Stimulatory Effect of the Lysosomal Stabilizer, Chloroquine, on the Respiration and Motility of Fresh and Aged Bovine Spermatozoa

Norman, C.; Gombe, S.

doi:10.1530/jrf.0.0440481

Cited by 16 publications

(16 citation statements)

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“…This effect has also been shown to be enhanced in resistant Plasmodium strains [23]. It is interesting to note that chloroquine also influences mitochondrial activity by stimulating respiration in bovine spermatozoa [24]. …”

Section: Resultsmentioning

confidence: 99%

Yeast Sensors for Novel Drugs: Chloroquine and Others Revealed

Swart

Olivier

Dithebe

et al. 2012

Sensors

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In this study the mitochondrion is regarded as a target to reveal compounds that may be used to combat various diseases. Consequently, the sexual structures of yeasts (with high mitochondrial activity) were identified as sensors to screen for various anti-mitochondrial drugs that may be toxic to humans and that are directed, amongst others, against fungal diseases and cancer. Strikingly, these sensors indicated that chloroquine is a potent pro-mitochondrial drug which stimulated yeast sexual reproduction. In addition, these sensors also showed that some Non-Steroidal Anti-Inflammatory drugs (NSAIDs), anti-malarial drugs, antifungal and anticancer drugs are anti-mitochondrial. These yeast sensor bio-assays may fast track studies aimed at discovering new drugs as well as their mechanisms and should now be further evaluated for selectivity towards anti-/ pro-mitochondrials, fertility drugs and contraceptives, using in vitro, in vivo, in silico and omics research.

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Section: Resultsmentioning

confidence: 99%

Yeast Sensors for Novel Drugs: Chloroquine and Others Revealed

Swart

Olivier

Dithebe

et al. 2012

Sensors

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“…In livestock animals such as porcine and bovine, short- term or low-dose treatment resulted in an increase in sperm motility and fertility (Norman and Gombe, 1975;Egbunike, 1982). Moreover, chloroquine can be used in an artificial insemination programme to enhance conception of stored porcine sperm (Egbunike, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…The effects of chloroquine on sperm are time and dose-dependent, as short-term or low-dose treatment resulted in an increase in sperm motility and fertility in mammalians (Norman and Gombe, 1975;Egbunike, 1982), whereas long-term or high-dose treatment resulted in a dose dependent decrease in sperm motility and fertility (Adeeko and Dada, 1998;Okanlawon et al, 1993). However, molecular mechanisms of chloroquine for increasing sperm motility and successful fertilization remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish

Jin

Xie

et al. 2017

Aquaculture

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“…Caffeine, a metabolic stimulant enhances motility, respiration and fructolysis of epididymal and ejaculated spermatozoa of cattle and buffalo bulls [11]- [13]. It has also been reported to improve progressive motility of both fresh and preserved sperms of various species [14] [15]. Chloroquine diphosphate, a membrane stabilizer is known to stimulate the respiration and motility of fresh and aged spermatozoa in vitro [16] and to improve the post thaw quality of frozen semen [17].…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Ascorbic Acid, Caffeine and Chloroquine Diphosphate in Dilutor Improves Structural and Functional Status of Frozen Semen

Srivastava¹,

Kumar²

2014

OALib

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Effect of supplementation of ascorbic acid, caffeine and chloroquine diphosphate in the dilutor on post-thaw quality of spermatozoa was studied. Thirty-two semen ejaculates (8 each from 2 Holstein Friesian and 2 crossbred; 50% Friesian x 50% Haryana) were studied. Ascorbic acid, caffeine and chloroquine diphosphate were added in Tris-egg yolk-glycerol (control) extender at the concentration of 10 mM, 0.54 mM and 7 mM, respectively. The post-thaw semen parameters studied were structural (acrosomal integrity and livability), functional [motility, per cent sperm responding to hypo-osmotic swelling test, i.e. HOS positive sperm and penetrability of sperm in bovine cervical mucus, i.e. sperm penetration distance-SPD] and measurement of activities of Glutamic oxaloacetic transaminase (GOT), Glutamic pyruvic transaminase (GPT), Alkaline transaminase (AKP) and Acid transaminase (ACP). Certain enzymes in the seminal plasma. The semen from crossbred bull had significantly lower mass activity (P ≤ 0.01), initial progressive motility (P ≤ 0.01), sperm penetration distance (SPD value) (P ≤ 0.01), HOS positive sperm and seminal plasma GOT activity, but higher abnormal sperm. Incorporation of additives in the dilutor significantly improved the post-thaw semen quality, especially the post-thaw motility, post-thaw per cent live sperm, sperm penetration distance and sperm responding to hypo-osmotic solution. The leakage of all the 4 enzymes (GOT, GPT, ACP and AKP) was significantly less in the samples frozen in presence of ascorbic acid and chloroquine as compared to control. Ascorbic acid resulted in maximum improvement to the quality of frozen-thawed semen followed by chloroquine and caffeine. The study concludes that post-thaw quality of frozen semen of HF and crossbred bulls significantly improves if the dilutor is supplemented with 10 mM concentration of ascorbic acid.

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Stimulatory Effect of the Lysosomal Stabilizer, Chloroquine, on the Respiration and Motility of Fresh and Aged Bovine Spermatozoa

Cited by 16 publications

References 1 publication

Yeast Sensors for Novel Drugs: Chloroquine and Others Revealed

Yeast Sensors for Novel Drugs: Chloroquine and Others Revealed

Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish

Incorporation of Ascorbic Acid, Caffeine and Chloroquine Diphosphate in Dilutor Improves Structural and Functional Status of Frozen Semen

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