Stochastic Biosensing by a Single Protein Nanopore in the Development of Analytical Tools

Aguiar, Juliana Pereira de; Júnior, Janilson J. S.; Machado, Dijanah Cota; Melo, Maria C. A.; Rodrigues, Cláudio Gabriel

doi:10.5935/0100-4042.20150073

Cited by 3 publications

(2 citation statements)

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“…Os biossensores são dispositivos analíticos que tem seu uso direcionado para a detecção de substancias-alvo (analito), combinando um detector físico-químico e um componente biológico, estes métodos são enquadrados como tecnologia biofísica. Os biossensores funcionam através da interação do analito com o elemento sensor isto, gera um sinal que é transformado e processado até que se torne passível de interpretação (AGUIAR, et al 2015). Essa metodologia vem sendo muito difundida na ciência, pois possui entre outras características, a facilidade de uso (reduzindo os requisitos para pessoal treinado), custos mais baixos, alta sensibilidade, plataformas robustas e boa reprodutibilidade (BERTANI & LU, 2021).…”

Section: Biossensoresunclassified

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

Rodrigues

De-Carli

Vicentin

et al. 2021

Bol. Lab. Hidrobiol.

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Muitos corpos hídricos têm sido degradados devido à interferência humana. A poluição causada pelas atividades antrópicas vem comprometendo a qualidade da água e promovendo eventos de eutrofização, afetando toda estrutura trófica do ambiente, podendo assim acarretar na proliferação de cianobactérias tóxicas. Nesse sentido, o conhecimento sobre esses organismos é de grande importância, principalmente em corpos hídricos com usos múltiplos. Esta pesquisa representa uma revisão de trabalhos publicados envolvendo qualidade ambiental e ecolotoxicologia aquática, tendo como foco estudos relacionados às cianobactérias e cianotoxinas. Para o trabalho foram analisados livros textos e artigos publicados entre 2010 e 2020 em periódicos nacionais e internacionais. Na literatura publicada nos últimos anos são apresentadas as principais metodologias envolvidas na detecção e remoção de cianotoxinas, além de bioensaios ecotoxicológicos. Fica evidente a importância do tratamento de efluentes e controle das fontes de poluição difusas e pontuais no ecossistema promovendo a melhoria da qualidade da água e controle de cianobactérias.ABSTRACTCyanobacteria play an important role among producers in aquatic ecosystems, however when associated with eutrophication they become an ecological and public health problem. The problem involving cyanobacteria is the blooming of potentially toxic strains. The present work aims to present and discuss the problems related to the proliferation of cyanobacteria in limnological environments, and its consequences for dynamics in the environment and effects on environmental health. In this sense, knowledge about these organisms is of great importance, especially in water bodies with multiple uses. This research represents a review of published works involving environmental quality and aquatic ecotoxicology, focusing on studies related to cyanobacteria and cyanotoxins. For the present work, textbooks and articles published between 2010 and 2020 in national and internationaljournals were analyzed. In the literature published in recent years, the main methodologies involved in the detection and removal of cyanotoxins are presented, in addition to ecotoxicological bioassays. The study of cyanobacteria and their metabolites comprises an area of interest that reaches a greater dimension when impacts on the quality of water supply are evident, thus the importance of treating effluents and controlling pollution sources in the ecosystem is evident, promoting improvement and maintenance the quality of the water bodies.

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Section: Biossensoresunclassified

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

Rodrigues

De-Carli

Vicentin

et al. 2021

Bol. Lab. Hidrobiol.

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“…The interaction between the analyte and the unitary nanopore induces blockage events in the ionic current. The time series formed by these blockage events generates a digital signature that allows the identification and quantification of the analyte [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Alphatoxin Nanopore Detection of Aflatoxin, Ochratoxin and Fumonisin in Aqueous Solution

Silva

Júnior

Cavalcanti

et al. 2023

Toxins

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Mycotoxins are toxic and carcinogenic metabolites produced by groups of filamentous fungi that colonize food crops. Aflatoxin B1 (AFB1), ochratoxin A (OTA) and fumonisin B1 (FB1) are among the most relevant agricultural mycotoxins, as they can induce various toxic processes in humans and animals. To detect AFB1, OTA and FB1 in the most varied matrices, chromatographic and immunological methods are primarily used; however, these techniques are time-consuming and expensive. In this study, we demonstrate that unitary alphatoxin nanopore can be used to detect and differentiate these mycotoxins in aqueous solution. The presence of AFB1, OTA or FB1 inside the nanopore induces reversible blockage of the ionic current flowing through the nanopore, with distinct characteristics of blockage that are unique to each of the three toxins. The process of discrimination is based on the residual current ratio calculation and analysis of the residence time of each mycotoxin inside the unitary nanopore. Using a single alphatoxin nanopore, the mycotoxins could be detected at the nanomolar level, indicating that alphatoxin nanopore is a promising molecular tool for discriminatory analysis of mycotoxins in aqueous solution.

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O NANOPORO PROTEICO UNITÁRIO DA Α-Hemolisina DETECTA POLIVINILÁLCOOL?

Xavier¹,

Rodrigues²,

Aguiar³

2017

Blucher Biophysics Proceedings

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Stochastic Biosensing by a Single Protein Nanopore in the Development of Analytical Tools

Cited by 3 publications

References 0 publications

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

Alphatoxin Nanopore Detection of Aflatoxin, Ochratoxin and Fumonisin in Aqueous Solution

O NANOPORO PROTEICO UNITÁRIO DA Α-Hemolisina DETECTA POLIVINILÁLCOOL?

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