Strand displacement amplification for ultrasensitive detection of human pluripotent stem cells

Wu, Wei; Mao, Yiping; Zhao, Shufang; Lu, Xuewen; Liang, Xingguo; Zeng, Lingwen

doi:10.1016/j.aca.2015.04.003

Cited by 12 publications

(6 citation statements)

References 23 publications

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“…However, probe-based lateral flow assay in combination with SDA could provide robust multiplexing detection higher than their peer antibodies [84]. For example, our group [15,24,36] and other researchers [3,100,101] demonstrated that SDA can be used to amplify short target recognition sequences, for example, aptamers, after target binding and then integrated with lateral flow biosensors for analysis. Therefore, preheat denaturation of templates, background signals, and unspecificity encountered during the use of long nucleic acid templates or the presence of DNA contaminants are prevented [102,103].…”

Section: Discussion Conclusion and Future Perspectivesmentioning

confidence: 99%

“…To overcome contamination and detect live targets accurately, gDNA extraction free isothermal amplification methods have been adopted, which use extracellular compartment recognizing molecules such as aptamers. We reported ultrasensitive aptamer-based biosensors for the detection of live pathogens including E. coli and S. enteritidis [15,24,36]. In these methods, a dual aptamer system recognizing two extracellular membrane components are used.…”

Section: Sample Preparationmentioning

confidence: 99%

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Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Zeng¹,

Mukama²,

Lu³

et al. 2019

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

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The identification of various targets such as bacteria, viruses, and other cells remains a prerequisite for point-of-care diagnostics and biotechnological applications. Nucleic acids, as encoding information for all forms of life, are excellent biomarkers for detecting pathogens, hereditary diseases, and cancers. To date, many techniques have been developed to detect nucleic acids. However, most of them are based on polymerase chain reaction (PCR) technology. These methods are sensitive and robust, but they require expensive instruments and trained personnel. DNA strand displacement amplification is carried out under isothermal conditions and therefore does not need expensive instruments. It is simple, fast, sensitive, specific, and inexpensive. In this chapter, we introduce the principles, methods, and updated applications of DNA strand displacement technology in the detection of infectious diseases. We also discuss how robust, sensitive, and specific nucleic acid detection could be obtained when combined with the novel CRISPR/Cas system.

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Section: Discussion Conclusion and Future Perspectivesmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Zeng¹,

Mukama²,

Lu³

et al. 2019

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

Self Cite

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“…The response of the optimized LFB was highly linear over a range of 1 × 10 4 to 2 × 10 5 human stem cells. Later on, the same group has developed a nucleic acid lateral flow to enhance hPSCs detection efficacy [155]. Firstly, magnetic beads coated with antibody were used to enrich the target cells and the secondary antibody linked with an oligonucleotide was used as amplification method.…”

Section: Aunps-based Stem Cell Labeling and Trackingmentioning

confidence: 99%

“…Nowadays, dark-field microscopy [159], magnetic resonance imaging [160,161], ultrasound imaging [162], two-photon luminescence imaging [163], photoacoustic tomography [164], optical coherence tomography (OCT) [165] and X-ray computed tomography [166] have been investigated individually or combined in nanomedicine, enabling cells tracking. Due to their scattering and light absorption properties, as well as their high photostability compared to traditional dyes or semiconductor crystals, AuNPs have been used as contrast agent in order to provide a better visualization and thus improve the performance of such techniques [157], For example, 30 nM of AuNRs injected to the anterior chamber of mice eyes have produced a contrast [155]. Copyright 2015, with permission from Elsevier signal 50% higher than the control with saline injection, making them suitable to be used with OCT (Fig.…”

Section: Aunps-based Cellular Imagingmentioning

confidence: 99%

Recent advances using gold nanoparticles as a promising multimodal tool for tissue engineering and regenerative medicine

Vial¹,

Reis²,

Oliveira³

2017

Current Opinion in Solid State and Materials Science

143

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Gold nanoparticles (AuNPs) have arisen a lot of interest in the clinical realms of nanomedicine. Despite the large advances made in cancer research using AuNPs, their use in tissue engineering and regenerative medicine (TERM) is still in its infancy. Herein, it is discussed the properties, functionalization, and emerging use of AuNPs as a multifunctional and multimodal platform for drug delivery, phototherapy, diagnostic and cell imaging purposes. Moreover, the recent reports related to the ability of AuNPs to enhance stem cell differentiation for bone tissue engineering, to enhance the mechanical and adhesive properties of scaffolds and surface topography to guide cell behaviors are addressed.

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“…For example, a multiplex iNAAT was developed by combination of NASBA with microarray analysis . For the detection of single pathogenic bacteria or viruses, lateral flow dipsticks were combined, e.g., with LAMP, SDA, , and RPA. , A first miniaturized RPA on a lab-on-disk (LoD) platform was performed by Lutz et al Later, Kim et al integrated chambers for magnetic bead-based DNA extraction, RPA, and amplicon quantification on lateral flow strips into one LoD instrumentation. Thus, Salmonella in milk was detected within 30 min.…”

mentioning

confidence: 99%

On-Chip Isothermal Nucleic Acid Amplification on Flow-Based Chemiluminescence Microarray Analysis Platform for the Detection of Viruses and Bacteria

et al. 2015

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This work presents an on-chip isothermal nucleic acid amplification test (iNAAT) for the multiplex amplification and detection of viral and bacterial DNA by a flow-based chemiluminescence microarray. In a principle study, on-chip recombinase polymerase amplification (RPA) on defined spots of a DNA microarray was used to spatially separate the amplification reaction of DNA from two viruses (Human adenovirus 41, Phi X 174) and the bacterium Enterococcus faecalis, which are relevant for water hygiene. By establishing the developed assay on the microarray analysis platform MCR 3, the automation of isothermal multiplex-amplification (39 °C, 40 min) and subsequent detection by chemiluminescence imaging was realized. Within 48 min, the microbes could be identified by the spot position on the microarray while the generated chemiluminescence signal correlated with the amount of applied microbe DNA. The limit of detection (LOD) determined for HAdV 41, Phi X 174, and E. faecalis was 35 GU/μL, 1 GU/μL, and 5 × 10(3) GU/μL (genomic units), which is comparable to the sensitivity reported for qPCR analysis, respectively. Moreover the simultaneous amplification and detection of DNA from all three microbes was possible. The presented assay shows that complex enzymatic reactions like an isothermal amplification can be performed in an easy-to-use experimental setup. Furthermore, iNAATs can be potent candidates for multipathogen detection in clinical, food, or environmental samples in routine or field monitoring approaches.

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Strand displacement amplification for ultrasensitive detection of human pluripotent stem cells

Cited by 12 publications

References 23 publications

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Recent advances using gold nanoparticles as a promising multimodal tool for tissue engineering and regenerative medicine

On-Chip Isothermal Nucleic Acid Amplification on Flow-Based Chemiluminescence Microarray Analysis Platform for the Detection of Viruses and Bacteria

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