Stimuli-Responsive Surfaces Using Polyampholyte Polymer Brushes Prepared via Atom Transfer Radical Polymerization

Ayres, Neil; Cyrus, Crystal; Brittain, William J.

doi:10.1021/la062417+

Cited by 74 publications

(76 citation statements)

References 45 publications

(55 reference statements)

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“…Notably, this material is recombinantly expressed, forms brushes, and yields a nearly six to tenfold dynamic range in brush thickness as ionic strength is varied and a threefold range as pH is varied. This dynamic range is comparable to many existing synthetic polymer brush systems and suggests that these materials may serve as an attractive complement or alternative to synthetic systems in specific settings 24,39 . It is also conceivable that this dynamic range could be shifted, widened, or narrowed in interesting ways by exerting more control over the grafting density, such as by using cloud point grafting to achieve very dense brushes 40,41 .…”

Section: Discussionmentioning

confidence: 54%

Stimuli-sensitive intrinsically disordered protein brushes

et al. 2014

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Grafting polymers onto surfaces at high density to yield polymer brush coatings is a widely employed strategy to reduce biofouling and interfacial friction. These brushes almost universally feature synthetic polymers, which are often heterogeneous and do not readily allow incorporation of chemical functionalities at precise sites along the constituent chains. To complement these synthetic systems, we introduce a biomimetic, recombinant intrinsically disordered protein that can assemble into an environment-sensitive brush. This macromolecule adopts an extended conformation and can be grafted to solid supports to form oriented protein brushes that swell and collapse dramatically with changes in solution pH and ionic strength. We illustrate the value of sequence specificity by using proteases with mutually orthogonal recognition sites to modulate brush height in situ to predictable values. This study demonstrates that stimuli-responsive brushes can be fabricated from proteins and introduces them as a new class of smart biomaterial building blocks.

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Section: Discussionmentioning

confidence: 54%

Stimuli-sensitive intrinsically disordered protein brushes

et al. 2014

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“…Several techniques are based on this process. Surface-initiated polymerization (SIP) based on radical chemistry is commonly used [47,51,52]. "Grafting from" techniques yield higher grafted layer densities and overcome thickness limitations.…”

Section: Brush Structurementioning

confidence: 99%

“…The direct characterization of grafted polymer through the "grafting from" method is difficult. Generally, a sacrificial initiator is used, assuming that the polymer growth is similar on the surface and in the medium [51,52,58,59,[82][83][84][85]. Another method is to use reversible or breakable surface bonds in order to detach and study the grafted polymer [86].…”

Section: Grafting Techniques 321 Atom Transfer Radical Polymerizatmentioning

confidence: 99%

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

Conzatti

Cavalié

Combes

et al. 2017

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tBiomaterials surface design is critical for the control of materials and biological system interactions. Being regulated by a layer of molecular dimensions, bioadhesion could be effectively tailored by polymer surface grafting. Basically, this surface modification can be controlled by radical polymerization, which is a useful tool for this purpose. The aim of this review is to provide a comprehensive overview of the role of surface characteristics on bioadhesion properties. We place a particular focus on biomaterials functionalized with a brush surface, on presentation of grafting techniques for "grafting to" and "grafting from" strategies and on brush characterization methods. Since atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization are the most frequently used grafting techniques, their main characteristics will be explained. Through the example of poly(N-isopropylacrylamide) (PNIPAM) which is a widely used polymer allowing tuneable cell adhesion, smart surfaces involving PNIPAM will be presented with their main modern applications.

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“…These ionizable polymers can undergo drastic transformations in their structure, such as the formation of aggregates or swelling/deswelling of the polymers, upon pH variation [126]. Swelling/deswelling of polymers containing ionizable groups such as poly(methacrylic acid) (PMAA) [127,128] have been tethered to surfaces and used to control protein and cell adhesion. In a similar manner, surfaces based on poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) have been used to mediate protein adhesion, such as the net negatively charged bovine serum albumin (BSA) [129].…”

Section: Polymer Filmsmentioning

confidence: 99%

Stimuli‐Responsive Surfaces in Biomedical Applications

Pranzetti¹,

Preece²,

Mendes³

2013

Intelligent Stimuli‐Responsive Materials

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Nature provides mechanisms that are able to dynamically control specific and nonspecific interactions between cells and biological surfaces [1,2]. Scientists have long tried to reproduce these dynamic biological events and have recently made an important step in that direction by creating artificial stimuli-responsive surfaces [3][4][5][6][7]. These smart substrates present modulatory surface properties that are able to respond to external chemical/biochemical [8][9][10][11][12], thermal [13][14][15], electrical [16][17][18][19][20], and optical stimuli [21][22][23][24][25][26][27][28][29][30][31]. Due to their dynamic nature such substrates are very appealing for applications in the biomedical field [32]. Progress to date has led to control over biomolecule activity [33] and immobilization of a diverse array of proteins, including enzymes [34] and antibodies [35]. These prior achievements have encouraged researchers to take the challenge of using dynamic surfaces to modulate larger and more complex systems, such as bacteria [36] and mammalian cells [37].Achieving control over surface properties could provide new insights in the understanding of cell behavior and can offer distinct benefits with regard to the development of medical devices. For instance, the modulation of cell attachment and detachment could lead to the prevention of unwanted bacteria fouling on implants, reducing the risk of infections and rejection [38][39][40][41][42]. Furthermore, dynamic surfaces able to present on demand regulatory signals to a cell could provide unprecedented opportunities in studies of cell responses in real-time. Cells in tissues adhere to and interact with their extracellular environment via specialized cell-cell and cell-extracellular

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Stimuli-Responsive Surfaces Using Polyampholyte Polymer Brushes Prepared via Atom Transfer Radical Polymerization

Cited by 74 publications

References 45 publications

Stimuli-sensitive intrinsically disordered protein brushes

Stimuli-sensitive intrinsically disordered protein brushes

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

Stimuli‐Responsive Surfaces in Biomedical Applications

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