Storage-phosphor autoradiography: A rapid and highly sensitive method for spatial imaging and quantitation of radioisotopes

In vitro and ex vivo storage phosphor imaging of short-living radioisotopes

“…X-ray film autoradiography is known to be hampered by a non-linear relation between the detected density and the actual radioactive concentrations in the sample, which necessitates comparison with calibration standards or calibrated densitometry (Cross et al, 1974). For storage phosphor imaging, previous studies showed a linear response of the IPs for many radioisotopes over a wide range of radioactive concentrations Gatley et al, 1998;Gunther et al, 2000;Haaparanta et al, 2006;Kamarainen et al, 2006;Kanekal et al, 1995;Kolbe and Dietzel, 2000;Potchoiba et al, 1998;Strome et al, 2005).…”

“…After excitation with a laser beam, luminescence light is released from the energy that is stored by these crystals, which is detected by a photomultiplier tube. The latent image on the plate, which results from exposure to the radioactive source, is scanned and the amplified signal is transferred to a computer for further analysis with appropriate software Kanekal et al, 1995). The plates can then be erased and used again.…”

Small animal imaging facility: new perspectives for the radiologist

“…Alternatively, cryosection of the entire body followed by digital autoradiography [6] can provide anatomical images with very high spatial resolution (about 50 μm) correlated with a quantitative analysis of the tracer concentration. The main limitation of these ex vivo techniques is that each laboratory animal can only be analysed once during the research study, thus rendering the research expensive, time consuming and relatively imprecise given the physiological variation between specimens.…”

“…In alternativa, la criosezione dell'intero corpo seguita dall'autoradiografia digitale [6] può anche fornire immagini anatomiche con una altissima risoluzione spaziale (circa 50 micron) correlate ad un'analisi quantitativa della concentrazione di tracciante. Il punto debole di queste metodiche ex vivo consiste nella possibilità, durante una ricerca, di poter analizzare una sola volta ogni cavia, rendendo così la ricerca costosa, dispendiosa di tempo, e poco precisa considerata la variazione fisiologica interindividuale.…”

Laser Photo Chemotherapy: An Alternative Treatment for Cancer

“…Under such rationale, enhanced laser therapy by adjuvant local chemotherapy using CDDP/gel in the region of subtherapeutic energy levels was first demonstrated in an experimental model by Paiva et al (1997). Using authographic imaging method, Kanekal et al (1995) tested cisplatin radiotracer in suspension ( 195m Pt-CDDP, 4mg/ml) and gel implant form ( 195m Pt-CDDP/gel, 4mg/ml) to compare tumor retention in 600mm 3 subcutaneous tumors in experimental murine model. Intratumor CDDP/gel provided a uniform drug concentration at the margins of the tumor at 4 hours, and 80% drug retention in the tumor as opposed to rapid systemic washout of 195m Pt-CDDP in suspension after intratumor administration.…”