Strain identification in Rhizobium by starch gel electrophoresis of isoenzymes

Engvild, Kjeld C.; Nielsen, Gunnar Gissel

doi:10.1007/bf02181864

Cited by 11 publications

(8 citation statements)

References 12 publications

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“…Furthermore, with the exceptions of the studies of Pinero et al (31) and Eardly et al (11), the reported electrophoretic studies on Rhizobium species have been based on only a few enzyme loci. However, these studies have shown that numerous allelic variants exist for those loci examined (13,14,18,27,28,45,46). In this study, we examined 44 different enzyme loci, of which 28 loci provided staining of sufficient intensity and resolution for reliable genetic interpretation.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses

Demezas

Reardon

Watson

et al. 1991

Appl Environ Microbiol

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Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium kguminosarum bv. trifolii, 1 R. kguminosarum bv. viciae, and 2 R. meliloti strains. Ailozyme analysis at 28 loci revealed 16 electrophoretic types. The mean genetic distance between electrophoretic types of R. leguminosarum and R. meliloti was 0.83. Within R. keguminosarum, the single strain of bv. viciae differed at an average of 0.65 from strains of bv. trifolii, while electrophoretic types of bv. trifolil differed at a range of 0.23 to 0.62. Analysis of RFLPs around two chromosomal DNA probes also delineated 16 unique RFLP patterns and yielded genetic diversity similar to that revealed by the allozyme data. Analysis of RFLPs around three Sym (symbiotic) plasmid-derived probes demonstrated that the Sym plasmids reflect genetic divergence similar to that of their bacterial hosts. i2hE large genetic distances between many strains precluded reliable estimates of their genetic relationships.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses

Demezas

Reardon

Watson

et al. 1991

Appl Environ Microbiol

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“…The starch gel electrophoresis isoenzyme pattern types were determined on sonicated extracts of cells as described (Engvild and Nielsen, 1985). The gels were stained for esterases, 3-hydroxybutyrate dehydrogenases, arabinose dehydrogenases and sorbitol dehydrogenases.…”

Section: Lsoenzyme Electrophoresismentioning

confidence: 99%

Parallel variation in isoenzyme and nitrogen fixation markers in a Rhizobium population

1990

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Twenty isolates of Rhizobium leguminosarum bv. viceae were isolated at random from one field and examined for symbiotic plasmid fragment length polymorphisms and for isoenzyme patterns. The latter are most probably chromosome markers. With one exception both methods separated the isolates into the same 13 different groups. The largest group was represented 7 times according to isoenzymes and 8 times according to RFLP. This fixed non-random association of plasmid and chromosomal genotypes is consistent with a clonal population structure; it indicates limited exchange of plasmids under natural conditions. Seventeen isolates of 11 groups were highly effective and 2 isolates in one group almost ineffective.

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“…These results were also found by LEMOS (1994) who confirmed the four homology groups already defined. The enzymatic polymorphism tests might be an efficient way to characterize strains and establish the genetic structure of rhizobia populations (ENGVILD & NIELSEN, 1985;ENGVILD et al, 1990). The restricted differences found in this study (only between variants of strain 5019) might be related to the small number of enzymes tested.…”

Section: Resultsmentioning

confidence: 92%

“…Isolates was grown in 50ml of tryptoneyeast medium (TY) (ENGVILD & NIELSEN, 1985) for 72 hours at 28 o C and 120rpm. After centrifugation (15,000 X g, 4 o C -20min), the bacterial pellet was washed several times in saline solution.…”

Section: Methodsmentioning

confidence: 99%

Characterization of variants of Bradyrhizobium elkanii and B. japonicum and symbiotic behaviour in soybeans

et al. 2004

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Strain identification in Rhizobium by starch gel electrophoresis of isoenzymes

Cited by 11 publications

References 12 publications

Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses

Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses

Parallel variation in isoenzyme and nitrogen fixation markers in a Rhizobium population

Characterization of variants of Bradyrhizobium elkanii and B. japonicum and symbiotic behaviour in soybeans

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