Stool Short-Chain Fatty Acids in Critically Ill Patients with Sepsis

Abstract

Objective: To determine the concentration of stool short-chain fatty acids (SCFAs) in critically ill patients with sepsis and to compare the results between the critically ill patient and the control group. Methods: This descriptive, multicenter, observational study was conducted in five health institutions. Over a 6-month study period, critically ill patients with sepsis who were admitted to the intensive care unit (ICU) and met the inclusion criteria were enrolled, and a control, paired by age and sex, was recruited for each patient. A spontaneous stool sample was collected from each participant and a gas chromatograph coupled to a mass spectrometer (Agilent 7890/MSD 5975 C) was used to measure the concentrations SCFAs. Results: The final sample included 44 patients and 45 controls. There were no differences in the age and sex distributions between the groups (p > 0.05). According to body mass index (BMI), undernutrition was more prevalent among critically ill patients, and BMI in control subjects was most frequently classified as overweight (p ¼ 0.024). Propionic acid, acetic acid, butyric acid, and isobutyric acid concentrations were significantly lower in the critically ill patient group than in the control group (p ¼ 0.000). No association with outcome variables (complications, ICU stay, and discharge condition) was found in the patients, and patients diagnosed with infection on ICU admission showed significant decreases in butyric and isobutyric acid concentrations with respect to other diagnostic criteria (p < 0.05). Conclusions:The results confirm significantly lower concentrations of stool SCFAs in critically ill patients with sepsis than in control subjects. Due to its role in intestinal integrity, barrier function, and anti-inflammatory effect, maintaining the concentration of SCFAs may be important in the ICU care protocols of the critical patient.

Dynamic change of fecal microbiota and metabolomics in a polymicrobial murine sepsis model

“… 21 The concentrations of SCFAs were reported to be significantly lower in critically ill patients than in healthy volunteers. 22 , 23 The amounts of SCFAs continued low for more than 6 weeks 24 and were associated with mortality. 25 In our study, the concentrations of SCFAs such as isovaleric acid, butyric acid, and propionic acid decreased.…”

Gut Microbiota Mediates the Susceptibility of Mice to Sepsis-Associated Encephalopathy by Butyric Acid

“…Studies have shown that short chain fatty acids (SCFAs), one of the most important metabolites of gut microbiota, its concentration in the feces of sepsis patients is significantly reduced and affected the physiological function of the body in sepsis. 9 , 10 SCFAs can improve cerebral damage by the blood-brain barrier (BBB). 11 The microbiota is a vital regulator of gut-brain function, and the importance of the microbiota-gut-brain axis is becoming more widely recognized.…”

Nutritional Status and the Critically Ill Patient: Gut Microbiota and Immuno-Nutrition in I.C.U. at the Time of SARS-COV 2 Pandemic

“…However, no significant association with complications occurrence, ICU length of stay and discharge conditions was found. Indeed, ICU patients diagnosed with an infection upon admission showed significantly decreased levels of butyric and isobutyric acids vs. other groups (p < 0.05) [27]. Very interestingly, another action of prebiotics on gut microbiota modulation is resembled by the anti-inflammatory effect of their administration.…”