Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions

Abstract

Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are strongly associated with periodontitis, and their evaluations are relevant to understand their role in the etiology and progression of periodontal diseases. In this study, the qualitative and quantitative detection of A. actinomycetemcomitans and F. nucleatum, as well as their genetic diversity, were evaluated in individuals with gingivitis, chronic periodontitis and periodontally healthy. In addition, the biotyping, serotyping, and prevalence of the ltx and cdt genes in A. actinomycetemcomitans were also determined. Subgingival biofilms obtained from gingivitis (70), periodontitis (75) and healthy (95) individuals were analyzed by cultures and PCR. Bacterial typing and presence of ltx and cdt genes in A. actinomycetemcomitans were also verified. DNA from A. actinomycetemcomitans and F. nucleatum was detected respectively, in 65.7% and 57.1% of gingivitis, 80% and 68% of periodontitis, and 57.8% and 37.8% of healthy. A. actinomycetemcomitans from gingivitis were biotypes I, II, IV, V, and X, and serotypes a, c, and e. In periodontitis, biotypes II, VI, and X, and serotypes a, b, and c were found. In healthy subjects, biotypes II and X, and serotypes b and c were found. The LTX and ltxA were observed in strains from gingivitis and periodontitis pockets. Subsequently, our data also showed no direct relationship between ltxA gene expression and leukotoxin gene 530-bp presence. On the other hand, cdt gene predominated during the inflammatory disease process. Our results strongly support a role of A. actinomycetemcomitans and F. nucleatum in advanced stage of periodontal disease.

Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults

“…F. nucleatum is an important microorganism in the development of dental biofilms; it can adhere to most plaque bacteria and is involved in linking early and late migratory microorganisms [27]. In a previous study, Arenas Rodrigues et al [28] isolated F. nucleatum from individuals with both healthy periodontal status and periodontal disease, and Zhou et al [29] found that 58.5% of healthy young adults were positive for this bacterium.…”

Clinical and radiographic peri‐implant outcomes of short dental implants placed in posterior jaws of patients with treated generalized aggressive periodontitis: A 3‐year follow‐up study

“…11 Moreover, periodontal sites harboring Aggregatibacter actinomycetemcomitans demonstrate deeper probing depths (PDs) with or without clinical signs of inflammation. 12 Research suggests that a significant correlation exists in the overall periodontal conditions with the soft tissue conditions around dental implants that indicates a possible spread of infection from periodontally compromised/untreated sites to the peri-implant sulci. 13,14 To date, no comparison of clinical, radiographic, and implant survival rates of SDIs has been provided in a group of treated GAgP patients and periodontally healthy controls.…”

Insights into the study and origin of the citrullinome in rheumatoid arthritis

“…Aa is a periodontal pathogen associated with severe forms of periodontitis, [117][118][119][120][121] both localized aggressive periodontitis (LAP) and chronic periodontitis. 117,118,[122][123][124][125][126][127][128][129][130][131] In contrast to other oral bacteria, Aa has a unique capacity to induce neutrophil hypercitrullination, suggesting a potential role of this bacterium in generating the periodontal citrullinome. 72 Similar to host-immune pore-forming pathways, Aa hyperactivates PADs by inducing membranolytic damage on neutrophils.…”