Stochastic Collision Electrochemistry from Single G-Quadruplex/Hemin: Electrochemical Amplification and MicroRNA Sensing

Abstract

Stochastic collision electrochemistry is a hot topic in single molecule/particle research, which provides an opportunity to investigate the details of the single molecule/particle reaction mechanism that is always masked in ensemble-averaged measurements. In this work, we develop an electrochemical amplification strategy to monitor the electrocatalytic behavior of single G-quadruplex/hemin (GQH) for the reaction between hydrogen peroxide and hydroquinone (HQ) through the collision upon a gold nanoelectrode. The intrinsic peroxidase activities of single GQH were investigated by stochastic collision electrochemical measurements, giving further insights into understanding biocatalytic processes. Based on the unique catalytic activity of GQH, we have also designed a hybridization chain reaction strategy to detect miRNA-15 with good selectivity and sensitivity. This work provided a meaningful strategy to investigate the electrochemical amplification and the broad application for nucleic acid sensing at the single molecule/particle level.

Identification and targeting of G-quadruplex structures in MALAT1 long non-coding RNA

“…G4 prediction analysis including cGcC (Consecutive G over consecutive C ratio) ( 56 ), G4H (G4Hunter) ( 57 ) and G4NN (G4 Neural Network) ( 58 ) was carried out by G4RNA screener v.0.2 ( 59 ) ( http://scottgroup.med.usherbrooke.ca/G4RNA_screener/ ) with default setting. The G4 threshold is defined as cGcC >4.5, G4H >0.9, G4NN >0.5.…”

A general controllable release amplification strategy of liposomes for single-particle collision electrochemical biosensing

“…The commonly used quantitative method based on the standard curve between target concentrations and signal intensity was no longer applicable. Therefore, based on SPCE, collision frequency instead of current intensity was used for analytical detection, which was expected to avoid the problem of electrochemical signal fluctuations, demonstrating the application advantages of SPCE in analytical detection ( Castaneda et al, 2017 ; Defnet and Zhang, 2021 ; Wang et al, 2021a ).…”

“…Bai et al applied the similar method and combined duplex-specific nuclease-mediated cycle amplification to achieve more sensitive detection of miRNA-21 ( Bai et al, 2020 ). Signal amplification strategies such as hybridization chain reaction, chain replacement, enzyme-catalyzed reaction have been very mature for DNA/RNA detection ( Castaneda et al, 2017 ; Wang et al, 2021a , Wang et al, 2021b ). Therefore, it is interesting to combine these signal amplification methods with SPCE to increase collision frequency and detection sensitivity.…”

Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing

“…Thus, false positive can be well controlled with high sensitivity and selectivity . HCR shows great potential compared with not only commercial PCR but also other techniques including rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), catalyzed hairpin assembly (CHA), ligase/helicase-dependent amplification, , and exonuclease/endonuclease-assisted amplification. , Currently, HCR has been widely utilized in the measurement of low abundant targets such as small molecules, DNA/RNA, peptides/proteins, bacteria, and cells (Table ). Most reviews about HCR focused on traditional linear HCR assembly, , a few discussed novel forms of HCR .…”