Stimuli-Responsive Injectable<i>In situ</i>-Forming Hydrogels for Regenerative Medicines

Kim, Da Yeon; Kwon, Doo Yeon; Kwon, Jin Seon; Kim, Jae Ho; Min, Byoung Hyun; Kim, Moon Suk

doi:10.1080/15583724.2014.983244

Cited by 70 publications

(30 citation statements)

References 313 publications

(247 reference statements)

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“…Photo‐responsive hydrogels can exhibit better performance, while large rigidity gradients (≥115 kPa/mm) ranging from ∼1 to 240 kPa can be produced by photo‐initiating polymerization of an acrylamide/bis‐acrylamide solution (Sunyer et al, ). Cell culture platforms which could recreate stiffness‐dependent microenvironments in vivo are rather attractive to engineers and researchers in the fields of tissue engineering (Sood et al, ), cell biology (Tam et al, ), regenerative medicine (Kim et al, ), and drug delivery (Naderi‐Meshkin et al, ).…”

Section: Introductionmentioning

confidence: 99%

Smart hydrogels with high tunability of stiffness as a biomimetic cell carrier

Zhao

Zhu

et al. 2019

Cell Biology International

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Human tissues are sophisticated ensembles of various distinct cell types encapsulated in the biomechanical cues of the extracellular matrix. It has been known matrix stiffness plays a pivot role in cellular events and tissue-scale biological processes. Thus, materials that can mimic mechanical environments of tissues in vitro and possess wide, physiologically relevant elasticity are highly desirable. Hydrogels provide a good cell platform to mimic native cellular environment. However, the limited stiffness tunability, and hinders the efforts to reproduce the biomechanical microenvironment of many in vivo progresses. These problems have been addressed by the recently emerged great quantity of exquisitely designed smart hydrogels. Smart hydrogels that respond sensitively to external stimuli are good choices due to the convenience in regulating their mechanical properties. In this review, we summarize the latest progress in the development of stimuli-responsive hydrogels as a cell carrier (platform for cell culture) which spans a wide range of stiffness. Different kinds of smart hydrogels corresponding to various stimuli, including pH, temperature, light, metal ions, and forces, are introduced and their stiffness modulation through physicochemical procedures are reported.

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Section: Introductionmentioning

confidence: 99%

Smart hydrogels with high tunability of stiffness as a biomimetic cell carrier

Zhao

Zhu

et al. 2019

Cell Biology International

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“…Among others, thermo-responsive polymer solutions are particularly attractive as specific injectable biomaterials when their spontaneous gelation occurs near physiological temperature of human body (thermo-gelation), without the requirement of any chemical treatment (Kim et al, 2014;Ruel-Gariépy and Leroux, 2004). Injectable gel-forming matrices do not require a surgical procedure for placement and bioactive molecules or cells can be incorporated simply by mixing before injection.…”

Section: Introductionmentioning

confidence: 99%

Rheological analysis of thermo-responsive alginate/PNIPAAm graft copolymers synthesized by gamma radiation

Lencina

Rizzo

Demitri

et al. 2019

Radiation Physics and Chemistry

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Focused on biomedical applications of thermo-responsive polymers, low-doses of gamma radiation from a 60 Co source were applied in a simple one-pot method to synthesize graft copolymers of alginate and poly(N-isopropylacrylamide) (PNIPAAm) with different compositions. The molar percentage of grafted NIPAAm (% molar NIPAAm) was determined by thermogravimetric analysis (TGA) and elemental analysis (EA), being the copolymer structure-property relationship studied in terms of thermo-associative and rheological behavior in aqueous solutions. The addition of more NIPAAm monomer in the initial mixture of reaction, as well as, increasing absorbed dose lead to a greater grafting. However, increasing radiation dose produces copolymers with diminished viscoelastic properties caused by the alginate backbone scission. From rheological curves, two transition temperatures, Ta and Tgel, were determined as a consequence of the thermo-responsiveness of PNIPAAm side chain. Storage (G') and loss (G'') modulus curves undergo a slope inversion at Ta temperature, where both moduli begin to increase caused by an associative behavior of PNIPAAm domains. While, Tgel temperature is related to the onset of the gelation process at the G'/G'' crossover. In order to design samples with liquid-gel transitions close to the human body, able to form gels in situ once inoculated, it was possible to tailor both transition temperatures selecting copolymers with an appropriate PNIPAAm content and optimizing the copolymer concentration in the aqueous solution. A good agreement between transition temperatures and viscoelastic properties was achieved for 5 wt% aqueous solutions of copolymers with low NIPAAm content synthesized at the lowest absorbed dose (0.5 kGy). Highlights  Low doses of γ radiation allow to synthesize alginate-g-PNIPAAm copolymers  Structure-properties relationship was well defined by rheology in aqueous solution  Copolymers sol-gel transitions could be tailored close to body temperature  We obtained injectable solutions to form gels in situ once inoculated

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“…MP diblock copolymers with PCL molecular weights (2410 g/mol) using MPEG (750 g/mol) were prepared via a previously reported block copolymerization method [8].…”

Section: Synthesis Of Mpeg-b-pcl Diblock Copolymer (Mp)mentioning

confidence: 99%

“…Among them, block copolymers consisting of (methoxy)polyethylene glycol (MPEG), poly(propylene oxide), and biodegradable polyesters, such as poly(L-lactic acid) (PLLA), poly(glycolic acid), their copolyesters, or poly(ε-caprolactone) (PCL), have been reported as potential thermogelling candidates [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Then, we examined the solution-to-hydrogel phase transitions of MP-Cl, MP-N 3 , and MP-NH 2 as a function of temperature to understand how the type and amount of pendant groups and the solubility and crystallinity of MP-Cl, MP-N 3 , and MP-NH 2 stabilize or destabilize aggregation. polyesters, such as poly(L-lactic acid) (PLLA), poly(glycolic acid), their copolyesters, or poly(ε-caprolactone) (PCL), have been reported as potential thermogelling candidates [8][9][10][11][12]. Previously, we developed MPEG-b-PCL (MP) and MPEG-b-polyester diblock copolymers, (MPEG-b-(PCL-ran-PLLA), MPEG-b-(PCL-ran-poly(trimethylene carbonate)), and MPEG-b-(PCLran-poly (1,4-dioxan-2-one)), as thermogelling materials [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Pendant Group-Functionalized Diblock Copolymers with Adjustable Thermogelling Behavior

Lee

Park

et al. 2017

Polymers

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Abstract:Recently, several thermogelling materials have been developed for biomedical applications. In this study, we prepared methoxy polyethylene glycol (MPEG)-b-(poly(ε-caprolactone)-ran-poly(2-chloride-ε-caprolactone) (PCL-ran-PfCL)) (MP-Cl) diblock copolymers at room temperature via the ring-opening polymerization of caprolactone (CL) and 2-chloride-ε-caprolactone (fCL) monomers, using the terminal alcohol of MPEG as the initiator in the presence of HCl. MPEG-b-(poly(ε-caprolactone)-ran-poly(2-azide-ε-caprolactone) (PCL-ran-PCL-N 3 )) (MP-N 3 ) was prepared by the reaction of MP-Cl with sodium azide. MPEG-b-(poly(ε-caprolactone)-ran-poly(2-amine-ε-caprolactone) (PCL-ran-PCL-NH 2 )) (MP-NH 2 ) was subsequently prepared by Staudinger reaction. MP-Cl and MP-N 3 showed negative zeta potentials, but MP-NH 2 had a positive zeta potential. MP-Cl, MP-N 3 , and MP-NH 2 solutions formed opaque emulsions at room temperature. The solutions exhibited a solution-to-hydrogel phase transition as a function of the temperature and were affected by variation of the chloride, azide, and the amine pendant group, as well as the amount of pendant groups present in their structure. Additionally, the phase transition of MP-Cl, MP-N 3 , and MP-NH 2 copolymers was altered by pendant groups. The solution-to-hydrogel phase transition was adjusted by tailoring the crystallinity and hydrophobicity of the copolymers in aqueous solutions. Collectively, MP-Cl, MP-N 3 , and MP-NH 2 with various pendant-group contents in the PCL segment showed a solution-to-hydrogel phase transition that depended on both the type of pendant groups and their content.

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Stimuli-Responsive InjectableIn situ-Forming Hydrogels for Regenerative Medicines

Cited by 70 publications

References 313 publications

Smart hydrogels with high tunability of stiffness as a biomimetic cell carrier

Smart hydrogels with high tunability of stiffness as a biomimetic cell carrier

Rheological analysis of thermo-responsive alginate/PNIPAAm graft copolymers synthesized by gamma radiation

Preparation of Pendant Group-Functionalized Diblock Copolymers with Adjustable Thermogelling Behavior

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