Storage elicits a fast antioxidant enzyme activity in Araucaria angustifolia embryos

Araldi, Cristhyane Garcia; Coelho, Cileide Maria Medeiros; Gaziola, Salete Aparecida; Azevedo, Ricardo Antunes

doi:10.1007/s11738-016-2219-2

Cited by 9 publications

(6 citation statements)

References 58 publications

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“…On the other hand, the decreased expression of the CAT2 , MDAR5 , ACT1 , LEA and PDIL1-4 genes in the NR genotype at 30, 90 and 150 doi may have contributed to the high levels of oxidation that may be one of the causes for the absence of callus formation in this genotype. According to Araldi et al [51] and Zhou et al [52], catalase is an antioxidant protein that acts on the regulation of ROS, and its reduced activity results in the accumulation of hydrogen peroxide (H 2 O 2 ). The activity of catalase in scavenging H 2 O 2 was associated with the embryogenic potential of the calli of Mesembryanthemum crystallinum [49] and Larix leptolepis [53] during SE.…”

Section: Resultsmentioning

confidence: 99%

Genotype-dependent changes of gene expression during somatic embryogenesis in oil palm hybrids (Elaeis oleifera x E. guineensis)

et al. 2018

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To understand the molecular processes triggered during the different steps of somatic embryogenesis (SE) in oil palm, the expression of 19 genes associated to SE identified in proteomic and transcriptomic studies was investigated by qRT-PCR. To evaluate the differential expression of these genes, two interspecific hybrid genotypes (Elaeis oleifera x Elaeis guineensis) contrasting for the acquisition of embryogenic competence were used. Aclorophyllated leaves of both hybrids, one responsive (B351733) and the other non-responsive (B352933) to SE were submitted to callus induction and collected at different time points: 0 (before induction), 14, 30, 90 and 150 days of callus induction (doi). The results obtained showed that all evaluated genes were downregulated at 14 doi in the responsive genotype when compared to the non-responsive. It was also possible to observe that most of the genes changed their expression behavior at 30 doi and were upregulated thereafter until 150 doi, with the exception of the pathogenesis-related PRB1-3-like (PRB1-3) gene, which did not show differential expression at 30 doi and was downregulated at 90 and 150 doi when compared to the non-responsive hybrid. These results indicate that 30 doi is a turning point in gene expression, probably associated to embryogenic competence acquisition. We also show that the expression behavior of the responsive genotype is more stable than that of the non-responsive when the different induction time points are compared to 0 doi (before induction). Moreover, the results obtained in this study corroborate our hypothesis that the regulation of genes involved in the control of oxidative stress and energy metabolism are crucial for the acquisition of embryogenic competence in oil palm.

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Section: Resultsmentioning

confidence: 99%

Genotype-dependent changes of gene expression during somatic embryogenesis in oil palm hybrids (Elaeis oleifera x E. guineensis)

et al. 2018

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“…Kuntze) are responsible for a significant portion of the economy of southern Brazil, and the bio-residues generated during the preparation of pinhão seeds are frequently used in medicine, mainly in the treatment of respiratory diseases. 11,12 Pinhão seeds are consumed after baking or cooking in water. Both the residual coat (external tegument or shell) and the water extract that results from the cooking process are rich in phenolic compounds and can be explored as bio-residues.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity screening of pinhão (Araucaria Angustifolia (Bertol.) Kuntze) seed extracts: the inhibition of cholinesterases and α-amylases, and cytotoxic and anti-inflammatory activities

et al. 2021

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“…The activity of ROS scavenging system of seeds was closely related to seed recalcitrance production [47][48][49]. The activity of antioxidant enzymes of S. tonkinensis seeds was enhanced after 0~8 h-dehydration and cryopreservation (Figure 3), indicating that plant defense system generated self-help measures to enhance the activity of antioxidant enzymes to fight against ROS free radicals produced by dehydration and low temperature stress.…”

Section: Molecular Mechanism Of Dehydration Tolerance In S Tonkinensi...mentioning

confidence: 99%

iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds

Luo

Zhang

Jiang

et al. 2023

Plants

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Sophora tonkinensi is a shrub of the genus Sophora in the family Fabaceae with anti-inflammatory and pain-relieving effects. While the cultivation, chemical makeup, and medicinal properties of S. tonkinensis have been reported, the physiological mechanisms governing its dehydration and cryopreservation tolerance of seeds remain unclear. In this study, we investigated the morphological, physiological, biochemical, and protein expression characteristics of S. tonkinensis seeds subjected to dehydration and cryopreservation techniques via the observation of cell microstructure, determination of antioxidant enzyme activity, and iTRAQ-based proteomic analysis, respectively. The results of the study demonstrated that the seeds possessed a certain level of tolerance to dehydration. The highest germination percentage of 83.0% was observed after 2 h of dehydration (10.1% water content), which was identified as the optimal time point for cryopreservation. However, the germination percentage was reduced to only 30.5% when the water content reached 5.4%, indicating that S. tonkinensis seeds exhibit intermediate storage behavior. Further investigation revealed that during seed dehydration and cryopreservation treatment, liposomes were gradually and highly fused, whereas the activities of ROS scavenging and stress defense were significantly enhanced. During dehydration, the seed tissues formed a protective mechanism of stress resistance based on protein processing in the endoplasmic reticulum and antioxidant system, which was related to the dehydration tolerance. Moreover, only three differentially expressed LEA proteins were identified, and it is speculated that the strengthening of intracellular metabolism and the absence of specific LEA and dehydrins could be crucial factors for the reduced germination percentage after excessive dehydration and cryopreservation.

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Storage elicits a fast antioxidant enzyme activity in Araucaria angustifolia embryos

Cited by 9 publications

References 58 publications

Genotype-dependent changes of gene expression during somatic embryogenesis in oil palm hybrids (Elaeis oleifera x E. guineensis)

Genotype-dependent changes of gene expression during somatic embryogenesis in oil palm hybrids (Elaeis oleifera x E. guineensis)

Bioactivity screening of pinhão (Araucaria Angustifolia (Bertol.) Kuntze) seed extracts: the inhibition of cholinesterases and α-amylases, and cytotoxic and anti-inflammatory activities

iTRAQ-Based Proteomic and Physiological Analyses Reveal the Mechanisms of Dehydration and Cryopreservation Tolerance of Sophora tonkinensis Gagnep. Seeds

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