Stirred-type yoghurt incorporated with sour cherry extract in chitosan-coated liposomes

Akgün, Dila; Gültekin-Özgüven, Mine; Yücetepe, Aysun; Altın, Gökçe; Gibis, Monika; Weiß, Jochen; Özçelik, Beraat

doi:10.1016/j.foodhyd.2019.105532

Cited by 52 publications

(18 citation statements)

References 23 publications

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“…The zeta potential was highly negative for all the extract-loaded vesicles (~−48 mV, p > 0.05 among all) irrespective of their composition. The results are in agreement with other studies, which confirmed the negative charge of liposomes made with the zwitterionic phosphatidylcholine [ 39 , 44 ]. The amount of extract incorporated into the vesicles was very high (entrapment efficiency ~89%) without significant differences among the different samples.…”

Section: Resultssupporting

confidence: 93%

Extraction, Characterization and Incorporation of Hypericum scruglii Extract in Ad Hoc Formulated Phospholipid Vesicles Designed for the Treatment of Skin Diseases Connected with Oxidative Stress

et al. 2020

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An extract of Hypericum scruglii, an endangered endemic plant of Sardinia (Italy), was prepared and characterized. It was loaded in special phospholipid vesicles, glycerosomes, which were modified by adding maltodextrin (glucidex) and a polymer (gelatin or hyaluronan). The corresponding liposomes were also prepared and used as reference. The vesicles disclosed suitable physicochemical features for skin delivery. Indeed, their mean diameter ranged from 120 to 160 nm, they were homogeneously dispersed (polydispersity index ≤ 0.30), and their zeta potential was highly negative (~−45 mV). The vesicle dispersions maintained unchanged characteristics during 60 days of storage, were highly biocompatible, and were able to protect keratinocytes against damages due to oxidative stress induced by treating them with hydrogen peroxide. Vesicles were also capable of promoting cell proliferation and migration in vitro by means of a scratch wound assay. The results confirmed the fruitful delivery of the extract of H. scruglii in glycerosomes modified with glucidex and gelatin and their promising ability for skin protection and treatment.

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Section: Resultssupporting

confidence: 93%

Extraction, Characterization and Incorporation of Hypericum scruglii Extract in Ad Hoc Formulated Phospholipid Vesicles Designed for the Treatment of Skin Diseases Connected with Oxidative Stress

et al. 2020

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“…In other words, the conversion of proliposomes to liposomes did not affect the encapsulation process. In fact, the EE results are comparable with our previous studies ( Altin et al., 2018b ; Akgün et al., 2020 ). While the formulations with 0.1% (w/v) of PPE resulted in the highest encapsulation efficiency, 0.2% (w/v) concentration of PPE was selected to make the spectrophotometric measurements easier due to multiple dilutions in the further steps.…”

Section: Resultssupporting

confidence: 91%

“…The increment of particle size and alteration of the surface charge of the liposomes are two important parameters for successful surface coating. In our previous studies, we have reported that the introduction of the negatively charged phenolic extract to the anionic liposomal dispersion increase the negativity of the total zeta potential of liposomal dispersions ( Gültekin-Özgüven et al., 2016 ; Altin et al., 2018b ; Akgün et al., 2020 ). However, the surface charge of phenolic pollen extract in pH between 3.0-3.5 has been found as neutral ( Sebii et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…In several studies ( Akgün et al., 2020 ; Altin et al., 2018b ; Gibis et al., 2014 ; Gültekin-Özgüven et al., 2016 ), it has been indicated that the optimum encapsulation efficiency with liposomes can be reached using 0.1–0.2% (w/v) of phenolic concentration. To compare the encapsulation ability of P-liposomes respect to the C-liposomes in terms of optimum encapsulation efficiency, PPEs with a concentration of 0.1 and 0.2% (w/v) were studied.…”

Section: Resultsmentioning

confidence: 99%

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Liposomal nanodelivery systems generated from proliposomes for pollen extract with improved solubility and in vitro bioaccessibility

Hızır-Kadı

Gültekin-Özgüven

Altın

et al. 2020

Heliyon

Self Cite

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This study offers a suitable and easy proliposome-liposome method that enhances the encapsulation ability of liposome structures on poor water-soluble extracts. Pollen phenolic extract (PPE) was studied to show applicability in the proposed method. The poor water-soluble PPE (0.2%, w/v) was encapsulated by liposomes generated from proliposomes (P-liposomes) that were prepared via high-pressure homogenization technique without using any organic solvents and high temperature. Only a few drops of ethanol were used to dissolve poor water-soluble compounds in PPE during the preparation of P-liposomes. The trace amount of ethanol maintained the improvement of PPE solubility in P-liposome dispersion, hence the in vitro bioaccessibility and bioactivity of PPE incorporated in P-liposomes increased. Thus, higher encapsulation efficiency was found in P-liposomes compared to conventional liposomes (C-liposomes) in which the EE was 75 and 73%, respectively. To increase the physical stability of liposome structures, the surface of both P-liposomes and C-liposomes was covered with chitosan. There were found small changes between P-liposomes and C-liposomes in terms of mean diameter size and zeta potential. On the other hand, the bioactivity of encapsulated PPE showed differences in P-liposomes and C-liposomes. The antioxidant capacity of PPE in P-liposomes enhanced approximately two times in CUPRAC and three times in DPPH assays. Also, in vitro bioaccessibility of PPE in P-liposomes increased approximately 4 and 2 folds, respectively, regarding total phenolics and flavonoids. To our knowledge, this is the first report about the increment of encapsulation behavior of liposome structures on low water-soluble extract within an aqueous media.

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“…For this reason, transforming the aqueous liposomal dispersion into powder means creating a release system that is more fit for industrial production. This was the goal of Akgün et al [126], who showed a promising industrially applicable delivery system for sour cherry phenols that were efficiently loaded in a liposomal powder incorporated into a stirred-type yoghurt system. Since the spray-drying process did not degrade phenolic compounds encapsulated in liposomes, this technique could represent another strategy for reducing polyphenols degradation and enhancing their beneficial activity.…”

Section: Intestinal Absorptionmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Properties of Cherry Extract: Nanosystems-Based Strategies to Improve Endothelial Function and Intestinal Absorption

Beconcini

Felice

Fabiano

et al. 2020

Foods

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Cherry fruit has a high content in flavonoids. These are important diet components protecting against oxidative stress, inflammation, and endothelial dysfunction, which are all involved in the pathogenesis of atherosclerosis, which is the major cause of cardiovascular diseases (CVD). Since the seasonal availability of fresh fruit is limited, research has been focused on cherry extract (CE), which also possesses a high nutraceutical potential. Many clinical studies have demonstrated the nutraceutical efficacy of fresh cherries, but only a few studies on CE antioxidant and anti-inflammatory activities have been carried out. Here, the results concerning the antioxidant and anti-inflammatory activities of CE are reviewed. These were obtained by an in vitro model based on Human Umbilical Vein Endothelial Cells (HUVEC). To clarify the CE mechanism of action, cells were stressed to induce inflammation and endothelial dysfunction. Considering that antioxidants’ polyphenol compounds are easily degraded in the gastrointestinal tract, recent strategies to reduce the degradation and improve the bioavailability of CE are also presented and discussed. In particular, we report on results obtained with nanoparticles (NP) based on chitosan derivatives (Ch-der), which improved the mucoadhesive properties of the chitosan polymers, as well as their positive charge, to favor high cellular interaction and polyphenols intestinal absorption, compared with a non-mucoadhesive negative surface charged poly(lactic-co-glycolic) acid NP. The advantages and safety of different nanosystems loaded with natural CE or other nutraceuticals are also discussed.

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Stirred-type yoghurt incorporated with sour cherry extract in chitosan-coated liposomes

Cited by 52 publications

References 23 publications

Extraction, Characterization and Incorporation of Hypericum scruglii Extract in Ad Hoc Formulated Phospholipid Vesicles Designed for the Treatment of Skin Diseases Connected with Oxidative Stress

Extraction, Characterization and Incorporation of Hypericum scruglii Extract in Ad Hoc Formulated Phospholipid Vesicles Designed for the Treatment of Skin Diseases Connected with Oxidative Stress

Liposomal nanodelivery systems generated from proliposomes for pollen extract with improved solubility and in vitro bioaccessibility

Antioxidant and Anti-Inflammatory Properties of Cherry Extract: Nanosystems-Based Strategies to Improve Endothelial Function and Intestinal Absorption

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