Stomatobaculum longum gen. nov., sp. nov., an obligately anaerobic bacterium from the human oral cavity

Sizova, Maria V.; Muller, Paul; Panikov, N. S.; Mandalakis, Manolis; Hohmann, Tine; Hazen, Amanda; Fowle, William H.; Prozorov, Tanya; Bazylinski, Dennis A.; Epstein, Slava S.

doi:10.1099/ijs.0.042812-0

Cited by 40 publications

(27 citation statements)

References 26 publications

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“…In agreement, decreased Lachnospiraceae were previously described in oral microbiome from obese compared to non-obese type 2 diabetic individuals [40]. Characterization of Stomatobaculum and Oribacterium asaccharolyticum, highlighted that Stomatobaculum encoded in its genome the cysteine desulfurase gene [41], whereas the major metabolic end product of Oribacterium asaccharolyticum was acetate [42]. Interestingly, a recent study evaluated the associations of BMI with circulating microbiota biomarkers in African American men and found that propionic and butyric SCFAs, but not acetic acid, were significant positive predictors of BMI [43].…”

Section: Discussionsupporting

confidence: 81%

Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing

et al. 2020

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The gut microbiota may have an impact on obesity. To date, the majority of studies in obese patients reported microbiota composition in stool samples. The aim of this study was to investigate the duodenal mucosa dysbiosis in adult obese individuals from Campania, a region in Italy with a very high percentage of obese people, to highlight microbial taxa likely associated with obesity. Duodenum biopsies were taken during upper gastrointestinal endoscopy in 19 obese (OB) and 16 lean control subjects (CO) and microbiome studied by 16S rRNA gene sequencing. Duodenal microbiome in our groups consisted of six phyla: Proteobacteria, Firmicutes, Actinobacteria, Fusobacteria, Bacteroidetes and Acidobacteria. Proteobacteria (51.1% vs. 40.1%) and Firmicutes (33.6% vs. 44.9%) were significantly (p < 0.05) more and less abundant in OB compared with CO, respectively. Oribacterium asaccharolyticum, Atopobium parvulum and Fusobacterium nucleatum were reduced (p < 0.01) and Pseudomonadales were increased (p < 0.05) in OB compared with CO. Receiver operating characteristic curve analysis showed Atopobium and Oribacterium genera able to discriminate with accuracy (power = 75% and 78%, respectively) OB from CO. In conclusion, increased Proteobacteria and decreased Firmicutes (Lachnospiraceae) characterized the duodenal microbiome of obese subjects. These data direct to further studies to evaluate the functional role of the dysbiotic-obese-associated signature.

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Section: Discussionsupporting

confidence: 81%

Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing

et al. 2020

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“…Other highly acidogenic, lactate-producing species in dentin included A. rimae and A. parvulum [37] although the acid tolerance of these species is unknown. Other less acidogenic species with genes detected in dentin, included Prevotella and Capnocytophaga species and S. longum [38], likely reflecting the higher pH deeper in dentin compared to the tooth surface, and proteolytic activity related to dentin caries [10]. Detection of genes in dentin mapping to A. oris, P. denticola, A. parvulum and the genus Rothia in dentin was as previously reported [11].…”

Section: Discussionmentioning

confidence: 53%

Functional profiles of coronal and dentin caries in children

Kressirer

Chen

Harriman³

et al. 2018

Journal of Oral Microbiology

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Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition.Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compared with health.Design: Samples from children, caries-free (CF: n = 4) or with coronal (CC: n = 5) or dentin (DC: n = 5) caries were examined for gene expression potential. Functional profiling was performed using HUMAnN2 (HMP Unified Metabolic Analysis Network).Results: There was increased gene expression diversity in DC compared with CC and CF. Genes in CF included alcohol dehydrogenase from Neisseria sicca, methylenetetrahydrofolate reductase from Streptococcus sanguinis and choline kinase from streptococci. Genes in CC mapped mainly to Streptococcus mutans. Arginine deiminase in DC mapped to S. sanguinis and Actinomyces naeslundii. Glycerol kinase genes mapped to S. sanguinis in all groups whereas glycerol kinase in DC were from Rothia, Prevotella and streptococci. Uracil-DNA glycosylase in DC mapped to Prevotella denticola and Actinomyces. Repressor LexA in DC mapped to Scardovia wiggsiae, Dialister invisus and Veillonella parvula.Conclusions: Functional profiling revealed enzyme activities in both caries and caries-free communities and clarified marked differences between coronal and dentin caries in bacterial composition and potential gene expression.

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“…Most cells of strain ACB7 T and some cells of strain ACB1 T contained intracellular nanometre-sized particles ( Fig. 1), probably of ferrous sulfide (Sizova et al, 2013). Upon inspection of the genomes of strains ACB1 T , ACB8 and ACB7 T , we identified five to six genes putatively annotated as encoding the cysteine desulfurase enzyme EC 2.8.1.7 (Table S1).…”

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confidence: 99%

“…Cell morphology was observed with a Leica DMBL light microscope equipped with phase-contrast optics. For electron microscopy, cells grown on TY medium for 24-48 h were collected, fixed as described previously (Ellis, 2006;Sizova et al, 2013) and observed with a Hitachi S4800 scanning electron microscope. Thin sections were stained with uranyl acetate and lead citrate and observed with a JEOL JEM 1010 transmission electron microscope.…”

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confidence: 99%

Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium

Sizova

Muller

Stancyk

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1 T , ACB7 T and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1 T and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7 T grew weakly on sucrose only. The growth temperature range was 30-42 6C with optimum growth at 37 6C. Major metabolic fermentation end products of strain ACB1 T were acetate and lactate; the only product of strains ACB7 T and ACB8 was acetate. Major fatty acids of strain ACB1 T were C 14 : 0 , C 16 : 0 , C 16 : 1 v7c dimethyl aldehyde (DMA) and C 18 : 1 v7c DMA. Major fatty acids of strain ACB7 T were C 12 : 0 , C 14 : 0 , C 16 : 0 , C 16 : 1 v7c and C 16 : 1 v7c DMA. The hydrolysate of the peptidoglycan contained meso-diaminopimelic acid, indicating peptidoglycan type A1c. Genomic DNA G+C content varied from 42 to 43.3 % between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1 T , ACB8 and ACB7 T formed two separate branches within the genus Oribacterium, with 98.1-98.6 % sequence similarity to the type strain of the type species, Oribacterium sinus. Predicted DNA-DNA hybridization values between strains ACB1 T , ACB8, ACB7 T and O. sinus F0268 were ,70 %. Based on distinct genotypic and phenotypic characteristics, strains ACB1 T and ACB8, and strain ACB7 T are considered to represent two distinct species of the genus Oribacterium, for which the names Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov. are proposed. The type strains are ACB1 T (5DSM

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Stomatobaculum longum gen. nov., sp. nov., an obligately anaerobic bacterium from the human oral cavity

Abstract: Stomatobaculum longum gen. nov., sp. nov., an obligately anaerobic bacterium from the human oral cavity

Cited by 40 publications

References 26 publications

Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing

Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing

Functional profiles of coronal and dentin caries in children

Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium

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