Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults

Choi, Hoo‐Kyun; Kim, Eunhye; Kang, JiHoon; Kim, Hyun‐Joo; Lee, Ju Youn; Choi, Jaeho; Joo, Ji-Young

doi:10.5051/jpis.2018.48.4.261

Cited by 26 publications

(32 citation statements)

References 41 publications

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“…Many authors consider that the initiation and progression of periodontal diseases are directly linked to the colonisation of microorganisms such as A. actinomycetemcomitans, as well as microorganisms from the red complex, as classified by Socransky [3,4]. Kulekci identified microorganism associations that are destructive to the periodontium in early childhood and in young adults [20]. The present study provided evidence in support of these observations in pre-teen age children and early teens, which is a less studied age group [22][23][24][25][26].…”

Section: Microbial Associationssupporting

confidence: 80%

“…It has been isolated in the largest number of subjects, and in the highest levels [13,14,19]. F. nucleatum is an important microorganism in the formation of dental biofilms by adhering to most plaque bacteria and contributing to the linking of early and late migratory microorganisms [20]. This determines it as an intermediate coloniser and an intermediary between the beneficial microflora and the periodontally pathogenic strains [19].…”

Section: Subgingival Microorganism Counts/abundance Of Subgingival MImentioning

confidence: 99%

“…This determines it as an intermediate coloniser and an intermediary between the beneficial microflora and the periodontally pathogenic strains [19]. F. nucleatum can be isolated from individuals both with or without signs of periodontal disease [13,20]. However, Zhou et al [15] found that only 58.5% of healthy young adults were positive for this bacterium.…”

Section: Subgingival Microorganism Counts/abundance Of Subgingival MImentioning

confidence: 99%

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Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis

Mitova

Rashkova

Popova

2019

Biotechnology & Biotechnological Equipment

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Periodontal diseases, as an important part of oral pathology, have their distinctive characteristics when affecting children and young adults. The aim of this study was to identify the subgingival microorganisms through the use of real-time polymerase chain reaction (PCR) in children at the age of puberty who have plaque-induced gingivitis. The subjects of observation were 60 children, aged between 10 and 14, who did not have any systemic diseases: 30 without gingivitis (up to 25% PBI); 30 with clinically diagnosed plaque-induced gingivitis (over 25% PBI). The clinical status of each child was registered using a custom-made medical card. Gingival sulcus samples were taken with a paper pin from six teeth for real time PCR identification based on nine control strains (a comprehensive sample). Samples were transported in standardised containers. The results showed that the average quantities of the tested microorganisms were between 1 Â 10 2 and 1 Â 10 5 microorganisms per sample. There was a trend towards a putative increase in the average quantities of microorganisms, except for Fusobacterium nucleatum, in children with gingivitis as compared to healthy children. There were associations with up to four microorganisms in healthy children. In children with gingivitis, there was greater diversity of microorganisms, with half of the children from this group having associations with 5-7 microorganisms. The subgingival microflora became more complex in children with gingivitis: the predominant group were microorganisms from the red complex (Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia) and the frequency of microorganisms from the orange complex also increased.

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Section: Microbial Associationssupporting

confidence: 80%

Section: Subgingival Microorganism Counts/abundance Of Subgingival MImentioning

confidence: 99%

Section: Subgingival Microorganism Counts/abundance Of Subgingival MImentioning

confidence: 99%

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Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis

Mitova

Rashkova

Popova

2019

Biotechnology & Biotechnological Equipment

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“…In other pathogenic bacteria, such as the uropathogenic E. coli strain 536 and P. aeruginosa, independent evidence has revealed the presence of secretory bacterial sRNAs within OMVs (Blenkiron et al, 2016;Koeppen et al, 2016). More recently, also periodontal pathogens, such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola, have been reported to secrete sRNAs in OMVs (Choi et al, 2018). Similarly, OMVs secreted by S. Typhimurium were characterized, revealing the presence of RNAs.…”

Section: Characterization Of the Rna Content Of Bacterial Omvsmentioning

confidence: 99%

“…High-throughput sequencing Choi et al, 2018 Salmonella enterica serovar Typhimurium (S. Typhimurium)…”

Section: Rna-seq (Myseq Sequencing) Blenkiron Et Al 2016mentioning

confidence: 99%

Small RNAs in Outer Membrane Vesicles and Their Function in Host-Microbe Interactions

et al. 2020

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Outer membrane vesicles (OMVs) produced by Gram-negative bacteria and their RNA content have recently received increasing attention as novel mediators of host-guest intercellular and interspecies communication. These kinds of vesicles allow not only the export of proteins, but also of DNA and other small RNAs. We previously reported that the production of bacterial vesicles could represent a way to communicate with the surrounding environment. This general mechanism, exploited by bacteria and viruses, allows conveying "messages" to the surrounding cells and tissues, thus leading to the manipulation of the host immune response. Many functions attributed to OMVs are a consequence of their high capability to survive in different environmental stressors and to enter into cells quite easily. As these properties can be exploited to advantage human health, in this review, we summarized the papers that reported the use of OMVs as modulators of cellular activities through the involvement of the RNAs contained within. We also discussed what has been done with OMVs and what remains to be discovered in order to prompt the investigations toward the complete elucidation of the role and functions of these vesicles.

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Detection of colorectal‐cancer‐associated bacterial taxa in fecal samples using next‐generation sequencing and 19 newly established qPCR assays

Senthakumaran,

Tannæs,

Moen

et al. 2024

Molecular Oncology

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We have previously identified increased levels of distinct bacterial taxa within mucosal biopsies from colorectal cancer (CRC) patients. Following prior research, the aim of this study was to investigate the detection of the same CRC‐associated bacteria in fecal samples and to evaluate the suitability of fecal samples as a non‐invasive material for the detection of CRC‐associated bacteria. Next‐generation sequencing (NGS) of the 16S ribosomal RNA (rRNA) V4 region was performed to evaluate the detection of the CRC‐associated bacteria in the fecal microbiota of cancer patients, patients with adenomatous polyp and healthy controls. Furthermore, 19 novel species‐specific quantitative PCR (qPCR) assays were established to detect the CRC‐associated bacteria. Approximately, 75% of the bacterial taxa identified in biopsies were reflected in fecal samples. NGS failed to detect low‐abundance CRC‐associated taxa in fecal samples, whereas qPCR exhibited high sensitivity and specificity in identifying all targeted taxa. Comparison of fecal microbial composition between the different patient groups showed enrichment of Fusobacterium nucleatum, Parvimonas micra, and Gemella morbillorum in cancer patients. Our findings suggest that low‐abundance mucosa‐associated bacteria can be detected in fecal samples using sensitive qPCR assays.

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Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults

Cited by 26 publications

References 41 publications

Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis

Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis

Small RNAs in Outer Membrane Vesicles and Their Function in Host-Microbe Interactions

Detection of colorectal‐cancer‐associated bacterial taxa in fecal samples using next‐generation sequencing and 19 newly established qPCR assays

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