrophylic component for induction joint cartilage regeneration. In addition, poly(EA) copolymers were implanted inside rabbit cornea as a model for a keratoprosthesis (34), whereas scaffolds with aligned channels based on acrylate copolymers were studied as colonizable structures both "in vitro" with neural progenitor cells and "in vivo" (35).…”

Mallas macroporosas bioestables con aplicación en implantes protésicos y trasplante de células.

“…En algunas de estas aplicaciones, se requieren soportes porosos en forma de membranas macroporosas flexibles y muy delgadas. Este es el caso del anillo de anclaje de una prótesis de córnea, que ha de ser un material fino y bioestable 42,[53][54][55][56][57][58][59][60][61][62][63] , donde la lente sintética queda rodeada por un material macroporoso (anillo de anclaje), que es invadido por el tejido huésped, y de esta manera la prótesis queda fija de manera permanente; o cuando las células han de ser aisladas del sistema inmunológico del organismo para evitar el rechazo, como en la implantación de células pancreáticas  responsables de la producción de insulina, o aplicaciones donde el tejido regenerado debe soportar considerables esfuerzos mecánicos, como es el caso de prótesis de discos intervertebrales o en cementos óseos. En otros casos, se requiere un parche bioestable también portador de células, sistemas que mantengan las células alojadas, como vendas y apósitos, que pueda presionar sobre el tejido para inducir un efecto paracrino a través de la entrega continua de factores de crecimiento, pudiendo encontrar aplicación en la curación de heridas, y la regeneración de la piel o de la córnea [64][65][66][67][68][69][70][71][72][73] .…”