Strain screening and sodium lactate effect on spiramycin production in Streptomyces spiramyceticus

Jun, Zeng; Ye, Rui-fang; Zheng, Yitao; Mao, Quangui; Lv, Heping; Shi, Tingting

doi:10.1007/s11164-015-2107-1

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Acyl-CoA synthetase (AS) activity, acylkinase (AK) activity, and acyl phosphotransferase (APT) activity were assayed as described by Zeng et al (2016). One unit (U) of enzymatic activity was defined as the amount of enzyme that catalyzed the formation of 1 µmol of each enzymatic reaction product per hour under the abovementioned conditions.…”

Section: Enzyme Assaymentioning

confidence: 99%

Effects of precursors on kitasamycin production in Streptomyces kitasatoensis

Xiong¹,

Ye²,

Hu³

et al. 2019

Biosci. J.

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To improve kitasamycin biosynthesis by Streptomyces kitasatoensis Z-7, the addition of two precursors, sodium acetate and ethyl acetate, to the fermentation medium was evaluated. Ethyl acetate was the most effective precursor compared with control conditions; In a 15-L fermentor, the kitasamycin titer was 21% higher when 0.48% ethyl acetate was added compared to control conditions. Content of the A 5 component increased by 5.1%, and the A 4 content decreased slightly compared to that of the control. During kitasamycin synthesis, intracellular and extracellular concentrations of acetic acid were higher for S. kitasatoensis Z-7 supplemented with ethyl acetate than for the non-supplemented strain, and the activities of acyl-CoA synthetases, acyl-phosphotransferases, and acyl-kinases were also significantly increased, suggesting that increased acetyl-CoA levels can explain the high kitasamycin titer. These findings may improve the industrialscale production of kitasamycin for clinical use, and the addition of 0.48% ethyl acetate as precursors in the medium at the beginning of cultivation was a new method to mitigate the negative influence on the cell growth of excess precursor.

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Section: Enzyme Assaymentioning

confidence: 99%

Effects of precursors on kitasamycin production in Streptomyces kitasatoensis

Xiong¹,

Ye²,

Hu³

et al. 2019

Biosci. J.

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Synthesis of core-shell molecularly imprinted polymers (MIP) for spiramycin I and their application in MIP chromatography

Liu

Wan

Cao

2018

Process Biochemistry

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Influence of Al³⁺ on the titer of spiramycin and effective components in fermentor

et al. 2017

Preparative Biochemistry & Biotechnology

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Spiramycin is a multicomponent antibiotic, and different components have different antibacterial activities. In Streptomyces spiramyceticus 16-10-2, spiramycin II and spiramycin III (SPMII and SPMIII) are the main components, while spiramycin I (SPMI) needs to be controlled below 12%. Based on this, the influences of Al on total spiramycin titer and components were investigated in this work. Those experiments were mainly performed in 15 L fermentor and Al made a great improvement in spiramycin titer. The optimal adding concentration and adding time of Al were 0.32 g/L at 12 hr. Under this condition, spiramycin titer was increased by 19.51% compared with the control. Moreover, the percentage of SPMII and SPMIII was increased by 7.14%. At the same time, the time of mycelia autolysis was lengthened. In addition, the specific activities of acetyl-CoA synthetase, acetate kinase, acetylphosphotransferase, and acylating enzyme were much higher than those of control. The content of acetic acid and succinic acid was beyond 3 and 4.5 times than that of control, respectively.

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Strain screening and sodium lactate effect on spiramycin production in Streptomyces spiramyceticus

Cited by 3 publications

References 20 publications

Effects of precursors on kitasamycin production in Streptomyces kitasatoensis

Effects of precursors on kitasamycin production in Streptomyces kitasatoensis

Synthesis of core-shell molecularly imprinted polymers (MIP) for spiramycin I and their application in MIP chromatography

Influence of Al³⁺ on the titer of spiramycin and effective components in fermentor

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Strain screening and sodium lactate effect on spiramycin production in Streptomyces spiramyceticus

Cited by 3 publications

References 20 publications

Effects of precursors on kitasamycin production in Streptomyces kitasatoensis

Effects of precursors on kitasamycin production in Streptomyces kitasatoensis

Synthesis of core-shell molecularly imprinted polymers (MIP) for spiramycin I and their application in MIP chromatography

Influence of Al3+ on the titer of spiramycin and effective components in fermentor

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Influence of Al³⁺ on the titer of spiramycin and effective components in fermentor