Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability

Gączarzewicz, Dariusz; Udała, J.; Piasecka, Małgorzata; B, Błaszczyk; Stankiewicz, Tomasz

doi:10.3906/biy-1412-76

Cited by 30 publications

(21 citation statements)

References 58 publications

(139 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Liquid preservation of boar sperm under hypothermic conditions, 5°C, may be connected with alterations in the plasma membrane stability and disorders of mitochondrial transmembrane potential and oxidoreductive capability affecting the sperm motility (Gączarzewicz, Udała, Piasecka, Błaszczyk, & Stankiewicz, ). However, the supplementation of commercial extenders with ostrich egg yolk lipoproteins seemed to improve the metabolic efficiency of boar spermatozoa during storage for 3 days at 5°C (Dziekońska, Kinder, Fraser, Strzeżek, & Kordan, ).…”

Section: Temperaturementioning

confidence: 99%

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

Contents In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection. In both methods and due to the sensitivity of boar sperm to osmotic and temperature changes, there is a loss in the quality of the initial sample; however, slow freezing in boar semen has greater deleterious effects on the sample that are reflected in the pregnancy rates and number of live births. Therefore, only 1% of all inseminations are done with frozen semen. The aim of this review is to provide advances and results of studies conducted on the preservation of boar semen, delving more deeply into the critical points that each of the preservation techniques presents, including bacterial contamination, extender components, temperature, ice nucleation, use of additives in extenders and the main deleterious effects on sperm quality.

show abstract

Section: Temperaturementioning

confidence: 99%

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The pH of the semen samples was measured using a two-point calibrated (pH 6.0 and 8.0; Poch, Poland) pH-meter (CP-315M, Elmetron; electrode type ERH-13-6, Hydromet, Poland). The measurement of aspartate aminotransferase (AAT; EC 2.6.1.1) activity was used to determine the changes in sperm plasma membrane integrity during storage (Gączarzewicz et al, 2015). Enzyme activity was assessed by the kinetic method according to the manufacturer's protocol (A6461, Alpha Diagnostics, Poland).…”

Section: Semen Evaluationmentioning

confidence: 99%

Bacterial Contamination of Boar Semen and its Relationship to Sperm Quality Preserved in Commercial Extender Containing Gentamicin Sulfate

Gączarzewicz¹,

Udała²,

Piasecka³

et al. 2016

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

This study was designed to determine the degree and type of bacterial contamination in boar semen (79 ejaculates from Large White and Landrace boars) and its consequences for sperm quality during storage (27 extended semen samples, 16 o C for five days) under practical conditions of artificial insemination (AI). The results revealed the presence of aerobic bacteria in 99% of the ejaculates (from 80 to 370 ×10 6 colony-forming units/mL). Most of the ejaculates contained two or three bacterial contaminants, while the Staphylococcus, Streptococcus, and Pseudomonas bacterial genera were most frequently isolated. Also detected were Enterobacter spp., Bacillus spp., Proteus spp., Escherichia coli, P. fluorescens, and P. aeruginosa. In general, the growth of certain bacterial types isolated prior to semen processing (Enterobacter spp., E. coli, P. fluorescens, and P. aeruginosa) was not discovered on different days of storage, but fluctuations (with a tendency towards increases) were found in the frequencies of Bacillus spp., Pseudomonas spp., and Staphylococcus spp. isolates up to the end of storage. Semen preserved for five days exhibited decreases in sperm motility and increases in the average number of total aerobic bacteria; this was associated with sperm agglutination, plasma membrane disruption, and acrosome damage. We inferred that, due to the different degrees and types of bacterial contaminants in the boar ejaculates, the inhibitory activity of some antimicrobial agents used in swine extenders (such as gentamicin sulfate) may be limited. Because such agents can contribute to the overgrowth of certain aerobic bacteria and a reduction in the quality of stored semen, procedures with high standards of hygiene and microbiological control should be used when processing boar semen.

show abstract

“…Interestingly, no differences between fractions were observed after the use of the SCP extender, which suggests that the choice of extender is essential. When the ejaculate fraction is diluted with an extender, the effect of seminal plasma on spermatozoa is significantly limited, and prolonged storage in an extender modifies the structural and functional characteristics of sperm cells (Garcia et al 2009, Gączarzewicz et al 2015. Our study demonstrated that the quality of stored spermatozoa is affected not only by ejaculate fraction but also significantly by the type of extender.…”

Section: Discussionmentioning

confidence: 47%

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

Dziekońska¹,

Świąder²,

Koziorowska-Gilun³

et al. 2017

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17 o C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0.05) were noted between F1 and F2 in progressive motility (PMOT), velocity average pathway (VAP) and velocity straight line (VCL). After the ejaculate was diluted, the type of fraction and type of extender significantly affected (p<0.05) PMOT, being markedly higher (p<0.05) for F1 extended in BTS. No marked changes (p<0.05) were observed between F1 and F2 extended in SCP for any of the analyzed sperm quality parameters during seven days of storage. Significantly higher (p<0.05) values of sperm quality parameters were noted in F1 compared with F2 for BTS on D7 of storage. The results of the four-way ANOVA analysis indicate that boar, fraction of ejaculate, extender type and day of storage had significant effects on the quality of boar stored spermatozoa. The F1 was characterised by higher quality of spermatozoa during storage in comparison with F2 in the short-term extender. Using the long-term extender containing the proteins allowed for a better application of F2, which could be important for the pig industry.

show abstract

Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability

Cited by 30 publications

References 58 publications

Preservation of boar semen: An update

Preservation of boar semen: An update

Bacterial Contamination of Boar Semen and its Relationship to Sperm Quality Preserved in Commercial Extender Containing Gentamicin Sulfate

Effect of boar ejaculate fraction, extender type and time of storage on quality of spermatozoa

Contact Info

Product

Resources

About