Storage of poultry semen

Donoghue, Ann M.; Wishart, G. J.

doi:10.1016/s0378-4320(00)00160-3

Cited by 190 publications

(116 citation statements)

References 88 publications

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“…Polge (1951), therefore, dialyzed chicken semen to reduce the glycerol concentration, and 35 live chicks were successfully produced. Since then, a number of studies of freezing of chicken semen using glycerol as a cryoprotective agent have been conducted (For review, see Donoghue and Wishart, 2000;Long, 2006;Blesbois, 2007Blesbois, , 2012.…”

Section: Semen Cryopreservationmentioning

confidence: 99%

Conservation of Avian Genetic Resources

Tajima

2013

J. Poult. Sci.

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Conservation of avian genetic resources is important, as 13.0% (1,313/10,064) of known avian species are currently categorized as endangered by the International Union for Conservation of Nature and Natural Resources (IUCN, 2012). Development of an integrated system for conservation of avian genetics is necessary; however, germplasm conservation systems developed for mammals cannot be applied directly to avians, primarily due to the unique anatomical and physiological characteristics of the avian egg. Thus an alternative strategy for conservation of oviparous species of animals must be developed. Recent technological developments for producing germline chimeras by the transfer of primordial germ cells into recipient embryos has enabled the conservation and retrieval of chicken genetic resources in their complete form. Furthermore, the possibility of propagating endangered wild birds through interspecies germline chimeras has been proposed.In the present review, the current status of avian genetic resource conservation is presented with a focus on the following 4 topics: 1) semen cryopreservation, 2) germline chimeras, 3) somatic nuclear transfer, and 4) other related technologies.

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Section: Semen Cryopreservationmentioning

confidence: 99%

Conservation of Avian Genetic Resources

Tajima

2013

J. Poult. Sci.

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“…polyvinyl-pyrolidone), rapid or slow freezing/thawing rates and pellet or straw packaging. A 'historical' picture of the progress and still unanswered questions concerning avian sperm cryopreservation can be obtained from several reviews published in the last few years by Hammerstedt (1995), Surai and Wishart (1996), Donoghue and Wishart (2000), Massip et al (2004) and Blesbois (2007). Briefly, several workable methods are currently available for use in the chicken with different internal cryoprotectants (Figure 3), while results in other species remain inconsistent or insufficient.…”

Section: Semen Cryopreservationmentioning

confidence: 99%

Specific features of in vivo and in vitro sperm storage in birds

Blesbois

Brillard²

2007

Animal

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This review focuses on some of the main features of sperm selection and storage in birds mainly on the basis of studies performed in poultry species, with emphasis on the initial selection of sperm at the female vagina level prior to migration towards the sperm storage tubules. Sperm originating from low-quality males or subjected to inappropriate in vitro storage conditions are rapidly discarded, resulting in impaired fertility in corresponding flocks. In the absence of accessible and appropriate technology for matching the 'storing' potential of sperm in the oviduct, conditions for prolonged sperm storage under a liquid (through the use of semen extenders) or a solid state (cryopreservation) have received only limited attention, despite their potential interest to facilitate male and female management in poultry flocks. Despite this, technology for shortterm liquid storage is currently used in turkeys, guinea fowl and muscovy ducks and also in progress in chickens. In addition, technology for cryopreservation of avian semen has become available for some species (chicken, goose) to facilitate the management of genetic resources, including the preservation of rare and economically important breeds.

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“…However, fertility rates of cryopreserved poultry semen remain highly variable and are not reliable for use in commercial production or the preservation of genetic resources in cryobanks. Most attempts to improve the deteriorated fertility rates of cryopreserved poultry semen have focused on empirical approaches, such as the types of cryoprotectants and extenders used, freezing rate, and methods of cryopreservation (i.e., pellets and straws) (Donoghuea and Wishart, 2000). However, limited attention has been given to understand why poultry sperm lose their fertilizing ability after cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

“…The success of sperm cryopreservation is generally evaluated based on motility or plasma membrane integrity; the survivability of sperm is usually 40-50% of the initial population (Donoghuea and Wishart, 2000). However, a previous study demonstrated that the in vivo fertilizing ability of cryopreserved chicken sperm decreased to 1.6% of that of fresh sperm (Wishart, 1985).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken

et al. 2016

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Cryopreserved sperm undergoes serious damage which affects its fertilizing ability. Despite progress in understanding the nature of functional deterioration in mammalian sperm, little is known about the mechanism involved in the induction of functional damage in avian sperm. Cellular membranes are considered the primary site of cryodamage to sperm. Membrane rafts are specific membrane regions enriched in sterols, ganglioside G M1 , and functional proteins and they play important roles in the regulation of diverse functions exerted in mammalian sperm during fertilization. Several reports investigating cryopreservation-induced membrane changes in mammalian sperm have suggested that cryopreservation induces a compositional alteration of membrane rafts via a loss of membrane sterols, leading to impaired fertilizing ability. Recently, we demonstrated that membrane rafts are present in chicken sperm. Therefore, we investigated a possible mechanism for the induction of functional damage in cryopreserved chicken sperm, with particular attention to cryopreservation-induced compositional changes in membrane rafts. Sterol quantification showed that loss of sterols from sperm membranes occurred following cryopreservation. Biochemical analyses of detergent-insoluble membranes showed that the lipid and protein compositions of membrane rafts were altered dramatically by cryopreservation. To determine the physiological role of these changes, we examined external translocation of phosphatidylserine (PS), representing an early apoptotic change, and found that cryopreservation induced apoptotic changes in chicken sperm. Furthermore, methyl-β-cyclodextrin-induced loss of sterols from the plasma membranes stimulated PS translocation that was not accompanied with caspase-3 activation, which plays an important role downstream of the apoptotic cascade. Based on the results obtained in this study, we discuss a new mechanism for reduction of the fertilizing ability in avian sperm after cryopreservation.

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Storage of poultry semen

Cited by 190 publications

References 88 publications

Conservation of Avian Genetic Resources

Conservation of Avian Genetic Resources

Specific features of in vivo and in vitro sperm storage in birds

Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken

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