Strain‐Specific Impact of <i>Fusobacterium nucleatum</i> on Neutrophil Function

Kurgan, Şivge; Kansal, Shevali; Nguyen, Daniel; Stephens, Danielle; Koroneos, Yannis; Hastürk, Hatice; Dyke, Thomas E. Van; Kantarcı, Alpdoğan

doi:10.1902/jop.2016.160212

Cited by 41 publications

(49 citation statements)

References 53 publications

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“…Kugan et al . examined various strains, including ATCC 25586 and ATCC 10953, with regard to apoptosis, phagocytosis, superoxide generation and proinflammatory cytokine release by neutrophils, and found an F. nucleatum strain‐specific impact on neutrophil function [42]. Conversely, Bhattacharyya et al .…”

Section: Discussionmentioning

confidence: 99%

Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

Nishi

Hosomi

Ohta

et al. 2020

Clinical and Experimental Immunology

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Stroke can be a cause of death, while in non-fatal cases it is a common cause of various disabilities resulting from associated brain damage. However, whether a specific periodontal pathogen is associated with increased risk of unfavorable outcome after stroke remains unknown. We examined risk factors for unfavorable outcome following stroke occurrence, including serum antibody titers to periodontal pathogens. The enrolled cohort included 534 patients who had experienced an acute stroke, who were divided into favorable (n = 337) and unfavorable (n = 197) outcome groups according to modified ranking scale (mRS) score determined at 3 months after onset (favorable = score 0 or 1; unfavorable = score 2-6). The associations of risk factors with unfavorable outcome, including serum titers of IgG antibodies to 16 periodontal pathogens, were examined. Logistic regression analysis showed that the initial National Institutes of Health stroke scale score [odds ratio (OR) = 1·24, 95% confidence interval (CI) = 1·18-1·31, P < 0·001] and C-reactive protein (OR = 1·29, 95% CI = 1·10-1·51, P = 0·002) were independently associated with unfavorable outcome after stroke. Following adjustment with those, detection of the antibody for Fusobacterium nucleatum ATCC 10953 in serum remained an independent predictor of unfavorable outcome (OR = 3·12, 95% CI = 1·55-6·29, P = 0·002). Determination of the antibody titer to F. nucleatum ATCC 10953 in serum may be useful as a predictor of unfavorable outcome after stroke.

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Section: Discussionmentioning

confidence: 99%

Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

Nishi

Hosomi

Ohta

et al. 2020

Clinical and Experimental Immunology

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“…A. actinomycetemcomitans and F. nucleatum are known as bacteria that are involved with periodontal diseases [21,46 ]. The role of bacterial communication in the disease process outlines F. nucleatum as a key coadjuvant [ 22 ] that is able to co-aggregate with A. actinomycetemcomitans and intensifies damages around tooth-supporting tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Another important Gram-negative bacterium involved with periodontal disease pathogenesis is Fusobacterium nucleatum [19,20]. This bacterial species is known to coaggregate with early and late colonizers by its proteins and receptors available in the out membrane, therefore contributing to periodontal disease progression [ 21 ]. In this context, the adhesins RadD and Fap2 were identified as two important virulence factors [ 22 ] that have binding properties for Gram-positive species and Porphyromonas gingivalis, respectively.…”

Section: Introductionmentioning

confidence: 99%

Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

et al. 2018

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Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are strongly associated with periodontitis, and their evaluations are relevant to understand their role in the etiology and progression of periodontal diseases. In this study, the qualitative and quantitative detection of A. actinomycetemcomitans and F. nucleatum, as well as their genetic diversity, were evaluated in individuals with gingivitis, chronic periodontitis and periodontally healthy. In addition, the biotyping, serotyping, and prevalence of the ltx and cdt genes in A. actinomycetemcomitans were also determined. Subgingival biofilms obtained from gingivitis (70), periodontitis (75) and healthy (95) individuals were analyzed by cultures and PCR. Bacterial typing and presence of ltx and cdt genes in A. actinomycetemcomitans were also verified. DNA from A. actinomycetemcomitans and F. nucleatum was detected respectively, in 65.7% and 57.1% of gingivitis, 80% and 68% of periodontitis, and 57.8% and 37.8% of healthy. A. actinomycetemcomitans from gingivitis were biotypes I, II, IV, V, and X, and serotypes a, c, and e. In periodontitis, biotypes II, VI, and X, and serotypes a, b, and c were found. In healthy subjects, biotypes II and X, and serotypes b and c were found. The LTX and ltxA were observed in strains from gingivitis and periodontitis pockets. Subsequently, our data also showed no direct relationship between ltxA gene expression and leukotoxin gene 530-bp presence. On the other hand, cdt gene predominated during the inflammatory disease process. Our results strongly support a role of A. actinomycetemcomitans and F. nucleatum in advanced stage of periodontal disease.

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“…Neutrophils are an effective defense against invading microorganisms [17]. Phagocytosis is a key strategy for neutrophils in killing pathogens [18]. e phagocytic rate and phagocytic index were examined to investigate the activation of neutrophils [19].…”

Section: Effects Of Lvfp On Neutrophil Phagocytosis In Cy-inducedmentioning

confidence: 99%

Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum vicaryi L. Fruit

Liu

Wang

Ren

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Ligustrum vicaryi L. is a hybrid of Ligustrum ovalifolium Hassk. var. aureo-marginatum and Ligustrum vulgale L., belonging to the Oleaceae family. It is often used as an ornamental shrub due to its golden leaves. However, its medical value is yet to be discovered. Recently, plant polysaccharides have attracted comprehensive attention owing to their biological properties, including immunomodulatory and antioxidant activities. This study aimed to extract, purify, and characterize the polysaccharide from the Ligustrum vicaryi L. fruit and investigate its immunomodulatory and antioxidant activities. The Ligustrum vicaryi L. fruit polysaccharide (LVFP) was obtained by ultrasonic extraction, ethanol precipitation, macroporous resin separation, and dialysis bag purification. The physicochemical properties of the LVFP were elucidated using Fourier-transform infrared spectrometry, high-performance ion chromatography, and high-performance gel filtration chromatography. The results indicated that the LVFP consisted of rhamnose, arabinose, galactose, and glucose in a ratio of 1.79 : 7.55 : 4.58 : 1.54, and its molecular weight was 88,949 Da. The immunomodulatory and antioxidant activities of the LVFP were investigated using a cyclophosphamide- (Cy-) induced immunosuppressed mouse model. The results demonstrated that the LVFP significantly increased spleen and thymus indexes, enhanced the phagocytic function of neutrophils, activated B and T lymphocytes, and upregulated serum levels of IL-10 and TNF-α. Moreover, we observed that the LVFP relieved Cy-induced liver damage by increasing superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) levels. These results suggested that the LVFP has the immunomodulatory and antioxidant activities, therefore laying a foundation for the application of the LVFP in the pharmaceutical and functional food industries.

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Strain‐Specific Impact of Fusobacterium nucleatum on Neutrophil Function

Cited by 41 publications

References 53 publications

Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum vicaryi L. Fruit

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